The Effect of Titanium on Oleic Acid Transport in Rat EGS

There are reports indicating that fatty acid transport is affected by many biochemical parameters including trace metals. In this study the effect of Ti on oleic acid transport is investigated Male rats (200-250gr) were used for the experiments. Rats were killed, their intestines were removed and the jejunum parts were dissected. Everted Gut Sacs (EGS) were prepared from this part. Sacs were filled with buffer and incubated in a medium containing oleic acid and TiCl3. The transported oleic acid inside the EGS was measured spectrophotometrically under different condition of PH, temperature and TiCl3 concentrations. Titanium decreased fatty acid uptake by entrocytes in a dose dependent manner. In a medium contained 0.5, 1, 1.5, 10 mmol of TiCl3, uptake of oleic acid was inhibited by 14.6%, 31.6%, 38.6% and 54.5% respectively. This inhibition was achieved when sodium ion (Na) was present. In the absence of sodium, uptake of oleic acid was inhibited by 3.5%, 28%, 29%, and 39% respectively which was not very significant. Oleic acid transport appeared to be an Na dependent process and Ti may exert its inhibitory effect by interfering with this system.