Effect of adenosine At receptors activity of nucleus accumbens on amygdala kindled seizures in rats

The role of adenosine AI receptors of the nucleus accumbens on amygdala kindled seizures was investigated. All animals were implanted with a triplolar (stimulating and recording) electrode in the amygdala and tow guide cannula in the nucleus accumbens. Animals were kindled by daily electrical stimulating of the amygdala. In the full kindled animals, N6 - cyclohexyladenosine (CHA; 1,5, 10 M), an adenosine AI receptor agonist, was injected bilaterally into the nucleus accumbens. Obtained results showed that CHA at the concentration of 10M reduces the amygdale afterdischarge duration (A-ADD), at 5, 15, 60 and 120 min post drug injection. It also decreased the stage 5 duration (S5D), but reduced the accumbens afterdischarge duration (Acc-ADD), at 15 min post drug injection, and increased the latency of the stage 4 seizure (S4L) at 5 and 15min post drug infusion. In addition, at the concentration of 5 M, the S4L was increased and the SsD was decreased at 5 and 15 min post drug injection. The bilateral injection of 8- cyclopenthyl-l, 3- dimethylxanthine (CPT), an adenosine AI receptor antagonist, at the concentration of 5 M into the nucleus accumbens did not alter the seizure parameters. The intra- nucleus aecubens infusion of CPT (5 M), 5 min before CHA (10 M) blocked the anticonvulsant effects of CHA. These results suggest that the nucleus accumbens has a role in the seizure propagation from the amygdala and it's adenosine AI receptors activity has anticonvulsant effects in amygdala kindled rats.