Cytotoxicity evaluation of ProRoot MTA, Root MTA and Portland cement on human gingival fibroblasts

The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA)، Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs).

The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well plates. Cellular viability was assessed using MTT assay in different intervals (freshly mixed، 4، 24، and 168 hours after mixing). The data were analyzed using the One-way ANOVA and Tukey’s test at 95% significance level.

It was indicated that there was not a significant difference in cytotoxicity of test materials (p>0. 05). In addition، there was not a statistically significant difference between different time intervals within each group (p>0. 05).

PMTA، RMTA and PC showed comparative biocompatibility while evaluated in-vitro