Determination of Relative Population of Bifidobacterium spp. in Broiler Gut Using Densitometry Technique Based Molecular Genetics

Today, it is used modern molecular techniques for investigation on gastrointestinal bacteria. Bifidobacterium spp. have critical role in prevention of some pathogens colonization in gastrointestinal tract's poultry. These bacteria increase poultry resistance against diseases and improve their immune system and performance. This experiment was conducted in order to determining of relative population of Bifidobacterium spp. in duodenum, jejunum, ileum and cecum of broiler chickens at 2007 in Islamic Azad University, Rasht Branch. It was removed gut contents and extracted their DNA. It was obtained a specific band for detecting of mentioned bacterium using a set specific primer and also a specific band for detecting of all bacteria. It was determined relative population of Bifidobacterium spp. relative to total gut bacteria. Result analysis was showed Bifidobacterium spp. consists 4.50% of total duodenum bacteria. Also it was showed Bifidobacterium spp. consists 4.95% of total jejunum bacteria. Meanwhile Bifidobacterium spp. consists 5.17% of total ileum bacteria. Furthermore it was showed Bifidobacterium spp. consists 9.36% of total cecum bacteria. Relative population of Bifidobacterium spp. in lower segments i.e. ileum and cecum were higher than upper segments i.e. duodenum and jejunum. Meanwhile, relative population of Bifidobacterium spp. in 4, 14 and 30d of ages in cecum were higher than other segments i.e. duodenum, jejunum and ileum. Obtained results showed Bifidobacterium spp. populations were variable in various intestine segments and correlated with their functions and physicochemical environment.