Detection of bacteria responsible for gallbladder inflammation and gallstones

The most prevalent complication of gallstone is chronic cholecystitis. The aim of the present study was to determine the responsible microorganisms in patients undertook cholecystectomy and determine their antibiotic resistance pattern. Cholecystectomy was achieved in 100 patients. Collected samples transferred to laboratory in appropriate media, then cultured on selective media to isolate the possible causative bacteria. Antibiotic susceptibility tests were also performed by Kirby-Baur method with a colony in Muller-Hinton agar medium. Totally, 63 samples showed bacterial growth, of which 11 revealed two species of bacteria. A total of 10 gram positive (enterococci and staphylococci) and 63 gram negative (E.coli, klebsiella, aerobacter, pseudomonas, proteus, citrobacter, providencia and acinetobacter) bacteria were isolated. Isolating bacteria and determining their susceptibility to different antibiotics may help physicians to manage cholecystitis and its associated complications.