Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation
Author(s):
Abstract:
Objective
The aim of the present study was to investigate the effect of protein and DNA components of Toxoplasma gondii on maturation of dendritic cells and their efficiency in IL-12 production and proliferation of T cells. Materials And Methods
for DC generation, Bone marrow cells were cultured in the presence of GM-CSF and IL-4 for 5 days. Tumor lysate and protein or DNA components of Toxoplasma gondii were added to the culture media and incubated for another 2 days. LPS was added as control for DC maturation. Proliferation of T cells were determined by MLR and IL-12 production was measured by ELISA kit. Maturation of dendritic cell were determined by flowcytometry. Results
DCs treatment with protein components of Toxoplasma gondii caused a significant increase in IL-12 production and proliferation of T cells (P<0.001). Conclusions
Different compositions of microbial body like protein and DNA components of Toxoplasma gondii can cause augmentation of antigen presentation capacity of DC and their IL-12 production capability. Among these components the protein was more effective as compared to DNA.Language:
Persian
Published:
Journal of Pathobiology Reaearch, Volume:12 Issue: 3, 2009
Pages:
1 to 8
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