The roll of bacterial ACC deaminase enzyme on the alleviation of negative effects of salinity on canola growth

In this study a greenhouse experiment was conducted to evaluate the role of ACC deaminase enzyme on alleviation of salinity stress of canola plant. Canola plants were exposed to Pseudomonas fluorescens strain P12 which contains a rhizobacter capable of producing Pseudomonas fluorescens in comparison with plants exposed to mutant Pm12 unable to produce the required enzyme. The mutant was chemically produced using Ethyl Methane Sulfonate (EMS). Comparison of wild strain P12 with its mutant Pm12 indicated that under both saline and non-saline conditions, strain P12 significantly enhanced canola growth parameters including shoot fresh and dry weight, root dry weight, shoot length and green area index. In addition, canola plants inoculated with strain P12 absorbed higher rates of sodium, potassium, calcium, and magnesium, which can be attributed to enhanced roots growth. However, comparing strains P12 with Pm12 indicated that the production of ACC deaminase enzyme by P12 enhanced canola salinity tolerance and consequently, its growth.