Isolation and Identification of the Different Species of Flavobacterium from the Rhizosphere of Wheat Cultivated in the Different Regions of Iran

AbstractAmong rhizospheric bacteria, great attention has been paid to the group of plant growth promoting rhizobacteria (PGPR), and their role in increasing the growth and health of plants. Therefore, it is used largely as inoculum all over the world. The rhizospheric bacterium, which has the genus of Flavobacterium, and promotes plants growth, has been studied in recent years. In the present research, 65 samples of rhizospheric soil were taken to isolate Flavobacterium from the rhizosphere of wheat cultivated in different regions of Iran. To isolate, cultivate, and preserve Flavobacterium, different and diverse formulations such as ATCC 647, 65 ATTC M1, and M1 Medium, which were recommended for this bacterium, were used for the development of a new specified culture media (FIM). Sixty-one isolates attributed to Flavobacterium were isolated and purified using specified culture medium (FIM). The genus and species were identified through microscopic, physiological and biochemical tests. The results obtained from our tests indicated that there are 5 species F. multivorum, F.odoratum, F. thalpophilum, F.balastinum and F. indoltheticum in the rhizosphere of wheat. Among the isolated species, F.odoratum showed the most frequency (72 percent), and the F. thalpophilum and F. balastinum and F. indoltheticum possessed the least frequency (1.6 percent).