Effects of watery and alcoholic extract of green tea on the process of open skin wounds healing in male rat (NMRI)

According to several documented usefulness of green tea and importance of healing of open skin wound, this study designed to evaluate effects of watery and alcoholic extracts of green tea on the healing of open skin wounds of NMRI mice. In this experimental study, 56 male NMRIs divided equally in 7 different groups, were studied. Different doses of watery and alcoholic extracts, including 50, 150 and 300 μL, were injected to 6 experimental groups. After anaesthetizing the NMRIs, skin wound was created on the back of the rats by a 6mm punch. Control group was treated by physiology serum around the wound of the back of each NMRIs. Dimension and also the recovery percent of the wound in the 1st, 3rd, 5th, 7th, 10th, 13th and 15th days of study, were measured. Furthermore, the needful time for recovery was evaluated. Two Specimens of wound skin were supplied in 4th, 7th and 15th days of study for microscopic studies. Fibroblasts, inflammation, epitheleum and endothelial cell of blood vessels from the wounds were studied. Data were analyzed using analysis of variance (ANOVA) method. P<0.05 was considered as significant level. There were no significant differences between three groups of control, watery extract and alcoholic extract groups in recovery processes (NS). But, mean diameter of wound within 1, 3, 5, 7 and 15 days of study was significantly lower in experimental groups compared with control group (p<0.001). Degree of fibroblasts, inflammation and epithelium in different days in 6 groups of watery and alcoholic groups was significantly lower than control group (p<0.001). This study showed that watery and alcoholic extracts of green tea speed up the wound healing in NMRIs and there isnt any difference between the use of watery and alcoholic extracts. But higher dose of injection (300 μL) is valuable in forming of wound.