Variations of protein banding patterns of HMW glutenins using chromosomal substitution lines of Triticum aestivum

heat seed contains gliadines and glutenins. Glutenins is consisted of high molecular weight and low molecular weight. Total protein was extracted from 27 substitutions Lines related to kapla – shayen substitutions lines and Chinese Spring as control samples using laemmli protocol. After protein extraction, the samples were electrophoresed using SDS-PAGE. The gels were stained using commassie blue R250 and silver nitrate methods; then similarity matrix was calculated using Jaccard coefficient. Payne scoring system was calculated for each line. The range of coefficients of similarity was 0.55 -1. The coefficients of similarity were 1 in the most of lines. The coefficients of similarity of substitution lines of kapla and parent were 1 but it was 0.736 for kapla 3A. The coefficient of similarity of kapla and other lines was low. These lines and controls were clustered to 3 groups that kapla 3A and shayen 4b were in 2 separately and single groups. Based on silver staining, the range of coefficients of similarity varied from 0.7-1. The coefficients of similarity of almost all lines were 1. Kapla 3A, kapla 4B and shayen 7B had lowest similarity. According to cluster analysis, these lines and controls were clustered to 2 groups that kapla 3A was in the separately group. Based on Payne scoring system, only kapla 3A had 2* and 10 bands that can be main reason of difference to other lines and control. According to these scoring the bands of all lines (except kapla 3A) were similar to parent kapla and Chinese Spring; but some of lines of shayen and control were different and these lines were clustered to 3 groups that shayen 7A, shayen 4B and kapla 3A were located in separately group. Totally, in the kapla, 3A chromosomes and in shayen, 7A and 4B chromosomes had high variations in the band patterns.