Evaluation of Genetic Diversity By Using of Link Maker For Amylase Content of Some Iranian Local Rice Cultivars

Molecular markers are the best method for investigating the genetic diversity. In thisexperiment, 72 cultivars including Indica and Japonica were investigated in RiceResearch Centre of Iran. In order to evaluate the genetic diversity of locus waxy linkedto the trait controlling the amylose content, PCR was performed using twooligonucleotides (484 and 485) and scored. The important Iranian cultivars of rice werescreened using waxy microsatellite marker and classified into seven groups based on(CT)n repeats ranging from n=7 to 20. The amplified PCR products ranged from 102 to128 bps in length and represented the (CT)n repeats of (CT)7, (CT)8, (CT)14, (CT)17,(CT)18, (CT)19 and (CT)20, that were according to amylose content of cultivars in Iraniangermplasm classified in seven groups for that locus and explained 70%, 72%, 78.95%,80% and 70% of each group variations, respectively.