Lymphocyte Cytotoxicity of oxLDL in Patients with Atherosclerosis
Message:
Abstract:
Background
Atherosclerosis, a chronic inflammatory disease of the vessel wall ischaracterized by local and systemic immune responses to a variety of antigens. Oxidized lowdensity lipoprotein (oxLDL) is considered as an important determining factor in thepathogenesis of atherosclerosis.
Objective
The purpose of this study was to investigate the degree of peripheral blood mononuclear cells (PBMC) vulnerability to in vitro oxLDL-inducedcytotoxicity from atherosclerotic patients in comparison to healthy individuals.
Methods
Thirty patients with atherosclerotic lesions, confirmed by angiography, and 30 matched healthy individuals were investigated. PBMC was prepared from individual's blood samples which were further stimulated with low dose (1 μg/mL) and high dose (50 μg/mL) of extensively oxidized LDL. MTT assay was utilized to measure cell viability and proliferation. Stimulation index (SI) was calculated as mean ratio of optical density (OD) of the stimulated cells divided by OD of untreated cells.
Results
Low dose oxLDL treatment caused no significant proliferative or cytotoxic effect in the control group; however, similar treatment caused significant cytotoxic effect in the patient group compared to the controls (p=0.026). High doseoxLDL treatment induced more significant cytotoxicity in the patient compared to the control group (p=0.006). Comparison of the SI between the two groups of patients and controls showed significantly lower index by either the low (p=0.03) or the high dose (p<0.001) oxLDL in the patients compared to the controls.
Conclusions
PBMC from patients with atherosclerosis showed increased susceptibility to oxLDL-induced cytotoxicity. Our results imply that prolonged exposure to elevated levels of circulating oxLDL could weaken the cellular defense mechanisms by progressive depletion of the pool of antiapoptotic proteins, rendering the cellsmore vulnerable to oxLDL-induced cell death.
Language:
English
Published:
Iranian journal of immunology, Volume:8 Issue: 1, Winter 2011
Pages:
27 to 33
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