Evaluation validity of Easygluco glucometer to determine Glucose in Equine practice

Serum glucose level considered as a prognostic indicator in a variety of equine diseases such as colic, hypoglycemic conditions, septicemia, diarrhea, diabetes mellitus. Considering the emergency nature of disorders mentioned above, fast and accurate measurement of this parameter is necessary. Self-monitoring of blood glucose (SMBG) allows diabetic patients to achieve and maintain specific glycaemic goals. There are several glucometer devices in the market, all of human kind and there's high competition between companies. ,several studies has performed to evaluate efficacy of this device in medicine. Unfortunately limited studies has been done in veterinary medicine. The aim of this study was to evaluate validity of validation EasyGluco glucometer to measure blood glucose levels and it's application in equine clinical practice. Fourthy five horses warm blooded healthy and sick horses (horses in the hospital) with different ages were selected. 3 ml of blood were collected in the tube containing sodium fluoride anticoagulant. Glucose concentration of capillary and venous blood samples were determined Immediately with glucometer Plasma immediately separated and transferred, on ice to the laboratory & kept at - 20 ° C until laboratory examination. The level of plasma glucose were determined by photometric method using Pars Azmoon kit. To evaluate repeatability, accuracy and Within run imprecision of Easygluco glucometer, one sample were analyzed for 15 consecutive time at intervals of 15 seconds and CV were determine & were compared with control samples. CV in blood samples taken, low and high controls were 4.1, 3.3 and 4.4 percent respectively in which all were acceptable with NCCLS standards. In order to Evaluate the diversion of glucometer results with standard methods, Bland - Altman Plot was performed. Bias levels in venous and capillary glucometer methods with standard methods, respectively, were -9.4 and -34.8. To determine the correlation between the results of the glucometer and laboratory method, correlation coefficient determined. In this study, the correlation coefficient (r) between photometer method and venous blood glucometer device was 0.823. The results of this study revealed lower correlation compare to human studies. It Seems that it might be due high level of hematocrite in the horse & human calibrated device. In order to overcome this problem, Correction Factor(CF) should be calculate. CF obtained in this study In this study WAS 1.1. Th results of this study indicates that correction factor should be applied, then after glucometer results can be trusted