Quantitative expression analysis of some Fusarium head blight resistance candidate genes in response to Deoxynivalenol (DON)

This study was carried out to determine quantitative differences and expression pattern of three candidate gene for resistance to Fusarium Head Blight in response to Deoxynevalenol (DON), the main mycotoxin product fo F. graminearum the causal agent of FHB. The results showed that 10 mg/l DON has not significant effect on germination of resistant and susceptible varieties of wheat. But 5-15 mg/l concentration of DON caused significant reduction in fresh and dry weight of resistant, semi resistante and susceptible plantlets. The effect of DON on quantitative expression of three resistance candidate genes including PDR5- NPR1- Xsts3B-138_1 was studied with Real time PCR. The increase in expression of PDR5 was significant after DON treatment in Falat (susceptible) and Frontana (semiresistant). NPR1 expression was reduced significantly after DON treatment in all varieties. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 was reduced in Falat and Frontana after DON treatment but not in Wangshuibai