Evaluation of quantitative changes in transcript level of two genes encoding transcription factors involved in expression of salinity tolerance genes in wheat seedling

Message:
Abstract:
alinity is one of the most important abiotic stresses which deduced crop yields annually around the world, especially Iran. Most of the crop plants are susceptible to salinity and can not survive under very acute salinity, if survive they have very low products. Based on different studies, several genes with different function are induced in response to salinity and several transcription factors involved in regulation the expression of these genes. In this study, the expression pattern of two genes which their product is essential transcription factors for expression of salinity tolerance genes, were investigated using QRT-PCR. Sampling was done from 8-days seedling of tolerant and susceptible wheat varieties which were grown in -0.25, -0.5, -0.75, -1, -1.25 and -1.5 MPa of salinity treatment induced by NaCl as well as distilled water treatment as control and the expression pattern of TaDBP and TaDREB2 genes were studied. These genes belong to AP2/EREBPs transcription factor family. Results showed that, TaDBP was up-regulated in tolerant variety (Kavir) which means the product of this gene can act as a transcription factor to induce the promoter and transcription of salinity tolerance genes. In contrast, the expression pattern of TaDREB2 gene showed that this gene can be as a repressor transcription factor in expression of genes involved in salinity tolerance in studied variety.
Language:
Persian
Published:
Journal of Genetics, Volume:5 Issue: 4, 2011
Page:
89
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