Callus induction, Somatic embryogenesis and plant regeneration in fenugreek (Trigonella foenum-graecum L.)

In order to study callus induction, somatic embryogenesis and plant regeneration in fenugreek (Trigonella foenum-graecum L.) an experiment was conducted in Sari Agricultural Sciences and Natural Resources University during 2008. In the current experiment, different explants including stem segments, embryos, hypocotyls, etc. were cultured on two basal culture media viz. B5 and MS. Moreover, 2, 4-D, Kin, NAA, BAP and IBA were used as plant growth regulators. Result showed that the medium containing 2mgl-1 2, 4-D in combination with 0.5mgl-1Kinetin was the best treatment for callus induction in both MS and B5 media. Combination of 0.5mgl-1 NAA and 2.5mgl-1 BAP was the best treatment for somatic embryogenesis in both basal media. Also, combination of 1.5mgl-1 BAP and 0.5mgl-1 NAA was the best hormonal treatment to shoot regeneration in both basal media. According to the results, the treatment containing 1 mgl-1 IBA was optimum for root induction from regenerated shoots on MS medium.