Association analysis of agronomic traits with microsatellite markers in Iranian barley landraces barley

In this study, association of agronomic traits and molecular markers in barely was studied using 115 landraces and 70 SSR markers. These markers were produced using 10 primer pairs, where PIC values for primers ranged from 0.31 to 0.85. AF220725A, Bmag0013 and HVM40 primers with the maximum PIC values showed higher polymorphic index, indicating that these markers display the genetic distance of varieties better than other markers. By multiple regression method (stepwise) relationship of each 10 agronomy traits and the 70 polymorphic markers were studied. HVM20, Gms003, Bmac0306 and HvHVA1 primers, explained more variation of the traits compared to other primers. Moreover, our results showed that some of the markers were related to more than one trait, implying close linkage and/or peliotropy of these traits on chromosomes. However, for better understanding of these relationships, preparation of segregating population and linkage mapping is necessary