Study of Genetic Variation within Red Jabal Barez Goat Population Using Microsatellite Markers

Genetic diversity of Red Jabal Barez red goat population was studied based on eight microsatellite markers consist BM1312, MAF64, ILSTS034, ILSTS059, OraFCB20, IL2RA, LSCV24 and LSCV11. Blood sample extracted of 100 Red Jabal Barez goats were randomly collected from 10 farms and DNA was isolated using the Diatom DNA PREP kit. The PCR reactions were successfully done and all loci showed suitable polymorphism with 7.62 alleles, on average. Five loci of LSCV11, ILSTS034, ILSTS059, OraFCB20, IL2RA were at Hardy-Weinberg disequilibrium (P<0.05). In this population, hetrozygosity varied from 0.7616 at IL2RA to 0.8743 at OraFCB20 locus and the same loci had the lowest and the highest diversity based on Shannon index and polymorphic information content (PIC), respectively. Number of observed and effective alleles and expected hetrozygosity were also calculated for each locus. Average hetrozygosity was estimated as 0.81 for this population. It can be concluded that Red Jabalbarez goat benefits from approximately high genetic variation. Therefore we can well use this potential in conservative and breeding programs for this population.