Study of Micropropagation of Anthurium Andreanum Using Tissue Culture

Aim and Background. Ant hurium andreanum is a perennial herbaceous plant, and an economically important genus of Araceae. Tissue culture of Anthurium andreanum offers an alternative tool for rapid multiplication in a short period. Materials and Methods. Leaf plate explants of pink cultivar were used. The leaves were cut into 1×1 cm sections. Segments of leaf were sterilized with 1% NaClO for 20 minutes. Explants were cultured on MS medium fortified with IAA (0.1- 0.4 mg/l) and BA (0.25 -1.5 mg/l), 30 g/l sucrose and 8 g/l Agar for callus induction in dark conditions. The experiment was conducted as a completely randomized design (CRD) with factorial arrangement and 8 replications. Callus was cultured on MS and ½ MS without hormones base media for shoot induction in light condition. The shoots transferred to rooting media after regeneration.Results. Best result for callus induction was obtained from MS medium containing supplement of 0.2 mg/l IAA and 1 mg/l BA. Also the ½ MS media showed the best result for a number of shoots. The Best result for rooting in vitro was in ½ MS medium with 1g/l activated charcoal without hormones.Conclusion. All shoots formed roots after 30 days and Plantlets were transferred into pot with perlite bed and grown in the greenhouse.