Application of Single Wall Carbon Nanotube for Synthesis of Glucose Biosensor

In this paper, the carbon nanotube was applied to prepare an appropriate bed for immobilization of Glucose Oxidase enzyme. The more operational advantages of glucose oxidize enzyme matrix include: efficiency and more accurate control over enzyme reaction, protection against destruction and waste of enzymes, application of enzyme matrix with high efficiency and control of enzymic reaction. For immobilization and stabilization of enzyme on the bed, the ready matrix was washed by sulfuric acid, nitric acid and enough ionized water. The matrix surface, for restrain distort enzyme, was covered by Sistamine. The glucose oxidize enzyme was connected on the matrix by intermediate material; one side of intermediate material is amid group and another side is pyren group. For elimination of impurity, the ready matrix was put into Dimethylformamide (DMF) for several hours. The activity parameter of enzyme was measured by Spectrophotometer in the 460 nm wave length; the immobilized enzyme was could stable for long time. This matrix can use as electrode in sensors structure.