Effects of different levels of multi-Enzyme on Liver enzyme changes and performance parameters in the grower's rainbow trout (Oncorhynchus mykiss)

Enzymes are organic catalysts, that can Trigger or accelerate chemical reactions andcovert One or more organic compounds to organic production. This study was designed toinvestigate the influence of supplementing multi-enzyme on liver biochemical parameters(LDH, ALP, AST, and ALT) and performance parameters of rainbow trout growers (150± 5 g). The multi-enzyme were incorporated into a commercial pellet ration to constitutethree experimental diets containing 500, 1000, 1500 gram multi-enzyme per one ton feedintake, while a fourth diet without the multi-enzyme supplement served as the control diet.Fish were sampled at 10, 20, 30 and 40 days after commencement of the feeding trial todetermine feed conversion ratio (FCR), weight and length rate and survival rate. At theend of the raising period 50 fish from each and finally 200 fish were selected randomlyand blood sampling performed and then liver biochemical parameters were measured.Results of our study indicated that effect of different supplementing multi-enzyme levelson feed conversion ratio (FCR), weight and length rate and survival rate, there was notany meaning relation in dose rates (p>0.05). In this study, 1500 g/ton group displayedsignificantly reduction (P<0.05) AST levels compared to 500 and 1000 g/ton groups. Theresult showed that 500g/ton group displayed significantly reduction (P<0.05) ALT levelscompared to control group. 500, 1000 and 1500g/ton groups displayed significantlyreduction (P<0.05) LDH levels compared to control group. Also, There was nomeaningful relationship between multi-enzyme level in ration ALP levels (P>0.05).According to this study, the use of multi-enzymes, can reduced amounts of ALT, AST andLDH levels in rainbow trout growers. Therefore, taking these supplements can berecommended, in considered values for use in aquaculture.