Effect of temperature on the pattern of antibiotic resistance in Escherichia Coli Isolates from poultry

Escherichia coli are highly pathogenic bacteria in human being and animals. Extraordinary Antibiotic consumption in animals and poultries for controlling of E-coli can cause antibiotic-resistance in these microorganisms. One of these resistance methods is plasmid-based resistance in these bacteria. Some of the plasmids are heat-sensitive and destroy in inadequate overheating. The main aim of this study was evaluation of antibiotic-resistance ability by using the heat in E-coli isolated from poultry. Among this study 25 E-coli (probable) samples isolated from infected broilers. Then samples transferred to bacteriology laboratory and cultured in Mc-Kanky agar specific medium. In next stage these samples were transferred to differential Enterobacteriaceae family media for identifying the bacteria species. Then isolates transferred in two different BHI media and each one simultaneously incubated in 37°c and 43.5 °c. In last stage the sensitivity to proper antibiotics were measured by using disk-diffusion (Kirby Bauer) method based on CLSI criteria. The results were evaluated in both incubated (37°c and 43.5 °c) groups in three different categories: sensitive, intermediate and resistant. The highly antibioticsensitivity in this survey was seen in incubated E-coli isolations with Chloramphenicol discs (52% & 56%) in both temperatures and greet antibiotic-resistance was observed in groups with Erythromycin 92% discs but no significant different was seen in reduction of bacterial antibiotic-resistance in two different temperatures incubated E-coli isolations.