Gene trapping techniques and their progress

Gene trapping technique is a powerful method for mutational engineering works onspecific genes in cell, which is defined as a kind of in vitro site directed mutagenesis.This method is used for gene knock out or analysis of gene function. Interestingly thisapproach can be used as therapeutic device for correction of mutated genes. Gene trapping has been staged in three steps: gene cloning, the study of the pattern of gene expression and the analysis of respective mutated phenotype. This method is based on homologous recombination. For detection of homologous recombination, several approaches like RT-PCR; RACE-PCR would be useful. Thus, designing a set of specific primers pairs, for marker gene and insetred gene is essential for such PCR methods