Evaluation of soybean mutant lines with high N2 fixation capability trough RAPD molecular marker

In this research genetic diversity of 33 M7 soybean mutant lines with high N2 fixation characteristicwhich evolved from L17 cultivar irradiated by γ ray (co-60) doses 150, 200 and 250 Gray (absorbeddose), L17 cultivar and two commercial cultivar; Clark (CL) and Williams (WI) were studied usingrandom amplified polymorphic DNA (RAPD) markers. RAPDs established 104 major amplified productsusing 10 polymorphic primers. Out of 104 markers, 34 were monomorph and the remaining (70) werepolymorph. To estimate the similarities amongst mutant lines, parent cultivar and commercial cultivars,the Jacard coefficient provided similarity values ranging from 0. 17 to 0.79. Clustering of studiedgenotypes with cluster analysis method and by CL (completely linkage) algorithm resulted in 7 majorgroups. Regarding to the similarity matrix, the mutant lines number 32 (M32) and number 9 (M9) werecloser and entirely different from parent cultivar respectively. According to the high genetic variability of these mutant lines, they can use in future plant breeding programs to create desirable cultivars.