Use of PCR-RAPD marker to evaluate hybridization between rainbow trout Oncorhynchus mykiss and the caspian trout Salmo trutta caspius

Hybridization is one of the traditional methods of breeding in agriculture and aquaculture as well. Hybrid nature detection has a key role in this valuable breeding plan. For this purpose, we can use different methods; molecular method is one of the most important and pioneering methods for hybrid detection. In this research the possibility of RAPD marker to evaluate hybridization between female rainbow trout Oncorhynchus mykiss and male Caspian trout Salmo trutta caspius was analyzed. For this purpose 30 RAPD primers were randomly selected and parent's genotypes were analyzed through PCR. Only 2 out of 30 primers including H-09 5´TGT AGC TGG G3´ and P-12 5´AAG GGC GAG T 3´ showed remarkable polymorphism and clear specific bands between at least one pair of broodfish. All (except one) F1 larvae inherited total specific bands of two parents which completely confirmed the accuracy of hybridization between two species. In this research the efficiency of RAPD marker to evaluate hybridization between two commercial salmonid species in Iran was documented.