|تاریخ چاپ: 1399/09/08|
|The effect of Migri-Heal® on nitric oxide production in an in vitro inflammatory model of primary microglial cells|
|Author(s):||Mahmoud Hassani، Farzaneh Sabouni، Solaleh Emamgholipour، Mohammad Hessam Rafiee، Mohammad-Sadegh Fallah، Shah-Sanam Abbasi، Mohammad Ansari|
BackgroundRecently, much attention has been directed towards considering activated microgelial cells as putative targets for treatment of neurological disorders. MigriHeal® as a novel herbal remedy was introduced for the treatment of migraine headaches. The previous researches has shown that MigriHeal® extracts can decrease NO in an in vitro inflammatory model. The aim of this study was to investigate the effect of MigriHeal® on NO generation from LPS- stimulated microglia cells.
Materials And MethodsNeonatal rat primary microglial cells were isolated from the mixed glial cultures and the purity of the cultures was determined by immunocytochemistry. Microglial cells were pretreated with Migri-Heal® and activated by 1μg/ml LPS. Subsequently, NO levels in the culture supernatants were measured by a griess reaction. Our results showed that Migri-Heal® 50μg/ml significantly reduced NO level in inflamed microglia in a dose-dependent manner.
ResultsThe results showed that different concentrations of Migri-Heal® had no prominent effect on cell viability in presence of LPS as compared with the control group. In addition, the pretreatment of microglia cells with Migri-Heal® can prevent from a morphological changes of the cells into the round and phagocytic shape.
ConclusionOur study demonstrated that MigriHeal® might have NO scavenging properties. Integrative studies are warranted to uncover the novel pharmacological insights of this herbal remedy as an putative therapeutic approach against diseases - associated with inflammation.
|Keywords:||inflammation، microglia، Migri-Heal®، nitric oxide|
|Published:||Archives of Medical Laboratory Sciences, Volume:2 Issue: 2, 2016|
|Full text:||PDF is available on the website.|