|تاریخ چاپ: 1399/04/12|
|Neonatal Rat; A Suitable Animal Model for Experimental Cryptosporidiosis|
|Author(s):||Seyede Manizhe Heidarnejadi، Abdollah Rafiei *، Manoochehr Makvandi، Majid Pirestani، Jasem Saki، Ataallah Ghadiri|
BackgroundCryptosporidiosis is a major public health problem for neonatal livestock worldwide. Cryptosporidium parvum infects intestinal epithelial cells via contaminated food or drinking water and leads to cryptosporidiosis. Most of the animal model studies on infectivity of C. parvumare conducted on the neonatal mice.
ObjectivesThe current study aimed at evaluating the infectivity of C. parvum in neonatal rat as an animal model.
MethodsA dose of 100,000 to 120,000 C. parvum oocysts (Iowa strain, BTF Company, Sydney, Australia) was orally inoculated in a group of 30 neonatal Wistar rats aged 2 days old. Eight days postinfection, jejunum, ileum, cecum, colon, and rectum were removed and contents were homogenized and purified using sucrose gradient method.
ResultsOur results indicated that 6 to 12 million C. parvum was found per rat
ConclusionsAnalysis of the study results revealed that the neonatal rat could be used as an alternative animal model to investigate C. parvum.
|Keywords:||Iowa Strain، Purification، Neonatal Rat، Cryptosporidium parvum|
|Published:||Jundishapur Journal of Microbiology, Volume:10 Issue: 7, 2017|
|Full text:||PDF is available on the website.|