|تاریخ چاپ: 1399/04/17|
|Neuroprotective effect of dimethyl fumarate in stroke: The role of nuclear factor erythroid 2-related factor 2|
|Author(s):||Anahid Safari، Hamzeh Badeli Sarkala، Mohammad Reza Namavar، Elias Kargar Abarghoue، Neda Anssari، Sadegh Izadi، Afshin Borhani Haghighi|
There is evidence that supports the neuroprotective effects of dimethyl fumarate (DMF) in stroke. Nuclear factor erythroid 2-related factor 2 (Nrf2) has both anti-oxidant and anti-inflammatory mechanisms. We investigated the neuroprotective effects of DMF via Nrf2 activation in the cortex, striatum, and diencephalon in a middle cerebral artery occlusion (MCAO) model of stroke.
22 Sprague-Dawley male rats were randomized into 3 groups. In DMF-treated group (n = 8), rats received 15 mg/kg oral DMF twice daily by gavage from day 0 to 14 after a 60-minute MCAO. The vehicle group (n = 7) underwent MCAO and were given methocel/H2O, using the same method and schedule. In the sham group (n = 7), neck was opened, but neither middle cerebral artery (MCA) was occluded nor any drug was administered. After 14 days, the animals were sacrificed. The infarct volume were assessed by stereology method. Nrf2 expression was evaluated in the cortex, striatum, and diencephalon by immunohistochemistry method.
Ratio of infarct to total brain volume was significantly lower in the DMF-treated group (5.76%) in comparison with the vehicle group (22.39%) (P < 0.0001). Nrf2 expression was higher in DMF-treated group in comparison with both the vehicle and sham groups in cortex, striatum, diencephalon, and total brain (P < 0.0001). In the DMF-treated group, significant negative correlation between Nrf2 expression and infarct volume was observed in cortex, striatum, diencephalon, and total brain.
DMF induces Nrf2 expression and its neuroprotective effects in different brain anatomical regions.
|Keywords:||Stroke، Dimethyl Fumarate، Middle Cerebral Artery، Nuclear Factor Erythroid 2-Related Factor 2|
|Article Type:||Research/Original Article|
|Published:||Current Journal of Neurology, Volume:18 Issue: 64, 2019|
|Full text:||PDF is available on the website.|