|تاریخ چاپ: 1399/07/08|
|Effect of High-Intensity Interval Training on Apoptotic Gene Expression in Rat Myocardial Tissue|
|Author(s):||assan Pourrazi *، Masoud Asgharpour Arshad، Farhad Gholami، Soheila Abbasi|
Excessive apoptosis in the myocardium contributes to the pathogenesis of heart disease. Evidence indicates that high-intensity interval training (HIIT) can alter myocardial apoptosis-related signaling.
The purpose of the study was to investigate the effect of 12 weeks of HIIT on some indices of apoptosis, including Bcl-2, Bax, and caspase-3 in the myocardial tissue of male rats.
Thirty male Wistar rats (age range: 8 - 10 weeks) were randomized into three groups, including sham (n = 10), control (n = 10), and HIIT (n = 10). The experimental group underwent HIIT consisting of 2 - 8 repetitions of 4-min high-intensity intervals (85% - 90% peak speed) interspersed with low-intensity intervals (45% - 50% peak speed), performed five times/week over 12 weeks. Forty-eight hours after the last training session, animals’ hearts were removed. The gene expressions of Bcl-2, Bax, and caspase-3 were analyzed by the RT-PCR method. The independent t-test was used for statistical analysis (P < 0.05).
The gene expressions of Bcl-2 and Bcl-2/Bax ratio were higher in the training group than in the control group (P = 0.001 and P = 0.002, respectively). Regarding Bax and caspase-3 gene expression, although the mean levels were lower in the exercise group than in the control group, the differences were statistically insignificant (P = 0.57 and P = 0.27, respectively).
Overall, it seems that HIIT significantly increases the expression of anti-apoptotic indicator Bcl-2 and the ratio of Bcl-2/Bax in the myocardium of male rats. However, further studies are needed to show the effects of HIIT on myocardial apoptosis.
|Keywords:||Apoptosis، Myocardium، High-Intensity Interval Training، Apoptotic Genes|
|Article Type:||Research/Original Article|
|Published:||Gene, Cell and Tissue, Volume:7 Issue: 2, 2020|
|Full text:||PDF is available on the website.|