This research was conducted to determine the mediating role of accepting gender roles in the relationship between character strengths and happiness in women. This descriptive-correlation research was of structural equation modeling type. The statistical population of this study was female students in Tehran in 2021; 300 people participated in this study by available sampling method from 5 universities. Data collection using the Attitude Toward Gender Role Questionnaire (ATGRQ; Kiani et al., 2008), The Values in Action Inventory of Strengths (VIA-IS; Peterson and Seligman, 2004) and Oxford Happiness Questionnaire (OHQ; Argyle, 1989) was done. The results showed that the proposed model has a good fit; Also, the results showed that character strengths and acceptance of gender roles had a significant direct effect on happiness (P<0.05); Also, character strengths had a positive effect on the acceptance of gender roles (P<0.05). Another result of the bootstrap test showed that acceptance of gender roles had a significant mediating role between character strengths and happiness (P<0.05). The results of this study indicate that character strengths have a direct effect on women's happiness through the mediation of accepting gender roles.