فهرست مطالب

Avicenna Journal of Phytomedicine - Volume:7 Issue:2, 2017
  • Volume:7 Issue:2, 2017
  • تاریخ انتشار: 1395/12/23
  • تعداد عناوین: 11
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  • Jatuporn Noosud, Narissara Lailerd, Autchara Kayan, Chaiwat Boonkaewwan* Pages 101-106
    Objective
    Stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity. The aim of the present study was to examine in vitro and in vivo effects of stevioside on rats plasma levels of tumor necrosis factor- α (TNF-α), interleukin-1β (IL-1β), TNF-α and IL-1β release from lipopolysaccharide(LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs).
    Materials And Methods
    Male wistar rats weighing between 170-220 g were given stevioside (0, 500 and 1000 mg/kg BW/day) for 6 weeks. Mononuclear cells were separated from peripheral blood samples. TNF-α and IL-1β levels in plasma and the release of TNF-α and IL-1β from PBMCs were determined using rat enzyme-linked immunosorbent assay (ELISA) kits.
    Results
    Plasma levels of TNF-α and IL-1β were found to be non-detectable in control and groups treated with 500 and 1000 mg/kg of stevioside. Regarding TNF-α release from LPS-stimulated PBMCs, rats that were orally fed with 500 and 1000 mg/kg of stevioside were significantly different (p
    Conclusion
    Consumption of stevioside has an inhibitory effect on the release of TNF-α and IL-1β from LPS-stimulated PBMCs in rats.
    Keywords: Stevioside, TNF- α IL-1β Peripheral blood mononuclear cells
  • Fatemh Atarzadeh, Amir Jaladat, Ladan Dastgheib, Gholamreza Amin, Majid Nimrouzi, Mohammad Kamalinejad* Pages 107-115
    Objective
    Pemphigus is a rare autoimmune disease that may be fatal without proper medical intervention. It is a blistering disease that involves both the skin and mucus membranes, in which the most important causes of death comprise superimposed opportunistic infections and complications of long-term high-dose corticosteroid therapy or prolonged consumption of immune suppressant drugs. Skin lesions are the most important sources of infection, and any local treatment decreasing the healing time of lesions and reducing the total dosage of drugs is favorable.
    Materials And Methods
    Here, we review the probable mechanism of action of a traditional formulary of Cassia fistula (C. fistula) fruit extract in almond oil as a new topical medication for reducing the duration of treatment of pemphigus vulgaris erosions.
    Result
    C. fistula fruit oil has lupeol, anthraquinone compounds as rhein and flavonoids.
    Previous in vitro and animal studies on C. fistula fruit have demonstrated wound healing, antioxidative, anti-leukotrienes, anti-inflammatory, antibacterial and antifungal effects of this plant.
    Conclusion
    It is hypothesized that C. fistula L. can be a botanical therapeutic choice for treatment of pemphigus erosions.
    Keywords: Pemphigus vulgaris, Cassia fistula L, Traditional Persian Medicine (TPM), Topical therapy
  • Mojtaba Pourzaki, Mansour Homayoun, Saeed Sadeghi, Masoumeh Seghatoleslam*, Mahmoud Hosseini, Alireza Ebrahimzadeh Bideskan Pages 116-128
    Objective
    Coriandrum sativum (C. sativum) as a medicinal plant has been pointed to have analgesic, hypnotic and anti-oxidant effects. In the current study, a possible preventive effect of the hydro-alcoholic extract of the plant on neuronal damages was examined in pentylenetetrazole (PTZ) rat model of seizure.
    Materials And Methods
    Forty male rats were divided into five main groups and treated by (1) saline, (2) PTZ: 100 mg/kg PTZ (i.p) and (3-5) 50, 100 and 200 mg/kg of hydro-alcoholic extract of C. sativum during seven consecutive days before PTZ injection. After electrocorticography (ECoG), the brains were removed to use for histological examination.
    Results
    All doses of the extract reduced duration, frequency and amplitude of the burst discharges while prolonged the latency of the seizure attacks (p
    Conclusion
    The results of this study allow us to conclude that C. sativum,because of its antioxidant properties, prevents from neuronal damages in PTZ rat model of seizure.
    Keywords: Coriandrum sativum, Seizure, Apoptosis, Neuronal damage, Hippocampus
  • Mansoure Masoudi, Mahmoud Kopaei Rafieian, Sepide Miraj * Pages 129-136
    Objective
    Due to the high incidence of bacterial vaginosis (BV) and its resistance to chemical medications and considering the anti-bacterial and anti-fungal effects of Myrtus communis, the present study aimed to compare the therapeutic effects of the vaginal gel of M. communis 2% (in metronidazole base) with metronidazole vaginal gel 0.75% alone on BV.
    Materials And Methods
    This research was a random­ized controlled clinical trial conducted on 80 women of 18-40 years old with BV. Patients were divided into two groups of 40 women. Diagnostic criteria were Amsel's criteria and Gram staining. The first group received vaginal gel of metronidazole plus M. communis 2% and the second group received metronidazole vaginal gel alone for five consecutive nights. Therapeutic effects and Amsel’s criteria were assessed after one week. Finally, the data were analyzed by SPSS 16 using t-test and Chi square tests.
    Results
    There was a significant difference in the therapeutic response between the two groups. The results demonstrated that the combination of metronidazole and M. communis had a higher efficiency (p
    Conclusion
    Findings of the study suggested that adding M. communis extract to metronidazole increases the efficiency of BV treatment.
    Keywords: Myrtus communis, metronidazole, Bacterial vaginosis
  • Azar Hosseini, Shima Saeidi Javadi, Azar Fani Pakdel, Seyed Hadi Mousavi * Pages 137-144
    Objective
    Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima (K. odoratissima) extract associated with radiotherapy in cervical cancer cells (HeLa cell line).
    Materials And Methods
    Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.
    Result
    K. odoratissima decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in K. odoratissima-induced toxicity. It was also shown that K. odoratissima sensitizes cells to radiation-induced toxicity.
    Conclusion
    Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.
    Keywords: HeLa cell line, Kelussia odoratissima, Cytotoxicity, Radiotherapy, Apoptosis
  • Samaneh Rahamoz Haghighi, Malek Hossein Asadi *, Hassan Akrami, Amin Baghizadeh Pages 145-156
    Objective
    Acorus calamus (A. calamus) has been used as a medicinal plant in Asia for its effects on digestive system for the last 2000 years. To investigate the anti-cancer activity of rhizome of A. calamus, the ethanolic and methanolic extracts and essential oil of the rhizome were prepared and their effects were assessed on human gastric cancer cell line (AGS).
    Materials And Methods
    The viability of cells which were treated with the extracts and the essential oil was assessed by MTT assay. To evaluate the anti-angiogenic property of the extracts, in vitro tube formation assay was done. Cell cycle distribution and the expression of Oct4 and Nucleostemin, after treatments, were checked by flowcytometry and quantitative RT-PCR, respectively. Furthermore, analysis of essential oil from A.calamus was done by GC-MS.
    Results
    Our results showed that the growth of AGS cells was inhibited by the extracts and essential oil and the extracts inhibited the angiogenesis in HUVEC cells. Our data revealed that the extracts and essential oil of A. calamus caused G1 arrest in AGS cells and downregulation of Oct4 and NS after treatment. By GC-MS analysis, we found new compoundssuch as epiprezizaene, valencene and isocyclocitral in essential oil of A.
    Conclusions
    All together, our results showed that the extracts of A. calamus have anti-proliferative and anti-angiogenic effects on cancer cells.
    Keywords: Acorus calamus, Gastric cancer, Anti, proliferative, Angiogenesis, Oct4, Nucleostemin
  • Maryam Varasteh Kojourian, Parvaneh Abrishamchi *, Maryam Matin, Javad Asili, Hamid Ejtehadi, Fatemeh Khosravitabar Pages 157-168
    Objective
    Achillea is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for Achillea pharmacological effects. We aimed to determine phenol and flavonoid contents, besides antioxidant activities of different extracts from Achillea eriophoraa (A. eriophora) DC. and Achillea biebersteinii (A. biebersteinii) Afan. (endemic species in Iran) and to investigate their effects on human cells.
    Materials And Methods
    Achillea extracts, were prepared by maceration and shaking methods, from different parts (aerial parts, stem, leaves and inflorescence) of two species using methanol and ethanol as solvents. Total phenol and flavonoid contents were measured by spectrophotometry, and antioxidant activities of the extracts were determined by DPPH radical scavenging, BCB and TBARS assays. Cytotoxicity and antioxidant activities of the extracts were investigated in Human Foreskin Fibroblast (HFF3) cells using MTT, comet and H2O2 assays.
    Results
    Methanol extracts of A. biebersteinii prepared from leaves and inflorescence by maceration method exhibited maximum phenol (1657.58 ± 36.45 mg GAE/100 g DW) and flavonoid (264.00 ± 62.16 mg QUE/100 g DW) contents. Leaf methanol extract showed significantly higher antioxidant activity (0.0276 ± 0.003, 0.16 ± 0.016 and 13.96 ± 0.26 mg/ml for DPPH, BCB and TBARS IC50s, respectively) than those of the other extracts. Leaf extract of A. biebersteinii was not cytotoxic even at the highest examined dose (512 µg/ml) and inhibited cell toxicity induced by H2O2 (98% viability for the cells pretreated with plant extract in the presence of H2O2). Comet assay also confirmed high DNA protective activity of leaf extracts.
    Conclusion
    Achillea extracts possess remarkable antioxidant activity, and could be good natural alternatives to synthetic antioxidants in pharmaceutical and food industries.
    Keywords: Extraction, Flavonoids, DPPH, MTT assay, Comet, HFF3 cells
  • Akram Ahangarpour, Hamid Heidari *, Ali Akbar Oroojan, Farhang Mirzavandi, Khalil Nasr Esfehani, Zienab Dehghan Mohammadi Pages 169-179
    Objective
    Arctium lappa (burdock), (A. lappa) root has hypoglycemic and antioxidative effects, and has been used for treatment of diabetes in tradition medicine. This study was conducted to evaluate the antidiabetic and hypolipidemic properties of A. lappa root extract on nicotinamide-streptozotocin (NA-STZ)-induced type2 diabetes in mice.
    Materials And Methods
    In this investigation, 70 adult male NMRI mice (30-35g) randomly divided into 7 groups (n=10) as follow: 1-control, 2-type 2 diabetic mice, 3-diabetic mice that received glibenclamide (0.25 mg/kg) as an anti-diabetic drug, 4, 5, 6 and 7- diabetic and normal animals that were pre-treated with 200 and 300 mg/kg A. lappa root extract, respectively, for 28 days. Diabetes has been induced by intraperitoneal injection of NA and STZ. Finally, the blood sample was taken and insulin, glucose, SGOT, SGPT, alkaline phosphatase, leptin and lipid levels was evaluated.
    Results
    Induction of diabetes decreased the level of insulin, leptin and high density lipoprotein (HDL) and increased the level of other lipids, glucose, and hepatic enzymes significantly (p
    Conclusion
    The results showed that A. lappa root extract, at specific doses, has an anti-diabetic effect through its hypolipidemic and insulinotropic properties. Hence, this plant extract may be beneficial in the treatment of diabetes.
    Keywords: Arctium Lappa, Type 2 diabetes, Insulin, Lipid profile, Hepatic enzyme
  • Alireza Golshan, Parichehr Hayatdavoudi, Mousa Al Reza Hadjzadeh *, Abolfazl Khajavi Rad, Nema Mohamadian Roshan, Abbasali Abbasnezhad, Seyed Mojtaba Mousavi, Roghayeh Pakdel, Batool Zarei, Azita Aghaee Pages 180-190
    Objectives
    The antioxidant capacity impairs in kidney and urinary bladder of animals with stone disease. Herbal medicine can improve the antioxidant condition of renal tissue. Cynodon dactylon (C. dactylon) is a medicinal plant with antioxidative and diuretic properties and different preparations of this plant have shown promising effects in stone disease. Assessment of the whole plant decoction to prevent kidney stone disease as well as its antioxidant effects was the aim of this paper.
    Materials And Methods
    Fifty male Wistar rats were randomly divided into 5 experimental groups (n=10). One group was left without treatment and four groups received ethylene glycol (1% v/v) in drinking water for 6 weeks. Three doses of Cynodon dactylon aqueous decoction (12.5, 50 and 200 mg/kg BW) were added to the drinking water of groups 3-5. Finally, water intake, 24-hour urine volume, MDA, total thiol concentration and FRAP value were measured in the serum and kidney tissues. The CaOx depositions were evaluated by hematoxylin and eosin staining.
    Results
    Compared to the ethylene glycol-treated group, 200 mg/kg C. dactylon, lowered stone incidents, decreased urine volume, increased FRAP/g Cr (43%) and thiol content (p
    Conclusion
    A minimum dose of 200 mg/kg C. dactylon reduced stone formation and simultaneously increased total antioxidant power of serum and preserved MDA content and water.
    Keywords: Cynodon dactylon decoction, FRAP, MDA, Nephrolithiasis, Antioxidant, Ethylene glycol
  • Mohamed Eddouks *, Ahmed Lemhadri, Morad Hebi, Ahmed El Hidani, Naoufel Ali Zeggwagh, Bachir El Bouhali, Lhoussaine Hajji, Remy Burcelin Pages 191-198
    Objective
    As the aqueous extract of Capparis spinosa (CS) possess antidiabetic effect, he present study aims to reveal the possible mechanism of action of CS in diabetic mice.
    Materials And Methods
    Both single and repeated oral administrations of aqueous extract of CS were performed in multi-low dose streptozotocin-induced (MLDS) diabetic mice. Euglycemic hyperinsulinemic clamp was used in association with the endogenous glucose production (perfusion rate of 3-3H glucose) to evaluate the effect of CS aqueous extract on insulin sensitivity.
    Results
    Our study showed that aqueous extract of CS possess a potent hypoglycaemic activity in MLDS diabetic mice. Furthermore, the analysis perfusion of 3-3H glucose demonstrated the parallel decrease of basal endogenous glucose production (EGP) with the hypoglycaemic activity. EGP was lower in CS-Treated group when compared to the control group (p
    Conclusion
    We conclude that the antihyperglycemic effet CS is probably due to the inhibition of basal endogenous glucose production and the improvement of insulin sensitivity in MLDS diabetic mice.
    Keywords: Diabetes, Streptozotocin, Insulin sensitivity, Mice