مجله علوم پزشکی پارس
سال چهاردهم شماره 4 (پیاپی 38، زمستان 1395)

Background and Surfactant injection through Intubation-SURfactant-Rapid Extubation (INSURE) and conventional mechanical ventilation are methods that used for treatment of respiratory distress syndrome in preterm infants. The aim of this study was to compare the short term prematurity complications in neonates who were treated with one of these two methods.
Material and This descriptive study that performed on documents of 120 preterm neonates who hospitalized in NICU and received surfactant at 91-92 solar years.Neonates who had inclusion criteria formed two groups based on non randomized sampling and conventional treatment in the same year in this hospital that include: Intubation-SURfactant-Rapid Extubation group and Mechanical Ventilation group in years of 1392 and 1391 respectively. Required variables extracted from files and the questionnaire was completed. Data were analyzed by SPSS version21 through t-test and chi-square test with95% confidence interval.
The results showed that intraventricular hemorrhage, death and disseminated intravascular coagulation (p=0/01) and retinopathy of prematurity (p=0/03), Pneumothorax (p=0/001) in the INSURE group were significantly less than Mechanical Ventilation group. But, not differed in two groups about the pulmonary hemorrhage (p=0/4), sepsis (p=0/29) and patent ductus arteriosus (p=0/24). The onset of milk and hospital discharge in INSURE group was sooner than MV group but this difference was not statistically significant (p= 0/06, 0/1 respectively).
Based on the findings of this study, INSURE method in preterm infants with respiratory distress related to prematurity complications compared to mechanical ventilation strategy is more safe. Therefore, further studies are suggested in order to use this method for treatment of respiratory distress syndrome in neonatal intensive care units.

Carbapenems are drugs with an extended spectrum but There are some problems when Carbapenems resistance is increased. Pseudomonas aeruginosa is an important nosocomial pathogen but Imipenem resistance increases and it is on second line therapy of multidrug resistance Pseudomonas aeruginosa infections. The importance of Enterobacteriaceae family is due to the increasing number of patients, According to this bacterium family 30-40 percent of Septicemiais infected and 70 percent of urinary tract infections. Carbapenems are the last defense line in critical infections with these pathogens.
this study collected 196 isolated bacteria of Pseudomonas aeruginosa and Enterobacteriaceae family from Hospitals patient of Tehran and evaluates them by phenotypic and biochemical methods. also, The researcher studies the disk diffusion method and use it to determine antibiotic sensitivity of all bacteria according to the CLSI standard table.
From the total of 196 bacteria collected, the resistance to Meropenem is %39.79 and Imipenem is %45.91 and 30.10 percent to Meropenem and Imipenem resistance so that the percent resistance of bacteria is as follows: Pseudomonas aeruginosa resistance to Meropenem is %39.62 and Imipenem is %43.39. Meropenem and Imipenem resistance in Enterobacteriaceae family are as follows: Salmonella resistance to Imipenem and Meropenem is %0 and %50, Citrobacter koseri the resistance to Imipenem and Meropenem %0 and %50, Citrobacter freundii Meropenem resistance of %28.57 and Imipenem %28.57, Escherichia coli resistance to Meropenem %39.70 and Imipenem %47.05, Klebsiella oxytoca resistance to Meropenem %46.6 and %40 Imipenem and Meropenem resistance Klebsiella pneumoniae %34.14 and Imipenem %53.65, respectively.
There is an increasing resistance to Meropenem and Imipenem antibiotics in treatment of Pseudomonas aeruginosa and Enterobacteriaceae family in our country. Hence, it should be properly and reasonably use these antibiotics.

Tuberculosis is a chronic infectious and contagious disease that in according to global burden of disease (DALY) it is in the seventh category and it’s predicted that by 2020 still remains in the same category.The present study was conducted to evaluate the demographic tuberculosis study in the city of Jahrom.
In this descriptive - Analytical study during the years 2005-2014 The records of all patients with TB Checked out .Data were analysed via SPSS software Version 22 using descriptive statistics methods and Chi-Square test and t-student at the significant independent level P Out of 114 TB registered patients 67.5% were male ¡69.3% were infected by pulmonary TB and 6.1% were infected by HIV. Also the patients were Afghanian national, 31.6% and 64% of the patients were Urban. The highest prevalence of TB disease (Pulmonary and Extra -pulmonary) were observed in under 40 years of age category(44.7%) . There was no significant differences in types of TB (Pulmonary and Extra-pulmonary) according to Sex, Nationality, Residing place, Age groups and Occupations (P>.05) ,whereas this differences were significant in terms of exposures with TB cases (P Accordingly with attention to the low age (young age) of the stricken peoples in the present study it seems to achieve the target goals and more success in preventing and fighting the TB disease more attention should consider towards the obstacles of TB treatments and controlling programs implementation, and patients surveillance and training programs for this population groups should be priority and precedency of Jahrom city health centre

Culex spp is the prevalent in many tropical countries, is causing filarial fever among human beings. The easiest way to prevent this disease is to eliminate the vector. In this study, we used the extract of aerial parts of Hyoscyamus niger and Nerium oleander, to destroy the larvae of this vector and the results of these two plants were compared together.
Collected and dried aerial parts of the plant were extracted using methanol, solvent was then removed with a rotary evaporator. Mosquito larvae were collected and identified from stagnant water pits and ponds around the Birjand, Iran; The larvae were then placed on a plate containing a certain concentration of the extract of oleander or henbane separately, and their mortality were recorded and analyzed.
H. niger and N. oleander extract of aerial parts both had a positive effect on the elimination of larvae of the Culex spp. Among the results, the flower extract of henbane was the most powerful extract after 72 hours to eliminate the larvae of Culex spp (LC50= 0/46 ppm) and the stem extract of henbane was the weakest one (LC50= 37/57 ppm).
Due to strong larvicidal effect of the extract of henbane in 72 hours than other extracts, suggesting that the extract of henbane can be used as an organic pesticide to eliminate Culex spp larvae.

Acute exhaustive exercise increase production of reactive oxygen. It may be inefficient antioxidant capacity of the body. Therefore exercise training and antioxidants consumption have been shown to up regulate antioxidant protection. The present study aimed to determine the protective effect of pomegranate peel hydro alcoholic extract supplementation on oxidative responses induced by an exhaustive exercise schedule in rats.
Thirty two male rats were evenly divided into: (1) control: received saline (0.2 ml saline/ rat) by oral administration via epigastric tube.(2) received oral administration of 200 mg/kg Pomegranate peel hydro alcoholic extract.(3) received oral administration of 250 mg/kg Pomegranate peel hydro alcoholic extract. (4) Received oral administration of vitamin E 5 mg/kg. Animals were submitted to swimming exercise for an 8-week. At the end of the experiment, blood samples were collected for serum. samples were analyzed for glutathione peroxidase , catalase activities, levels of Malondialdehyde and glutathione.
Glutathione peroxidase and catalase activities were significantly increases in PPHE200)97.61±5.91)GPX (4.38±0.45)CAT ,PPHE250)105.63±2.88)GPX (3.57±0.24)CAT and vit E )110.01±8.36)GPX (3.6±0.59)CAT than in the control)76.72±4.49)GPX (2.85±0.27)CAT (p In this study, it was shown that Pomegranate peel hydro alcoholic extract supplementation may be beneficial in antioxidant defense system improvement and reduce oxidative stress induced by an exhaustive exercise.

Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy, peptic ulcers and gastric lymphomas in humans. Tpx (thiolperoxidases) is encoded by the tagD gene and plays a significant role in colonize of H. pylori to stomachs. This gene stimulates the immune system in the host cells. The aim of this study was to isolate and cloning of tagD gene in the eukaryotic expression vector PFLAG-CMV-3 as a DNA vaccine candidate.
Material & In this research, tagD gene (537 bp) was amplified by PCR. The PCR products were cloned by using cloning kits of Thermo Fisher Company in pTZ vector. This gene was Subcloned in the eukaryotic expression vector (PFLAG-CMV-3 vector), then transferred into CHO cells by electroporation method and finally was expressed.
The results indicate that the gene amplification and cloning of tagD gene, was successful, and the pTZ-tagD vector was formed. PFLAG-CMV-3 Vector construction was confirmed by digestion and gene sequencing. The 19 kDa band was observed by gene expression analysis on SDS-PAGE.
tagD gene in the PFLAG-CMV-3-tagD recombinant vector has an ability to produce specific protein in CHO cells. Therefore, this gene construct is useful to evaluate the immunogenicity as a DNA vaccine against of the Helicobacter pylori infection in human.

Background and Based on Janda’s theory of kinetic chain, dysfunction in one motor segment can affect other segments in the body. The purpose of the study was to determine the correlation between Low Back Pain (LBP) and foot overpronation in patients referred to Hazrat Rasool Hospital.
In this cross sectional study, 242 subjects were classified into two groups, LBP and healthy groups and evaluated for the duration and intensity of LBP, flexibility of trunk, foot overpronation (by Helbing sign and Navicular Drop test ) and ankle dorsiflexion range. The Independent sample t- test and Chi-Square test were conducted for statistical analysis of the data.
A significant correlation between foot overpronation and LBP (p=0.001) was found in the study. There were significant relations between LBP intensity and foot overpronation (p=0.001); between the history of LBP and foot overpronation (p=0.001) and also on the between foot overpronation and ankle dorsiflexion range (p=0.014).
The findings of this study indicate significant relations between the incidence, intensity and duration of LBP in patients with foot overpronation; also, the ankle dorsiflexion range was reduced in the subjects with foot overpronation.

Propofl is an anesthetic drug which has shown many other effects in different studies. The purpose of this study has been evaluation of non-anesthetic properties of propofol in skin flap survival in rats.
Thirty two adult female white rats were randomly divided into two groups. In Group 1, anesthesia with ketamine (40mg/kg) and group 2 anesthesia with propofol (100 mg / kg) was performed intraperitoneally (IP). The skin on the back of rats (2.5 × 8 × 8 cm) was prepared aseptically. Full-thickness skin was removed in three parts and returned to the original location immediately. The skin was sutured with single interrupted pattern and nylon 0/5. Survival rate of the skin flaps were evaluated daily. Histopathological evaluation by a blind pathologist at 1, 4, 7 and 21 days was done. Interleukin 1 and 6 in blood samples before and after creation of flaps was measured at 12, 24 and 168 hours following euthanasia.
Flap survival rate at day 21 and IL-6 at day 0, 12 hour and day 1 in propofol group were significantly higher than ketamine group and IL-1 was not significantly different. The amount of collagen deposition at days 1 and 7, epithelialization at days 1 and 4, fibroblast proliferation at day 21 and vascularity at days 4, 7 and 21 in propofol group were significantly more than ketamine, but the inflammatory cells infiltration at day 7 in propofol group was less than ketamine. Pathological signs of healing were more prominent in propofol group.
It is concluded that propofol has more restorative effect on skin flap survival in comparison with ketamine, but its definite confirmation needs more precisely researches.