فهرست مطالب

Biological and Biomedical Journal - Volume:2 Issue: 1, Winter 2016
  • Volume:2 Issue: 1, Winter 2016
  • تاریخ انتشار: 1395/04/23
  • تعداد عناوین: 6
  • Sadegh Fattahi, Monireh Golpour, Haleh Akhavan Niaki* Pages 1-10
    Urtica dioica is a perennial plant used as herbal medicine due to its many pharmacological and clinical effects. Because of its antioxidant activity, it is widely used in traditional diabetes treatment but is also known as antimicrobial, anti inflammatory or anti prostate cancer agent. Extensive studies have been conducted on different parts of this plant and their biological effects. Here we reviewed the effect of different parts of this plant including leave, seed, root and aerial part extracted with various methods in treatment of diseases. Various beneficial effects were reported on animal models without apparent side effects, which led us to consider it as an emerald to be more deeply discovered in the kingdom of health.
    Keywords: Urtica dioica, diabetes, cancer, antioxidant, anti-inflammatory
  • Kenniyan Kumar Srichinthu, Thukanayakanpalayam Ragunathan Yoithapprabhunath, Ravi Teja Chitturi*, Andamuthu Yamunadevi, Aparna Devi Potsangbam, Deepak Ningombam Singh Pages 11-20
    Fibro osseous lesions (FOLs) are a generic designation of poorly defined group of lesions which are recognized to affect the jaws and the craniofacial bones which are known for their confusing area in diagnostic pathology. The disease comprises of varied processes in which the normal architecture of bone is replaced by fibrous tissue containing varying amount of foci of mineralization. Regardless of the advancement in molecular analyzing techniques, the classification, diagnosis, and management of FOLs remain confusing due to multiple histological and radiographic similarities. Even though the histopathology for all FOLs is similar, their behavior ranges widely from dysplasia, hamartoma to benign neoplasia with occasional recurrence and hence radiology plays a central role in their diagnosis. The article throws a light on the various classification systems given for FOLs by various authors at different times due to expansion in molecular studies and also highlights the role of radiographic and other imaging techniques in the diagnosis of FOLs, which will enable us to adopt a uniform terminology and to aid the surgical pathologist in the diagnosis of this diverse group of maxillofacial lesions.
    Keywords: Fibro osseous lesions, pathophysiology, radiography
  • Zeynab Dadgar, Nargess Abdali, Abolfazl Elyasi Irai, Zeinab Salehian* Pages 21-30
    This investigation aimed to examine the protective function of vitamin E on rat bone marrow mesenchymal stem cells (MSCs) treated with cadmium chloride. Rat bone marrow MSCs were extracted using flashing-out and cultured in DMEM containing 10% FBS and 100 U/ml Pen/Strep. At the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride vitamin E and vitamin E, for a period of 5, 10, 15 and 21 days in the osteogenic media. The cell viability, bone matrix mineralization and intra cellular calcium were measured using MTT assay, alizarin red staining, von kossa staining and calcium kit, respectively. Alkaline phosphatase activity was also estimated. Morphology and DNA cleavage were studied with the help of fluorescent dye and comet assay. Data were analyzed using one way ANOVA. The viability and bone matrix mineralization of the cells treated with cadmium chloride was reduced significantly in comparison with the control group. Chromatin condensation, reduction of nuclei diameter and cytoplasm shrinkage were observed in cadmium group. The intracellular calcium and alkaline phosphatase activity of the cells decreased significantly with cadmium when compared to control group. A significant increase of these parameters was found in the group of cadmium chloride vitamin E compared to the control ones. Results show that vitamin E exhibits protective role against the toxic influence of cadmium on all studied parameters in rat bone marrow MSCs.
    Keywords: Bone marrow_mesenchymal stem cells_Vitamin E cadmium_toxicity_rat
  • Shermineh Heydari, Elaheh Ghods, Majid Mojarrad, Zahra Mozaheb, Ehsan Ghayoor Karimiani, Mohammad Hassanzadeh Nazarabadi* Pages 31-38
    Myelodysplastic syndrome (MDS), is a group of heterogeneous disorders of hematopoietic stem cell colonies which is determined by incomplete hematopoiesis in one or more cell lines. The incidence increases with age and it has less been reported among patients under 50 years of age. The commonest form of MDS is sporadic, and familial occurrence of MDS is rare. Patients with familial MDS are younger at the time of diagnosis than individuals with sporadic disease and are faced with an unusual family history of more than one first-degree relative with MDS. In this study, we investigated a family with 16 members which has a family history of MDS in three consecutive generations. Cytogenetic and chromosome analyzes were performed on the 16 members of the family. Moreover, let-7a miRNA expression and its target gene, NRAS were evaluated compared to the control group. The karyotype analysis in this family shows two patients with abnormal karyotype, whereas expression of the let-7a and NRAS in this family showed a significant decrease compared to the control group. Regarding the decreased expression of let-7a in patient samples, this miRNA can be further considered as a marker for early diagnosis of MDS disease.
    Keywords: Myelodysplastic syndrome, Familial MDS, Cytogenetic, let-7a, NRAS
  • Mohammad Ali Bayani, Ahmad Karkhah, Seyed Reza Hoseini*, Roya Qarouei, Haji Qorban Nourodini, Ali Bijani, Robert G. Cumming Pages 39-46
    Diabetes mellitus (DM) and osteoporosis are common diseases and their prevalence increases with age. Several investigations have indicated that type 1 DM has a significant relationship with bone loss, whereas in type 2 diabetes, this relationship is controversial. Therefore, this study was conducted to determine the relationship between osteoporosis and type 2 DM in elderly people. This population-based study had been carried out on 1151 elderly people in Amirkola, northern Iran. L2-L4 lumbar spine bone mass and the left femoral neck density were measured by dual-energy X-ray absorptiometry (DEXA). In addition, diagnosis of diabetes was done by measuring fasting blood sugar (twice times FBS ≥ 126 mg/dl), according to the WHO criteria or self-reported as well as based on a doctor's prescription. Of total, 362 (31.45%) of patients had DM. The average age of diabetic patients was 68.9± 6.93 years and in non-diabetic group was 68.68± 7.09 years (P= 0.18). The mean L2-L4 lumbar spine bone mass in the diabetic group was 0.90± 0.19 g/cm2 and in the non-diabetic group was 0.85± 0.18 g/cm2 (P= 0.001). The mean lumbar bone mineral density was higher (P= 0.0001) in diabetic men than in non-diabetic men, as well as in women (P= 0.0001). In addition, the mean femoral neck density in diabetic group was 0.85± 0.16 g/cm2 and in the non-diabetic group was 0.84± 0.15 g/cm2 (P= 0.48). Moreover, the femoral neck bone mineral density in diabetic men was higher than in non-diabetics (P= 0.03), whereas in diabetic and non-diabetic women, there was no significant difference (P= 0.52). Our results demonstrated that the mean lumbar and femur bone mineral densities in older people with type 2 DM was higher than people without DM.
    Keywords: Type 2 diabetes mellitus_osteoporosis_bone mineral density
  • Galia Amirbozorgi*, Hamidreza Samadlouie, Seyed Ahmad Shahidi Pages 47-52
    The lactic acid bacteria (LAB) are an industrially important group of probiotic organisms that play an important role in human health by inhibiting harmful and pathogen bacteria growth, boosting immune function, and increasing resistance to infection. This study aimed to isolate, identify, and biologically characterize probiotic LAB strains from Iranian traditional dairy products. A total of 20 samples of traditional fermented milk were collected from six villages of Mazandaran province, Iran. LAB strains were identified according to phenotypic characterization and 16S rRNA gene sequence analysis. All isolates belonged to the Lactobacillus strains. According to the 16S rRNA gene sequences the isolated bacteria were divided into two sub-groups Lactococcus lactis helveticus and Lactobacillus brevis. The dominant isolated Bacillus genus was Lactobacillus helveticus (76.92% of lactobacilli population). The results of the present study showed slight diversity of LAB among traditional dairy products in Mazandaran province, Iran. These bacteria can be introduced as valuable sources for further starter selection.
    Keywords: Probiotic, lactic acid bacteria, 16S rRNA