Caspian Journal of Reproductive Medicine
Volume:3 Issue: 1, Winter-Spring 2017

A number of reports have shown that mesenchymal stem cells (MSCs) from various sources, expressed a number of germ cell (GC)-specific genes innately but there wasn’t any complete and quantitative study. So that, in this study we determined and compared the expression levels of some famous GC-specific genes in human male bone marrow (BM)- and peripheral blood (PB)-MSCs.
Mehtods: Human BM- and PB-MSCs were isolated using a density gradient factor and centrifuging. Then, Passage 3 of both cell types were characterized by flow cytometric analysis and differentiation test and then evaluated by real time RT-PCR for the expression levels of GC-specific genes.

Both of the cell types obviously expressed c-kit, Dazl, Fragilis, Itgb1, Nanos3, Oct4 and Blimp1 at high levels and except Itgb1 which was expressed at a higher level in PB-MSCs than in BM-MSCs (p

Overall, it could be said that BM- and PB-MSCs have a little bit different GC-specific gene expression pattern. Although, these data are very important, so many detailed and creative research studies still needed in order to find the way of using MSCs in cell-based therapies of infertility.

The primary infection of pregnant women by Toxoplasma gondii can cause severe complications in the fetus, and can also lead to neurological complications. The aim of the present study was to determine the seroprevalence and seroconversion rates of Toxoplasma gondii in female students who were at childbearing age in Iran.
This cross sectional study was performed on female students at Babol University of Medical Sciences (Babol, Iran) over a three-year period between 2012 and 2014. Two ml blood sample was obtained from volunteers, and the specific antibodies (IgG) to T. gondii were detected by Enzyme-Linked Immunosorbent Assay. Second, the blood samples obtained from seronegative cases were used to evaluate the seroconversion during a year.
The mean age of the subjects was 21±2.2, ranging from18 to 35 years. Overall, 28% of the participants were positive and 232 (72%) were negative for anti-Toxoplasma antibodies (IgG). The rate of this infection was higher among female students from Mazandaran province (31.7% vs 12.5%) (P=0.031). Based on the results of Enzyme-Linked Immunosorbent Assay, the seroconversion rate of Toxoplasma infection among female students was estimated to be 2.5%.
This study found a noticeable rate of seroconversion in female students. Therefore, there is an urgent need for a national screening project to determine the seroconversion rate of Toxoplasma infection in women at childbearing age, particularly pregnant women from all over Iran.

Studies have revealed that Polycystic Ovary Syndrome (PCOS) usually begins in adolescence and it might manifest differently in different populations. The aim of this study was to estimate prevalence and clinical characteristics of PCOS in a community setting in Iran.
In a community-based cross-sectional study 401 young women aged 18-33 years selected by cluster sampling proportionate to population size. An interviewer-administered questionnaire was used to screen predisposing factors for PCOS based on menstrual history and clinical manifestations of hyperandrogenism. PCOS be defined presence of least two criteria were considered diagnostic of PCOS, after exclusion of other etiologies.
The overall prevalence of PCOS among young women was 19.0% (CI 95%; 15.0%, 23%). Compared with non-PCOS young women, The adjusted age OR of young women PCOS for problem in abnormal hair growth 5.07 (CI, 2.87-8.99; P The results of this study indicated that counseling and evaluating of the women with irregular cycle and clinical hyperandrogenism using an interviewer-administered questionnaire may be benefit for diagnosis of PCOS.

Preterm labor is a leading cause of fetal and neonatal morbidity and mortality. There are various kinds of drugs used to suppress the preterm labor, but they are not thoroughly effective. The aim of this study was to compare the effectiveness of oral nifedipine with intravenous magnesium sulfate in delaying the preterm labor.

A randomized, clinical trial was conducted in a hospital in Babol, Iran. One hundred twenty singleton pregnant women with preterm labor, 24-37 weeks of gestation, were randomly assigned to receive oral nifedipine or intravenous magnesium sulfate. The main outcome of the study was the inhibition of uterine and the secondary outcome was the side effect related to drugs and neonatal outcome. The data were analyzed with SPSS software, using chi-squared test and independent t test.

According to the results, in 35% of women in the nifedipine group and 23.3% of women in the magnesium sulfate group, the inhibited uterine contraction was less than 48 hours. Also, in 65.0% of women in the nifedipine group and 76.7% of women in the magnesium sulfate group, the inhibited uterine contraction was more than 48 hours. There was no significant difference between the nifedipine and the magnesium sulfate groups in the inhibition of uterine contraction in both less and more than 48 hours. The total side effects of medication were found to be lower in patients receiving oral nifedipine than those who received intravenous magnesium sulfate. (26.6 vs. 45.0) (p= 0.036). There was no significant difference in neonatal outcome between the two groups.

Oral nifedipine should be a suitable alternative to intravenous magnesium sulfate in suppression preterm labor with fewer side effects.

Iatrogenic vas deferens injuries (IVDIs) as inadvertently medical conditions are critical complications of surgical operations in the inguinal region of children and adults. It is well documented that any scant disturbance in vas deferens tunica muscularis or mucosa will endanger fertility and unintentionally manipulations during inguinal hernia repair can cause perforation and/or obstruction of damaged vas deferens resulting in male fertility reduction. It has also been suggested that IVDIs can play crucial roles in formation of anti-sperm autoantibodies and sympathetic vasospasm leading to bilateral testicular deterioration as well as sperm motility and capacitation impairments. In line with that, it seems that non-recognized IVDIs may have destructive effects on spermatogenesis resulting in fertility problems and careful attention to surgical detail should be considered during inguinal region surgical procedures to prevent IVDIs. However, recent progresses in urological microsurgeries and assisted reproductive technologies provide remarkable improvements in fertility outcomes of patients even with serious iatrogenic reduction in fertility.

 

 
 

 

 

 



both mothers and fetuses. The aim of the study was to investigate the effect of dexamethasone on the augmentation and the duration of labor in prolonged latent phase of labor.

The design of the study was a randomized double-blind clinical trial design, which was conducted in delivery center of Rouhani hospital in Babol (Iran). A total 121 nulliparrous women with a singleton pregnancy and cephalic presentation at 40–42 gestational weeks in prolonged latent phase were randomly assigned to receive 2 ml ampoule dexamethasone 4 mg/mL (the intervention group) and 2 ml ampoule of sterile water for injections (the control group), which were both intramuscularly administered. Then, the Then, the augmentation of labor with the use of intravenous oxytocin infusion (Caspian Tamin Company Iran) (2.5 m units/ per minute) began in both groups. The primary outcome was the duration of time between the onset of augmentation and the second stage of labor.

The duration of time between the onset of labor augmentation and the second stage of labor (hours) was 5.6±1.9 in the study group, whereas it was 7.7±1.5 in controls with a significant difference (p ≤ <0.001). In the study group, the duration of time between the onset of labor augmentation and the active phase of labor was lower than that of the control group (p= 0.02). In addition, the duration of the second stage of labor (p <0.001) and the third stage (p <0.001) was lower in the study group compared with that of the control group.

It is imperative that midwives administer dexamethasone to improve the prolonged latent phase in women during the labour.

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