- Volume:11 Issue: 4, 2018
- تاریخ انتشار: 1396/10/28
- تعداد عناوین: 12
Effects of systemic administration of HESA-A on the expression of cyclin D1 and EGFR and E-cadherin in the induced tongue dysplasia in ratsPages 201-207Background. HESA-A has herbal and marine bases, containing minerals and rare elements such as Zr, Cr, Ga, Mn, Mg, Ca, Sr, Cu, Ti, etc. Its mechanism of action includes antioxidant, antiinflammatory and adjustment of the immune system. The aim of this study was to evaluate the effects of HESA-A systemic drug on expression of cyclin D1, EGFR and E-cadherin in induced tongue dysplasia in rats.
Methods. In this experimental study, the effects of the systemic drug HESA-A on the expression of cyclin D1, EGFR, and E-cadherin molecular markers were examined in induced tongue dysplasia in rats.
Results. The incidence rate of cyclin D1 in groups receiving HESA-A was lower than the group that did not receive the drug (77.78% in the 0‒5% range versus 77.78% in the 5‒50% range). In the case of expression of E-cadherin in group D, which did not receive HESA-A, a decrease was observed in the expression of this cell adhesion marker as compared to the other two groups. The incidence of E-cadherin was dependent on HESA-A dose, while with 500 mg/kg it was higher than other groups (>75% in 55.55% versus >75% in 11.11%). Concerning the incidence of EGFR in all the three groups most cases were grade 0.
Conclusion. The results of the present research indicated that considering changes in the expression of cyclin D1 and E-cadherin markers in groups treated with HESA-A, HESA-A® has preventive effects on development of cancer in dysplastic lesions through regulation of expression of these molecules.Keywords: Biomarkers, cadherin, carcinoma, EGFR protein, squamous cell carcinoma, rat
Pages 208-214Background. Squamous cell carcinoma is a prevalent carcinoma of the oral cavity. Recently anti-proliferative effect of probiotics has been considered and assessed against different cancers. The aim of this study was to evaluate the cytotoxicity of Acetobacter syzygii strain supernatant on KB human oral cancer cell line and KDR human epithelial normal cell line.
Methods. The cytotoxicity assessments were performed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as well as through qualitative (4',6-diamidino-2-phenylindole staining) and quantitative (flow cytometry assessments using the BD Biosciences Annexin V-FITC Kit) evaluations of apoptosis.
Results. A. syzygii secretions exhibited significant cytotoxicity against KB cancer cell lines similar to cisplatin (75.7% apoptosis) while the rate of apoptosis in KDR normal cells was only 9.36%. The prophylactic effects of Lactobacillus acidophilus (PTCC 1643), as a reference bacterium, was similar to A. syzygii, indicating beneficial effects of useful bacteria on prevention of oral diseases.
Conclusion. The anticancer bioactivity of A. syzygii strain secretions depends on the induction of apoptosis in KB cancer cells. However, several investigations should be conducted to precisely determine effective compounds to be used as anticancer therapeutics in the future.Keywords: Apoptosis, anticancer, probiotics, squamous cell carcinoma
Antibacterial effect of different concentrations of sodium hypochlorite on Enterococcus faecalis biofilms in root canalsPages 215-221Background. The aim of this study was to evaluate the effectiveness of different concentrations of sodium hypochlorite (NaOCl) solution in reducing bacterial growth in Enterococcus faecalis biofilms in root canals.
Methods. The root canals of maxillary central incisors of 104 subjects underwent chemomechanical debridement. In order to remove the smear layer, 5.25% sodium hypochlorite solution was used for 3 minutes in the root canals. Then, the samples were immersed in 1 mL of 17% EDTA for 3 minutes. Finally, the root canals were irrigated with phosphate-buffered saline (PBS) solution. After removing the smear layer, the samples were sterilized. Then E. faecalis biofilms formed within the root canals at 4-, 6-, and 10-week intervals were evaluated. Each group was divided into 4 subgroups in terms of the antibacterial treatment: group 1: 1% NaOCl solution; group 2: 2.5% NaOCl solution; group 3: 5.25% NaOCl solution; and group 4: PBS solution. After preparation of root canal filings, the counts of live bacteria were calculated through the classic method of counting, i.e. colony forming units (CFU), followed by the analysis of data.
Results. In groups 2 and 3, there was no bacterial growth due to complete removal of E. faecalis biofilms (PConclusion. The bacterial cells in mature and old biofilms have higher resistance to 1% NaOCl solution compared to the young biofilms. However, the 2.5% and 5.25% NaOCl solutions caused complete inhibition of the growth of E. faecalis biofilm in all the stages of development.Keywords: Antibacterial, biofilm, Enterococcus faecalis, sodium hypochlorite
Effect of preheat repetition on color stability of methacrylate- and silorane-based composite resinsPages 222-228Background. The aim of this study was to investigate the effect of preheating methacrylate- and silorane-based composite resins on their color stability up to 40 times at 55‒60°C.
Methods. Seventy-six methacrylate and silorane-based composite resin samples, with a diameter of 10 mm and a height of 2 mm, were divided into 4 groups (n=19). After the samples were prepared, their color parameters were determined using a reflective spectrophotometer. The composite resin samples were separately stored in a solution of tea for 40 consecutive days. Then the samples underwent a color determination procedure again using a spectrophotometer and color changes were recorded. Finally two-way ANOVA was used to study the effect of composite temperature on its staining (PResults. Both composite resin type (P=0.014) and preheating (PConclusion. Repeated preheating of methacrylate- and silorane-based composite resin samples, up to 55‒60°C for 40 rounds, resulted in more color changes compared with unheated composite resin samples. After storage in a solution of tea the color change rate in the composite resin samples of silorane-based was higher than the Z250 composite resin samples.Keywords: Composite resin, preheating, microleakage, color stability
Clinical and histological evaluation of increase in the residual ridge width using mineralized corticocancellous block allografts: A pilot studyPages 229-235Background. Lateral ridge augmentation is conventionally accomplished by means of autogenous bone grafts. However, due to its complications, the application of autogenous bone graft substitutes, e.g. mineralized corticocancellous allograft, is recommended.
Methods. In the present study, twelve patients were included, with insufficient alveolar ridge widths in the designated sites for dental implant placement. During the primary surgery, mineralized corticocancellous block allografts were fixed in deficient sites with titanium screws and resorbable collagen membranes were used to cover the blocks. After a period of six months, a flap was raised and variations in ridge width values was measured. Finally, a micro-biopsy was obtained from the sites for histologic investigation prior to preparing them for subsequent implant placement.
Results. All the applied blocks were incorporated into the underlying bone except for one. A statistically significant difference was seen between the average ridge widths before placing the allografts compared with that of implant placement stage (2.62±1.02 mm vs. 7.75±1.63 mm, respectively). Vital bone tissue was detected in all the histological specimens obtained from the interface of blocks and the underlying bone.
Conclusion. The results suggest that mineralized corticocancellous block allografts might be used as scaffolds for bone growth and ridge width augmentation.Keywords: Allografts, alveolar ridge augmentation, dental implants
Effects of adjective use of melatonin and vitamin C in the treatment of chronic periodontitis: A randomized clinical trialPages 236-240Background. Melatonin can eliminate free radicals and this function can be intensified byvitamin C. Melatonin and vitamin C can have synergic antioxidant effects. The aim of thisstudy was to investigate the effects of adjunctive use of melatonin and vitamin C in the non-surgical treatment of chronic periodontitis.
Methods. Sixty subjects with chronic periodontitis were included in this study and randomly allocated to three groups: group 1) 20 patients received non-surgical periodontal treatment; group 2) 20 patients received non-surgical periodontal treatment with adjunctive use of melatonin; and group3) 20 patients received non-surgical periodontal treatment with combination use of melatonin vitamin C. Clinical parameters (PD, CAL,GI) were recorded at baseline and at 3-month and 6-month intervalsafter treatment. Data were analyzed with paired t-test, one-way ANOVA andrepeated-measures ANOVA. A significant difference was assumed at PResults. Non-surgical periodontal therapy improved PD and CAL 3 and 6 months treatment compared to baseline (P0.05). Therefore adjunctive dose of vitamin C offered an additional effect at this interval.
Conclusion. Combination therapy with melatonin and vitamin C can improve the results of non-surgical periodontal therapy.Keywords: Dental scaling, melatonin, periodontitis, vitamin C
Bending resistance and cyclic fatigue life of Reciproc Blue, WaveOne Gold, and Genius files in a double (S-shaped) curved canalPages 241-246Background. This study compared the cyclic fatigue resistance, bending resistance and cross-sectional areas of Reciproc Blue (RPC Blue), WaveOne Gold (WOG), and Genius File (GF) NiTi rotary systems.
Methods. Forty RPC Blue R25 (25/.08), 40 WOG Primary (25/.07) and 40 GF (25/.04) files were used in the present study. Flexibility of the files was determined by 45° bending test. The instruments were also subjected to cyclic fatigue resistance, calculating the number of cycles to fracture (NCF) in an S-shaped artificial stainless steel canal. Also the cross-sectional areas of the files were measured at D5 level. The length of the fractured file tips was measured. The fracture surface of all the fragments was examined with a scanning electron microscope. Data was statistically analyzed using one-way ANOVA and post hoc Tukey tests.
Results. In both the apical and coronal curvatures, the NCF of the GF was significantly higher than that of the RPC Blue and WOG files. There was no significant difference between the GF, WOG and Reciproc Blue files with respect to the lengths of the fractured file fragments in either the apical or coronal curvature. The bending resistance of the GF was signif-icantly higher than that of the RPC Blue and WOG files. The RPC Blue had the largest cross-sectional area, and the GF had the smallest cross-sectional area.
Conclusion. Within the limitations of the present study, the GF NiTi system exhibited the highest cyclic and bending re-sistance among the experimental groups.Keywords: Cyclic Fatigue, Cross-section Area, Double Curvature, Genius File, Reciproc Blue, WaveOne Gold
Pages 247-252Background. Unstimulated salivary flow rate can be influenced by different factors. This study was undertaken to evaluate the effect of stress, anxiety and depression on unstimulated salivary flow rate in adults.
Methods. A total of 247 adult subjects, randomly selected from patients referring to Zanjan Dental School, were included in this investigation. The study procedures consisted of collecting salivary samples (in 5 minutes), completing a form for feeling of xerostomia and completing Depression Anxiety Stress Scale (DASS) Questionnaire to assess the severity of stress, anxiety and depression. Based on the results, the patients were categorized in four groups: Low salivary flow rate plus xerostomia (group 1, n=60), normal salivary flow rate plus xerostomia (group 2, n=59), low salivary flow rate without xerostomia (group 3, n=60) and normal salivary flow rate without xerostomia (control group, n=68).
Results. The frequencies of subjects with severe and major depression in groups 1, 2 and 3 were 31.4%, 11.7% and 8.5%, respectively, with 4.4% in the control group. The frequencies of subjects with severe stress in groups 1, 2 and 3 were 21.7%, 3.3% and 11.9%, respectively, with 1.5% in the control group. The frequencies of patients with severe anxiety in groups 1, 2 and 3 were 50%, 30% and 61.1%, respectively, with 4.4% in the control group. Stress, anxiety and depression exhibited a statistically significant relationship with unstimulated salivary flow rate and xerostomia (PConclusion. Stress, anxiety and depression can influence unstimulated salivary flow rate and lead to xerostomia.Keywords: Anxiety, depression, saliva, flow rate, stress disorder, xerostomia
Pages 253-256Infants with cleft palate (CP) suffer from several difficulties, one of them is feeding, which prevent infant from maintaining adequate nutrition. Usage of feeding plate, special bottles and nipples has been described to overcome this problem. This article describes steps of constructing a single visit feeding plate for a CP infant. The main objective was to provide an infant with a properly functioning feeding prosthesis and to reduce parents anxiety originated from multiple dental visits. We can conclude that our feeding plate was successfully achieving the planned objectives.Keywords: Feeding, Cleft palate, Obturators, Infant
Effect of local injection of Zolena, zoledronic acid made in Iran, on orthodontic tooth movement and root and bone resorption in ratsPages 257-264Background. Anchorage control is an essential part of orthodontic treatment planning, especially in adult patients who demand a more convenient treatment. Zoledronic acid (ZA) is an effective choice to address this problem. It is the most potent member of the bisphosphonates family that has an inhibitory effect on bone resorption by suppressing osteoclast function. Therefore, ZA might be a good option for orthodontic anchorage control. The current study evaluated the effect of local administration of Zolena (ZA made in Iran) on orthodontic tooth movement (OTM) and root and bone resorption.
Methods. The experimental group consisted of 30 rats in 3 subgroups (n=10). Anesthesia was induced, and one closed NiTi coil spring was installed between the first molar and central incisor unilaterally, except for the negative control group. The positive control group received vestibular injection of 0.01 mL of saline next to the maxillary first molar, and 0.01 mL of the solution was injected at the same site in the ZA group. After 21 days, the rats were sacrificed and the distance between the first and second molars was measured with a leaf gauge. Histological analysis was conducted by a blind pathologist for the number of Howships lacunae, blood vessels, osteoclast-like cells and root resorption lacunae. Data were analyzed with ANOVA, Tukey test and t-test.
Results. There were no significant differences in OTM between the force-applied groups. ZA significantly inhibited bone/root resorption and angiogenesis compared to the positive control group.
Conclusion. Zolena did not decrease OTM but significantly inhibited bone and root resorption. Zolena might be less potent than its foreign counterparts.Keywords: Bone resorption, rat, root resorption, tooth movement, zolendronic acid
Pages 265-274The field of health has always emphasised on the use of natural products for curing diseases. There are varieties of natural products (such as silk, herbal tea, chitosan) used today in the biomedical application in treating a large array of systemic diseases. The natural product "Propolis" is a non-toxic resinous material having beneficial properties such as antimicrobial, anticancer, antifungal, antiviral and anti-inflammatory; hence gain the attention of researchers for its potential for bio-dental applications. The study aims to explore the properties and chemistry of propolis concerning biomedical and dental applications. In addition, status and scope of propolis for current and potential future in bio-dental applications have been discussed. This review gives an insight to the reader about the possible use of propolis in modern-day dentistry.Keywords: Antimicrobial, dentistry, natural biomaterials, oral health care, propolis, restorations