فهرست مطالب

Organ Transplantation Medicine - Volume:9 Issue: 1, 2018
  • Volume:9 Issue: 1, 2018
  • تاریخ انتشار: 1396/11/20
  • تعداد عناوین: 8
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  • B. Palkoci, M. Vojtko, J. Fialov, Aacute, D. Osinov, Aacute, M. LajČ, Iakov, Aacute Page 1
    Background
    Collection of kidneys from extended criteria donors (ECD) with diagnosed brain-death forms a part of the collection program that increases the number of transplantations.
    Objective
    To compare the results of ECD with those of standard criteria donors (SCD).
    Methods
    In a retrospective analysis in a group of 156 kidney donors, we identified ECD donors. We detected the basic parameters of the donors before kidney collection, and then evaluated the function of the graft, the survival of the graft, and the survival of the patients after 1, 3, and 5 years of transplantation. The results were then compared with the function of the graft from those of SCD donors.
    Results
    The ECD donors were significantly (p
    Conclusion
    The long-term function of the graft and survival of patients and grafts in recipients of kidneys from ECD donors are comparable to SCD donors. Exploitation of the given organs for transplantation is important due to the constantly increasing demand versus limited offer of organs.
    Keywords: Organ donor, Transplant donor, Donor, tissue, Cadaver, Brain Death, Donor after brain death, Extended criteria donors, Kidney transplantation
  • T. BenkÖ_M. Gottmann_S. Radunz_A. Bienholz_F. H Saner_J. W Treckmann_A. Paul_D. P Hoyer Page 10
    Background
    Antiplatelet therapy is common in patients on the waiting list for kidney transplantation.
    Objective
    To evaluate the incidence of post-operative bleeding in patients with antiplatelet therapy undergoing kidney transplantation and analyze the impact on the outcome.
    Methods
    We studied all patients with concomitant antiplatelet therapy undergoing kidney transplantation in our center from January 2007 to June 2012. Data were collected by chart review. Univariate and multivariate logistic regression and Cox proportional hazard model were used to identify risk factors for the long-term outcome.
    Results
    Of 744 kidney transplant recipients during the study period, 161 received oral antiplatelet therapy and were included in the study. One-third of the patients demonstrated signs of bleeding, half of which requiring surgical treatment. Coronary artery disease, deceased donor kidney transplantation, and dual antiplatelet medication were independent risk factors for post-operative bleeding. One-year allograft survival was significantly better in the non-bleeding group (91.4% vs 75.9%, p=0.023). Multivariable analysis found that post-operative bleeding, recipient age, and biopsy-proven rejection were independent risk factors for graft survival. Recipient age and biopsy-proven rejection were also identified as independent risk factors for patient survival.
    Conclusion
    This analysis indicated a high risk for post-operative bleeding in renal transplant patients under antiplatelet therapy. The associated negative effect on allograft survival underscored the need to reduce any risk factors for post-operative bleeding.
    Keywords: Platelet aggregation inhibitors, Kidney transplantation, Graft survival, Postoperative period, Postoperative complications
  • A. J Perez_I. N Haskins_A. S Prabhu_D. M Krpata_C. Tu_S. Rosenblatt_K. Hashimoto_T. Diago_B. Eghtesad_Mij Rosen Page 20
    Background
    Umbilical hernias are common in patients with end-stage liver disease undergoing liver transplantation. Management of those persisting at the time of liver transplantation is important to define.
    Objective
    To evaluate the long-term results of patients undergoing simultaneous primary umbilical hernia repair (UHR) at the time of liver transplantation at a single institution.
    Methods
    Retrospective chart review was performed on patients undergoing simultaneous UHR and liver transplantation from 2010 through 2016. 30-day morbidity and mortality outcomes and long-term hernia recurrence were investigated.
    Results
    59 patients had primary UHR at the time of liver transplantation. All hernias were reducible with no overlying skin breakdown or leakage of ascites. 30-day morbidity and mortality included 5 (8%) superficial surgical site infections, 1 (2%) deep surgical site infection, and 7 (12%) organ space infections. Unrelated to the UHR, 10 (17%) patients had an unplanned return to the operating room, 16 (27%) were readmitted within 30 days of their index operation, and 1 (2%) patient died. With a mean follow-up of 21.8 months, 7 (18%) patients experienced an umbilical hernia recurrence.
    Conclusion
    Despite the high perioperative morbidity associated with the transplant procedure, concurrent primary UHR resulted in an acceptable long-term recurrence rate with minimal associated morbidity.
    Keywords: Cirrhosis, Clinical decision, making, Liver disease, Tissue injury, repair, Surgical technique, Umbilical hernia, Liver transplantation
  • Z. Hashemizadeh, Sa Malek-Hosseini, P. Badiee Page 27
    Background
    Patients with hepatic diseases are treated with numerous drugs metabolized by cytochrome P450.
    Objective
    To evaluate the frequencies of CYP2C19 variant alleles (*2, *3, and *17), genotypes, and phenotypes, and the relationship between the frequency of these alleles and the underlying hepatic diseases among patients with advanced liver diseases who were candidates for liver transplantation.
    Methods
    The Study was conducted on 120 patients suffering from various hepatic disorders, candidates for liver transplantation, and 52 healthy volunteers. DNA was extracted from blood samples and analyzed by TaqMan SNP genotyping assay. The CYP2C19 genotypes were classified into poor, extensive, intermediate, and ultra-rapid metabolizer phenotypes.
    Results
    Viral hepatitis was the most common cause of liver disease among studied patients. The frequencies of CYP2C19 alleles *1, *17, and *2 were 66.7% (160/240), 20.8% (50/240) and 12.5% (30/240), respectively. Allele CYP2C19*3 was not found in the studied population. The most prevalent genotypes were CYP2C19 *1/*1 (47.5%) and *1/*17 (24.2%). The predicted CYP2C19 phenotypes were extensive metabolizer (47.5%), heterozygote extensive metabolizer (45.9%), ultra-rapid metabolizer (5%), and poor metabolizer (1.6%). There was no significant difference between the frequencies of CYP2C19 genotypes between healthy people and patients. The distribution of CYP2C19 genotype frequencies was not significantly associated with the underlying disease conditions (p=0.472).
    Conclusion
    The distribution of CYP2C19 genotype frequencies in Iranian healthy people and patients with various hepatic diseases was not significantly different. This may allow the physicians to predict a tailoring dose regimens based on the individual’s metabolic capacity, decrease the risk of harmful side effects of the drugs, and optimize the treatment.
    Keywords: Cytochrome P450, CYP2C19, Hepatic disorders, Liver transplantation
  • M. Nasiri, B. Geramizadeh, Sh Nabavizadeh, Sa Malek-Hosseini, Mh Karimi, I. Saadat Page 34
    Background
    Interferon regulatory factors (IRFs) can play a critical role in the regulation of many facets of innate and adaptive immune responses through transcriptional activation of type I interferons, other proinflammatory cytokines, and chemokines. However, their roles in transplantation immunity still remain to be elucidated.
    Objective
    To evaluate the time course of mRNA expression of all 9 members of IRFs family of transcription factors during liver allograft acute rejection.
    Methods
    Blood samples of 19 patients with autoimmune hepatitis receiving liver transplants were collected on days 1, 3, 5, and 7 post-transplantation. The patients were followed for 6 months after transplantation and divided into two groups of acute rejection (AR) (n=4) and non-acute rejection (non-AR) (n=15).
    Results
    All of the studied transcription factors were down-regulated in AR-group on days 3, 5, and 7 post-transplantation compared to non-AR group. The mean±SEM IRF5 on day 7 post-transplantation was significantly (p=0.005) lower in AR-group than in non-AR group (0.7±0.21 vs. 1.91±0.27, respectively); expression of other IRFs family members was not significantly different between the two groups on days 3, 5, and 7 post-transplantation.
    Conclusion
    IRF5 may have an important role during the acute rejection of liver transplants.
    Keywords: Interferon regulatory factor, Liver transplantation, Graft rejection, Hepatitis, autoimmune, Toll like receptor, RNA, Messenger, transcription factors
  • Sm Dehghani, I. Shahramian, M. Afshari, M. Bahmanyar, M. Ataollahi, A. Sargazi Page 41
    Background
    Acute cellular rejection (ACR), a reversible process, can affect the graft survival.
    Objective
    To evaluate the relation between ACR and clinical factors in recipients of allograft liver transplantation.
    Methods
    47 recipients of liver were consecutively enrolled in a retrospective study. Their information were retrieved from their medical records and analyzed.
    Results
    Of the 47 recipients, 38 (81%) experienced acute rejection during 24 months of the transplantation. None of the studied factors for occurring transplant rejection, i.e., blood groups, sex, age, familial history of disease, receiving drugs and blood products, type of donor, Child score, and Child class, was not found to be significant.
    Conclusion
    During a limited follow-up period, we did not find any association between ACR and suspected risk factors.
    Keywords: Acute cellular rejection, Liver transplantation, Pediatric, Graft survival, Transplant recipients, Graft rejection
  • S. Gooran, A. Javid, G. Pourmand Page 46
    One of the most catastrophic complications of kidney transplantation is non-traumatic delayed bleeding caused by arterial dissection and pseudoaneurysm, endangering the survival of the graft and the patient. Herein, we discuss the management of this condition in 3 cases.
    The patients included 2 men, 30 and 47 years old, and a 33-year-old woman, who developed a massive hemorrhage in the second week after kidney transplant. All our patients were diabetic for more than 5 years. Massive hemorrhage occurred in the second week without any trauma or precipitating factor. A combination of antibiotic therapy, surgery and interventional procedures was required and all three transplanted kidneys inevitably had to be removed. Although there were trivial signs of infection, considerable pus and infectious and necrotic tissue were drained during graft nephrectomy. A high index of suspicion is necessary for the timely diagnosis of arterial dissection and aneurysm. Aggressive treatment with arterial drug-eluting stents and surgical drainage are necessary in order to prevent potentially fatal complications.
    Keywords: Renal transplantation, Aneurysm, Aneurysm, false, Post, operative hemorrhage
  • V. Ince, C. Kayaalp, E. Otan, F. Ozdemir, A. Dirican, Hi Toprak, C. Aydin, C. Ara, S. Yilmaz Page 50
    Emergency liver transplantation (LT) for acute liver failure (ALF) is a life-saving treatment. Occurrence of this situation in the same patient twice is very rare. Herein, we describe a patient who underwent two emergency LTs for ALF, both from living donors. When she was 26 years old, she underwent a right lobe living donor LT (LDLT) from her sister for ALF due to use of herbal weight loss medications. The next 3 years were uneventful but another ALF developed during a terminal stage pregnancy (37th week). Despite medical treatment, her liver functions worsened, and the baby was delivered by caesarean section. The second time, her brother was the donor and she recovered after the emergency right lobe re-LDLT. Both patient and baby were well at the 2-month follow-up. As far as we know, there is no reported similar case, and we concluded that LDLT is a paramount treatment option for both primary and secondary ALFs.
    Keywords: Liver failure, acute, Living donors, Transplantation