فهرست مطالب

  • Volume:8 Issue: 4, 2018
  • تاریخ انتشار: 1397/07/25
  • تعداد عناوین: 7
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  • Masumeh Sanaei *, Fraidoon Kavoosi * Pages 193-201
    Background
    Hepatocellular carcinoma (HCC), primary liver cancer, is a major health problem and the third most common cause of cancer-related deaths worldwide. Epigenetic modulations are essential for the maintenance of gene expression patterns in mammals. Disruption of these processes can lead to silenced gene and malignant cellular transformation. The current study was designed to compare the effect of curcumin with trichostatin A (TSA) on DNA methyltransferase 1 (DNMT1) gene expression, cell growth inhibition, and apoptosis induction in HCC Hepa 1-6 cell line.
    Materials and Methods
    Hepatocellular carcinoma Hepa 1-6 cell line was purchased from the National Cell Bank of Iran-Pasteur Institute, treated with curcumin (1, 5, 10, 25 and 50 μM) and TSA (0.5, 1, 2.5, 5 and 10 μM), and the MTT assay was performed. Then, flow cytometry assay and Real-Time RT-PCR analysis were performed with curcumin and TSA treatments. Statistical comparisons between groups were performed using ANOVA (one‑way ANOVA) and Turkey test. A significant difference was considered as P < 0.05.
    Results
    Both treatments showed significant inhibitory and apoptotic effects, besides reducing the expression of DNMT1. The relative expression of DNMT1 gene in the curcumin-treated groups were 0.7 to 0.3 (P <0.001) and in the TSA treated groups were 0.5 to 0.19 (P <0.001).
    Conclusion
    The curcumin and trichostatin A (TSA) can inhibit cell viability and induce apoptosis somehow through epigenetic modification. The curcumin indicated a more significant apoptotic effect than TSA.
    Keywords: Apoptosis, Curcumin, DNMT1, Trichostatin
  • Rahmatolah Khanmohammadi , Laya Sadat Khorsandi , Maghsud Peeri, Mohammad Ali Azerbaijani * Pages 202-212
    Background
    Doxorubicin (DOX) is an anthracyclines antibiotic and is one of the most effective antitumor drugs. However, using this drug is associated with poisoning of healthy tissues. The purpose of this study was to investigate the changes in hematological parameters of male rats exposed to doxorubicin after eight weeks of high-intensity interval training and crocin consumption.
    Materials and Methods
    In the present experimental research, 50 male Wistar rats (weight 200-220 gr) were exposed to intraperitoneal injections of doxorubicin (2mg/kg, seven times for 7weeks) and randomly placed into five groups, including healthy control (saline) group, doxorubin group, doxorubicin with Crocin group, doxorubicin with training group, and doxorubicin with crocin a long with training group. The training groups completed the course with two intense interval training during the first week and completed 8 intense interval training in the last weeks. Forty eight hours after the last training session, blood samples were taken directly from the heart of rats to measure the desired indices of red blood cell (RBC), Hematocrit(HCT), Hemoglobin(HB), platelet(PLT), white blood cell(WBC), and percentage of lymphocytes(%LYM), and neutrophils (%NEU). Independent sample t-test as well as two-way ANOVA were used for data analysis. P value <0.01 was set as significant level.
    Results
    The results showed that DOX significantly reduced all of the blood parameters in rats in comparison to the control group (p<0.01). Eight-week high intensity interval training significantly increased blood parameters (p<0.01). In Crocin- treated rats, the blood parameters were significantly increased compared with the DOX-intoxicated animals (p<0.01). In Crocin and high intensity interval training group, the blood parameters were significantly increased compared with the DOX-intoxicated animals (p<0.01).
    Conclusion
    It seems that high-intensity interval training, Crocin, as well as combination of these two interventions can prevent severe reduction of hematological parameters in rats treated with doxorubicin.
    Keywords: Crocin, Doxorubicin, Hematological Parameters, High intensity interval training
  • Mahbubeh Hosseini Dr, Durdi Qujeq Dr, Mohammad Ranaee Dr, Karimollah Hajian Tilaki Dr, Ahmad Tamaddoni Dr * Pages 213-220
    Background
    This study aimed to compare the effect of the aqueous extracts of Anethum graveolens, Urtica dioica, and Silybum marianum with deferoxamine (DFO)on iron-overloaded rats.
    Materials and Methods
    In this case control study, fresh leaves of Anethum graveolens and Urtica dioica as well as seeds of Silybum marianum were washed with water and rinsed with deionized water to remove possible pesticide and preservative residue. Forty eight male rats were randomly divided into six groups: negative control, positive control (iron overload), Anethum graveolens group, Urtica dioica group, Silybum marianum group, , and DFO group. Iron dextran was injected intraperitoneally (i.p.) at 50 mg/kg body weight for 12 weeks (once a week) to establish the iron overload condition. While normal rats received normal saline, rats in the treated groups orally received three plant extracts and DFO (subcutaneously) weekly for 8 weeks (once a week). At the end of the experiment, changes in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were measured using AST and ALT kits from Zist Chem Diagnostics Co. (Tehran, Iran) and histological changes were observed by hematoxylin and eosin staining of the liver.
    Results
    Iron overloaded rats showed a slightly increase in the serum ALT and AST activity compared to control group (4.97±0.12, 60.79±1.16 vs. 3.92±0.10, 50.24±0.82U/L, respectively) (p value< 0.05). However, relative to iron overloaded rats, treatment with Anethum graveolens, Urtica dioica, and Silybum marianum extracts and DFO significantly decreased the activity of ALT and AST (AlT: activity (4.37 ±0.08, 4.58±0.06, 4.74 ±0.09 and  3.96 ±.015 U/L , respectively );( AST: 53.68±1.24, 55.78±1.03, 57.87±1.15 and  51.65 ±0.66 U/L, respectively) (p value< 0.05). Treatment of iron overloaded rats with Anethum graveolens, Urtica dioica, and Silybum marianum also significantly improved the liver dysfunction.
    Conclusion
    Anethum graveolens, Urtica dioica, and Silybum marianum may be potential medicinal herbs which can reduce liver damage caused by iron overload.
    Keywords: Anethum graveolens, Deferoxamine, Iron-overloaded, Silybum marianum, Urtica dioica
  • Mahbod Kaveh Dr, Shiva Akhbari Dr, Kamyar Kamrani Dr * Pages 221-227
    Background
    Neonatal sepsis is a generalized bacterial infection which occurs in the first month of life. Intraventricular hemorrhage is the most common intracerebral disorder which occurs in premature neonates. Mean Platelet Volume (MPV) is considered as a marker of intraventricular hemorrhage (IVH) in some studies. The aim of this study was to evaluate the impact of MPV on IVH and sepsis in neonates.
    Materials and methods
    In this retrospective case-control study, 20 premature neonates with sepsis and 20 with sepsis and intraventricular hemorrhage were considered as case groups and 20 premature neonates without sepsis and IVH were regarded as control group. Demographic data as well as patients' data on IVH presence, IVH grading, mortality, platelet account, and MPVon the first and third days after birth were recorded using their medical files. After data collection, analysis was performed using SPSS (version 21) and running descriptive and analytical methods (T test, ANOVA and Chi square test).
    Results
    In this study, 10 newborns (50%) in the sepsis group, 13 newborns (65%) in the IVH – sepsis group, and 10 newborns (50%) in the control group were male (P = 0.523). Mean ± standard deviation of gestational age and weight at birth were significantly lower in the IVH and sepsis group in comparison with the other two groups (P-value < 0.001). Considering platelet count on first day, no significant difference was observed among three groups; however, it was lower in the control group than the sepsis group as well as the IVH and sepsis group (P=0.004). Gender, birth weight, gestational age, onset of sepsis, and presence of respiratory distress syndrome (RDS) could not make significant changes in MPV three groups.
    Conclusion
    The results showed that MPV on the first day was significantly higher in patients with sepsis or with sepsis and IVH.
    Keywords: Hemorrhage, Mean Platelet Volume, Newborn, Sepsis
  • Ahmad Jzizamei Khosro Dr, Aziz Hasanzadeh Dr, Maryam Banihosseinian Dr, Amirataollah Hiradfar Dr * Pages 228-236
    Background
    The purpose of this study was to investigate efficacy of high dose vitamin D in improving left ventricular ejection fraction (LVEF) in thalassemia patients with heart failure and vitamin D deficiency.
    Materials and Methods
    This clinical trial study was conducted on 16 chronically transfused thalassemia patients and ventricular dysfunction with vitamin D deficiency between December and Jun 2018 in Thalassemia clinic, Tabriz Children Hospital. Mean age of the patients was 11.15 ± 3.61 years ranged from 8 to 18 years old. A serum 25-hydroxy vitamin D3 (25-OHD3) level less than 30ng/dl was considered vitamin D deficiency in this study. LVEF less than 55% was indicated as poor pump function. The patients received 50,000 IU of vitamin D3 weekly for 8 weeks. Data on LVEF and serum 25-OHD3 were compared before and after completing the treatment. Moreover, adverse effects were recorded during the study.
    Results
    Means of serum 25-OHD3 levels, before and after the study, were 13.10±5.91ng/ml and 51.03±4.31ng/ml, respectively (p=0.01). Means of LVEF were13.10±5.91% and 50.27±11.93% before and after the study, respectively (p=0.03). Means of serum ferritin levels were 3913±2229 ng/ml (ranged from 1246 to11000ng/ml). Mean of cardiac magnetic resonance imaging (MRI) T2* of the patients was 11.51±5.34ms. Serum parathyroid hormone (PTH) levels of the patients decreased from the beginning of the study to the end of the eighth week (94.28 ± 18.35 vs 43.66 ± 17.31ng/ml) (p=0.03). There was a positive correlation between mean of serum 25-OHD3 level and cardiac MRI T2* parameter at the beginning of the study (r=0.001). There was a positive correlation between in the increase of mean serum 25-OHD3 and LVEF percent at the end of study (r=0.001).
    Conclusion
    Results showed that vitamin D3 was effective and safe in improving LVEF and cardiac dysfunction in transfusion-dependent thalassemia patients with vitamin D deficiency.
    Keywords: Cardiac dysfunction, Thalassemia, Ventricular Dysfunction, Vitamin D
  • Mohammad Javad Forouzani Moghaddam, Parastoo Nabian, Arefeh Gholami, Neda Dehghanbaghi, Mahdieh Azizipanah, Kimia Jokar, Fatemeh Eslami, Zahra Kargarian, Motahhareh Tamehri, Nazanin Zare, Safiehsadat Heydari, Marzieh Esmaeili, Karbasi Najafabadi, Maryam Boyerhasan, Hadi Zare Zardini * Pages 237-246

    Neuroblastoma is considered as the most common solid tumor in children and it is a special types of nervious cells cancer. Neuroblastoma has high potency for metastasis to other organs such as neck, chest, abdomen, or spine. In this narrative review, we assessed prevalence, diagnosis, related factors, and treatment of neuroblastoma based on published articles from 2007 to 2017. All published articles in mentioned interval were evaluated and all required data were collected. The collected data were categorized based on determined outlines. According to our findings, neuroblastoma allocated about 10 percent of pediatric cancer to itself. Mortality rate of this cancer is 15% to20% (annualy 15 per million children aged< 9 years). The incidence of this tumor is higher at the first year of life than other years. The highest incidence is observed in children with age range of 0-5 years. This tumor has low prevalence between people aged > 18 years. Important symptoms of neuroblastoma are: fatigue, loss of appetite, fever, bone pain, blemishes of the skin, a lump in the abdomen, neck, or chest, or a painless bluish lump under the skin, weakness, and slackness. The genes involved in this disease include ALK, BARD1, ERBB2, KIF1B, LMO1, MYCN, PHOX2B, 17q gain, loss of 9p, and 3p, loss of 1p 11q. Surgery, chemotherapy (cyclophosphamide, cisplatin, vincristine, doxorubicin, uteroside, and topotecan), radiotherapy, bone marrow transplantation, and transplantation of peripheral blood stem cells are different type of treatment methods for neuroblastoma. The findings of this review also showed that the use of drug delivery system such as lipidic nanostructures, magnetic nanostructures, and other related devices can improve the treatment of neuroblastoma and reduce the side effects induced by different treatments.

    Keywords: Neuroblastoma, Genetic Factors, Treatment, Chemotherapy
  • Mozhgan Hashemieh Dr * Pages 247-251
    Background
    Hepatic tumors are rare in children comprising only 1% - 4 % of all childhood solid tumors. Primary sarcomas of the liver are extremely rare and represent approximately 0.2 % of all liver tumors. Undifferentiated embryonal sarcoma of liver is an aggressive tumor with an unfavorable prognosis. Here we reported a case of undifferentiated embryonal sarcoma of liver in a 9-year-old boy who was misdiagnosed as hydatid cyst of liver. Case Report: An 8-year-old male patient was referred to our hospital following unsuccessful surgery for presumed hydatid disease of the right lobe of liver. Computed tomography (CT) and ultrasonography showed a large hepatic mass. Following the surgery, our patient received adjuvant chemotherapy. At the time of writing, it has been one year after the termination of adjuvant chemotherapy. Follow up imaging studies, including ultrasonography, CT scan, and MRI show no evidence of recurrence.
    Conclusion
    Here we reported a case of undifferentiated embryonal sarcoma of liver in a 9-year-old boy mistaken for hydatid cyst of liver. Based on our observation, undifferentiated embryonal sarcoma of liver should be considered as a differential diagnosis among patients suspected of hydatid cyst of liver.
    Keywords: Childhood Liver Tumors, Hydatid Cyst, Undifferentiated Embryonal Sarcoma of Liver