مجله دانشکده علوم پزشکی نیشابور
پیاپی 21 (زمستان 1397)

Breast cancer originates from abnormal growth of normal cells in the chest. Like any other types of cancers, breast cancer starts with a single cell. The main aim of this study was to investigate the anti-proliferative effects of Jujube extracts on growth and proliferation of Sk-Br-3 tumor cell line and assessment of BAX expression. After preparing Jujube hydro-alcoholic extract, Sk-Br-3 cell line was treated with Jujube extract in different groups for 24, 48 and 72 hours. Subsequently, it was measured by MTT assay and the rate of apoptosis was recorded. Finally, RNA was extracted from control and treated group by 2.1 (higher than 1.5 mg/ml), 1.5 (between 0.5-1.5 mg/ml) and 0.7 mg/ml (lower than 0.5 mg/ml), transformed into cDNA and the level of BAX gene expression was measured according to the GAPDH gene as a housekeeping gene by Real-Time PCR system. According to MTT results, 2.1, 1.5 and 0.7 mg/ml concentrations were selected as an IC50 for 24, 48 and 72 hours, which was time-dependent and has increased rate of apoptosis and BAX gene expression. In this study, Jujube hydro-alcoholic extract was used for the first time on Sk-Br-3 cell line ever. According to the results, Jujube hydro-alcoholic extract could be suggested as the Sk-Br-3 cell line treatment protocol.

Lack of nutritional knowledge is one of the most important reasons for nutritional problems and consequently im proper practice, which can lead to several complications. The aim of this study was to assess the knowledge, attitude, and practice of urban and rural households toward principles of nutrition in Neyshabour. This cross-sectional study investigates the knowledge, attitude, and practice of urban and rural households towards principles of nutrition in Neyshabour. The sampling method at households' level was single stage cluster sampling with equal size clusters. The incumbent data was collected by a structured questionnaire and through the interview with the eligible subject in each household. A total of 385 households were selected as overall sample size. Around 70% of households were aware of food groups. About 40-70% had correct knowledge about the roles of food groups. Most of the households had accurate knowledge about the reason for food eating. Knowledge, attitude, and practice levels of households toward principles of nutrition weremoderate and good. The results of this study can be used for proper intervention for improving health society.

The best way to prevent osteoporosis, at old age is to prevent bone loss and at young age is trying to keep bones healthy, therefore The aim of this study was to determine the effects of compare effects of aerobic and resistance exercise training protocols with different intensities on microRNA-133a (mir-133a), Runx2 and Peroxisome proliferator-activated receptors-gamma (PPARγ) in femor bone marrow Wistar elderly rats.. This experimental study was done on 40 Wistar male rats . They were divided into 5 equal groups (n=8) incloud moderate or high intensity resistance training, moderate or high intensity endurance training and control. The two training groups completed 8 weeks of training program, 5 days a week according to protocol. After completing training, expression of mir-133a, Runx2 and PPARγ were measured RT-PCR. The statistical analysis was performed using Kruskal-Wallis test with significance level of P<0.05. There was no significance in expression of mir-133a، Runx2 and PPARγ in 5 groups. Investigating cellular changes in bone after such exercises requires longer periods of exercise that should be investigated in future research..

  Celiac disease has been known to be one of the health concerns with an increasing trend worldwide. This autoimmune disease is due to a reaction to gluten resulting in destroyed intestinal villi. Recent studies have shown that Lnc13 affects the pathogenicity of the disease. However, recent studies showed that this group of genes can affect the inflammation due to the disease. We aimed to evaluate the expression of Lnc13 in Iranian celiac patients. In this case-control study, a total of 50 celiac patients and 20 healthy controls blood samples were collected during the 1396-1395 years that referred to the Research Institute for Gastroenterology and Liver Diseases, ShahidBeheshti University of Medical Sciences, Tehran, Iran. To evaluate the relative expression of LncRNA13, the qRT-PCR method was applied. Significantly correlation between Lnc13 gene relative expression in patients with celiac disease and control group was observed (95% CI 0.5521 to 1.250, P< 0.05) According to our investigation maybe an abnormal expression of Lnc13 gene in blood of celiac patients use as a biomarker for the direction Prognosis, diagnosis, and treatment of Celiac disease.

  The aim is to detect volatile compounds and the antioxidant activity of the aqueous extract and the essential oil of Salvia multiculisvahlnative to Iran, and their efficacy in the oxidation stability of Sunflower oil. The extract and essential oil were prepared using the Percolation and by Clevenger apparatus through hydro-distillation method, respectively. The number of phenolic components of the extract and volatile components of the essential oil was identified by Folin-Ciocalteu and GC/MS method, respectively. In order to evaluate the antioxidant activity, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical trap method was used and the concentration that inhibited 50% (IC50) of free radicals were calculated. The antioxidant activity, in the delayed oxidative stress time of sunflower oil, was evaluated by measuring the oxidative stability index (OSI) and the active oxygen method(AOM). The number of phenolic compounds in the extract (24.23±2.11mg/g), was more than the essential oil. 21 components were detected in the essential oil, representing 97.1% of the total oil. The major compounds of it were 1,8-cineole (24.8%), camphor (17.9%), sabinen (8.6%), α-pinene (8.5%), and terpineol (7.1%). The IC50 of free radicals was obtained for extract and essential oil of 38.16±2.36 and73.14±1.23 μm/mL, respectively. Results show, the concentration of 250 and 350 mg/L of extract and essential showed the same effect with antioxidant synthetic of Grendoks 117 )200 mg/L). The use of extract and essential oil of Salvia multiculisvahl could be proposed as an appropriate antioxidant replaced with synthetic antioxidants.

 

The aim of the present study was to investigate the Short-term effect of grape seed extract (GSE) supplementation on the antioxidant capacity of non-athlete girls following an exhaustive aerobic exercise.
Materials and Methods
Sixteen non-athletes female students were randomly assigned into 2 groups of grape seed extract (three capsules of 100 mg per day for 4 weeks) and placebo (three capsules of maltodextrin 100 mg per day for 4 weeks). The first blood sampling was done from the brachial vein to measure the blood levels of the antioxidant enzymes mentioned; the second blood sampling was done after the 4-week period, Subjects then performed the Bruce aerobic exhaustive test protocol then, immediately after the finish of this test, the third blood sampling was done.

The results showed that in terms of the activity of SOD, GPX and POD enzymes, in baseline and post-supplementation, there was no significant difference between the two supplement and placebo groups. The activity of CAT enzyme after the supplementation period was significant between the two groups (p<0.05). The activity antioxidant enzymes in the GSE group in the baseline was significantly lower than after the supplementation period and after the activity (p<0.05). The mean of these enzymes after exercise was significantly higher than supplementation (p<0.05). Were significantly higher in the placebo group after the activity than the baseline and after supplementation (p<0.05).

According to the results, supplementation of GSE improved the antioxidant enzymes mentioned in non-athlete girls after the aerobic exhaustive exercise.

ntroduction
Today, synthetic Dye are extensively used different industries such as textiles, pulp, leather, food and cosmetic. Doys contained wastewater to prevent environmental consequences of must be treated effectively. The present work aimed to investigate the potential application of water treatment ferric sludge in the removal of Methylene blue (MB). The present experimental work was done on aqueous synthetic samples and in batch sorption mode. The effects of operating variables (pH, dose, contact time, an initial concentration of color), as well as kinetic and isotherm studies also included in this work. The adsorbent was prepared by drying the waste sludge treatment plant at 105C °. The maximum MB removal of was observed at a pH of 11, a contact time of 90 minutes, absorbance dose of 4 g/L and 25 mg/L of methylene blue. The conformity of kinetic and equilibrium data by psedu-second order kinetic and Freundlich models demonstrate that the adsorption occurs in heterogeneous and multi-layer. The experimental studies demonstrate the potential use of dried ferric sludge in methylene blue removal from contaminated samples.

  Breast cancer is one of the most common and worrisome diseases among women all over the world which becomes more difficult with localized relapses and metastases in this disease and the chance of survival is reduced. therefore the effect of various factors on the incidence of metastases can have an important role in the treatment and prevention of disease progression. In this study, 1959 women with breast cancer who referred to Omid Hospital in Mashhad from 2001 to 2011 were selected and evaluated. To analyze the data, after the imputation of missing data, the method of penalized profile likelihood was used and data analysis was done in the software R. The results of the analysis showed that family background, clinical stage, Her2 protein, and T (tumor size) variables had a significant effect on the incidence of metastasis in breast cancer patients. According to the results, the stage code and her2 variables, tumor size and family history in all conditions of analysis had a significant effect on the incidence of metastasis in patients with breast.