فهرست مطالب

Journal of Research in Dental and Maxillofacial Sciences
Volume:1 Issue: 3, Summer 2016

  • تاریخ انتشار: 1395/05/02
  • تعداد عناوین: 7
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  • M Vatanpour, A Falah Doust, G Shojaee, A Jamshidian *, A Farasat Pages 1-7
    Background and aim
    The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.
    Materials and methods
    In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.
    Results
    the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.
    Conclusions
    Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.
    Keywords: Cytotoxicity, Green tea extract, Inflammatory mediators, Ledermix® paste
  • S Sadatmansouri, N Ayubianmarkazi *, S Aghajeri, MR Delaram, B Iranpour Pages 8-13
    Background and Aim
    Several reports have been published on the successful application of various bone substitute materials (BSM). Appropriate physiologic and histologic characteristics and reactions of these materials against host cells are critically important. In this study, the biocompatibility of a new bone substitute material has been evaluated.
    Methods and Materials
     In this experimental In vitro study, the biocompatibility of silicate calcium phosphate, Bio-Oss and Osteon were compared by evaluation of cell viability and differentiation rate of human osteoblast-like cell line (SaOS-2). No graft material was used in the control group. Cell viability rate was evaluated by MTT test after 1, 3 and 14 days of incubation. Inverted Light Microscope and SEM were utilized for evaluation of cell morphology. MTT and cell morphology were analyzed by ANOVA test in all groups.
    Results
    Cell viability of the control group equaled 0.453±0.016, while in the test groups it equaled 0.453±0.016 for Bio-Oss, 0.439±0.011 for Osteon and 0.425±0.026 for silicate calcium phosphate. There was no significant difference between the control and the test groups. Spindle form was the dominant SaOS-2 cell morphology in all groups.
    Conclusion
    This study showed that calcium silicate phosphate has appropriate biocompatibility comparable with that of Bio-Oss and Osteon.
    Keywords: Saos-2, bone substitute, cell viability, cell morphology
  • SH Sakhdari *, L Hafezi, M Esmaili Pages 14-21
    Background and aim
    Mental nerve injury is one of the challenges of implant surgeries in the anterior mandibular region, which leads to complications such as paresthesia of the lip and chin. The present study aimed to evaluate the prevalence of the Inferior Alveolar Nerve's (IAN) anterior loop and mandibular incisive canal by use of CBCT.
    Materials and Methods
    In this descriptive study, 200 high resolution CBCT images of the patients referring to a private oral and maxillofacial radiology center were evaluated. The images were obtained by NewTom Giano unit and were measured in NNT viewer software. Reconstructed panoramic-like images were prepared, and upon the detection of the anterior loop, cross sectional slices were made and dimensions of the anterior loop were calculated by counting the cross sections. In addition, the maximum diameter of the incisive canal was measured, and the location of this diameter was determined on the cross sections relative to dental roots. Data were analyzed by Chi-Square test.
    Results
    In the 200 evaluated subjects, the prevalence of the anterior loop, with the minimum length of 3mm, was estimated to be 19% and the prevalence of the incisive canal was valued to be 87.5%. No significant correlation was detected between the presence of the anterior loop and age, gender, dental status and side of the jaw. Also, no significant association was detected between the presence of the incisive canal and age, gender and dental status (p=0/597,p=0/492,p=0/643) respectively. The maximum diameter of the incisive canal was 5.3 mm which was located between the first and second premolars.
    Conclusion
    Considering the results, it seems that evaluation and measurement of the length of the IAN's anterior loop and the diameter of the incisive nerve by CBCT are necessary steps to prevent nerve injuries during surgical procedures in the anterior mandibular region.
    Keywords: Mental nerve, Anterior loop, Inferior alveolar nerve, Mandibular incisive canal, Cone Beam Computed Tomography
  • F Hajifattahi, S Hosseini Jeddi, M Khatibi * Pages 23-27
    Background and aim
    reduction of dental plaque pH is an effective factor in the incidence of dental caries. One of the common methods for assessment of the cariogenic potential of food products is the study of plaque pH changes in the oral environment. The present study was performed due to the importance of dental plaque and its known complications and also the increase in consumption of industrial fruit juices which are encouraged nowadays as healthy drinks and also the positive effect of pomegranate juice on the amount of dental plaque which has been mentioned in the reports.
    Materials and methods
    This clinical trial was performed with crossover design. Complete prophylaxis was performed during the first session. Then, the participants were asked to refrain from oral hygiene methods for 48 hours and not to eat or drink for at least 2 hours before the experiment. The baseline plaque pH was measured, and afterwards 10 cc of fruit juice was kept in mouth for 2 minutes and then swallowed. Afterwards, plaque pH was measured at time intervals of 2, 5, 7, 10 and 30 minutes. After one week of wash out period, the participants were again evaluated by the same method and with the other type of fruit juice. The measurement of plaque pH was performed with microtouch method by use of Metrohm electrode. The data were analyzed by repeated measures ANOVA.
    Results
    pH in pomegranate juice group before fruit juice intake equaled 6.73± 0.24 and reached 5.57±0.34 at the fifth minute and finally reached 6.19±0.32 at the 30th minute (p˂0.01). Also, in orange juice group, pH before intake equaled 6.16±6.8 and reached 5.62±0.17 at the seventh minute and 6.15±0.2 at the 30th minute (p˂0.01). The maximum fall in pH for both fruit juices occurred at the fifth and seventh minutes. pH after consumption of both fruit juices began to increase from the tenth minute. These two fruit juices were not significantly different regarding plaque pH at the zero minute and at the time of maximum pH fall and at the 30th minute. (p˂0.08)
    Conclusion
    The results showed that plaque pH after consumption of both fruit juices falls below the critical level for seven minutes and this decline is similar for both fruit juices.
    Keywords: dental plaque, fruit juice, cariogenicity, pH, pomegranate, orange
  • A Azizi *, P Dabirmoghadam, F Keykha Pages 28-32
    Background and aim
    oral cancer is one of the most common cancers of the head and neck. Considering the high prevalence and mortality percentage of oral cancer and the significant advances in immunology and identification of different cytokines, the aim of the present study was to compare the concentration of IL8 in saliva of patients with oral squamous cell carcinoma (OSCC) and healthy subjects.
    Materials and methods
    in this case-control study, 20 individuals with a definite histopathology diagnosis of OSCC were selected as the case group and 20 healthy subjects were selected as the control group. The two groups were matched according to age, gender, cigarette smoking and alcohol consumption, medication consumption, periodontal disease (average CAL in first molars) and absence of systemic diseases. 5 ml of unstimulated whole saliva sample was collected with spitting method from each subject and the concentrations of salivary IL8 were compared between the two groups. Linear regression and t-test were used for statistical analysis.
    Results
    the concentration of IL8 equaled 741.82±722.81 pg/ml in the case group and 341.25±313.52 pg/ml in the control group and Mann-U-Whitney test showed that the concentration of IL8 in patients with OSCC is higher than that in the control subjects and this difference is significant. (p=0.049) According to regression equation, IL8 has a significant correlation with tumor size index (T). (p=0.011)
    Conclusion
    the results of this study showed that the concentrations of salivary IL8 were significantly different between the patients with OSCC and healthy subjects (p=0.049) and the concentration of IL8 has a significant correlation with tumor size (T).
    Keywords: Interleukin 8, oral squamous cell carcinoma, saliva
  • N Roghanizad, Z Khalilak, M Vatanpour *, S Haghi, P Alirezayi, Z Movassagh Pages 33-37
    Background and aim
    The purpose of this study was to evaluate the effect of autoclave sterilization on the fracture rate of ProTaper instruments (ex vivo).
    Materials and methods
    One hundred shaping and finishing ProTaper instruments were used in a crown – down technique for cleaning and shaping the mesial canals of extracted mandibular molars using 5.25% NaOCl for irrigation. In the experimental group, 50 files were steam autoclaved before each use. In the control group, the files were not exposed to the sterilization process. The working time was reported for each file. Independent t- test was employed to evaluate the statistical significance of the measured values.
    Results
    The instruments that underwent the sterilization processes did not show any statistically significant differences in comparison with those of the control group.
    Conclusions
    The results of this study showed that sterilization in autoclave in the presence of NaOCl did not lead to a decrease in survival time of the files.
    Keywords: Autoclave, Fracture, Nickel Titanium, rotary instrument, ProTaper, Sterilization
  • F Sarlati *, N Simdar, SH Razzaghi, R Shariatmadarahmadi, MR Shabahangfar Pages 38-43
    Background and Aim
    Toothbrushes cannot reach all interdental areas. Interdental cleaning is an important part of oral hygiene care. The purpose of this study was to compare the supragingival plaque removal efficacy of an interdental cleaning power device (Aquajet) and dental floss.
    Materials and Methods
    Thirty subjects were enrolled in this single-blind, split mouth clinical trial. All the subjects received both written and verbal instructions and demonstrated proficiency prior to the study. The subjects were asked to abstain from oral hygiene methods for 48 hours prior to the study. The subjects were scored using the Proximal/Marginal Plaque Index (PMI). Then, the four oral quadrants were randomly assigned to one of two treatment groups: One upper and one lower quadrant: Aquajet and the other two quadrants: dental floss. The subjects were observed to ensure that they have covered all areas and have followed the instructions. Afterwards, they were scored again using the PMI. The pre and post-cleaning plaque scores were evaluated using two-way repeated measure ANOVA.
    Results
    Both Aquajet and dental floss showed significant reduction of the baseline PMI in all dental areas (P<0.05), but the difference between the groups was not significant (P>0.05). Aquajet was significantly more effective than dental floss in reducing plaque on the mesial, mid-buccal and distal surfaces of upper first premolar and on the mesial and distal surfaces of upper second premolar and first molar (P<0.05).
    Conclusion
    The results proved that oral irrigation with Aquajet is as effective as that with dental floss in plaque removal, and that Aquajet had significantly higher plaque removal efficacy at hard-to-reach dental surfaces.
    Keywords: water flosser, periodontium, plaque index