مجله تنشهای محیطی در علوم زراعی
سال دوازدهم شماره 2 (تابستان 1398)

Five CMS lines and four restorer lines (tester) were crossed in a line × tester scheme in order to analyze general and specific combining abilities and gene effects in sunflower for some agronomic traits. Hybrids were evaluated in a randomized complete block design, with three replications under optimum and limited water conditions in the Agriculture Research Station, Khoy, during 2015 and 2016 growing seasons. Plant height, head diameter, seeds per head, days to beginning of flowering, days to maturity, proline percent, protein percent, and Chlorophyll b were found to be controlled mainly by dominance effects. CMS lines AGK30 and testers RGHK25 and RGHK50 turned out to be the best general combiners for seed and oil yield. The highest positive and significant GCA for plant height, head diameter, and the number of seeds per head were achieved for RGHK25. The hybrid combination of AGK44 × RGHK56 showed high specific combining ability for seed and oil yield. In the study of general combining ability, the AGK110 line has a positive and consistent general combining ability, and AGK2 has a negative and significant general combining ability with catalase . In the test of the testers, the tester (RGHK50) had the highest GCA positive and meaningful drug tester (RGHK56), which had a significant negative and significant combining ability with catalase . In the study of hybrid hybrid combining ability (RGHK46 × AGK260), the highest positive and significant positive and significant hybrid confinement (RGHK46 × AGK44) had the highest SCA negative and significant levels. In the RGHK46 × AGK260 hybrid combinability study, the highest positive and significant positive and significant hybrid confinement (RGHK46 × AGK44) had the highest SCA negative and significant effect on catalase identity. The AGK44 line has the ability to combine publicly and positively with AGK110 capability. The general combining ability was negative and significant with SOD. In the test of the testers, the RGHK 56 tester had the highest GCA positive and significant mean of the RGHK25 tester, which had a negative and significant general combining ability with superoxide dismutase. In the study of hybrid hybrid combining ability (RGHK50 × AGK260), the highest positive and significant positive and significant positive combining ability (RGHK50 × AGK44) had the highest SCA negative and significant values for SOD. In explaining the traits, plant height, Number of seeds per head and percentage of proline under stress conditions played a more significant role. Plant height and number of seeds per head were controlled by both growth and dominance effects. also In the study of general combining ability, the highest GCA positive and significant values were related to the AGK30 line and the most general negative and significant general combining ability was AGK2 line (Table 4-14). In the test of the testers, the tester (RGHK 46) had the highest GCA positive The drug tester (RGHK25) had the most significant negative and significant general combining ability with proline). In the study of hybrid combining ability (RGHK46 × AGK2), the highest positive and significant positive and significant hybrid confinement (RGHK25 × AGK260) had the highest SCA negative and significant values .

Rice is the staple food for approximately half of the world population. It is the basic food crop of Asia, providing over 30%-60 of the calories consumed in the region. Among abiotic stresses, drought is a major one, especially in arid and semi-arid areas. Evaluation of genotypes in drought stress conditions can increase our knowledge about the characteristics and traits that are effective in tolerance to water stress. Today, new graphical techniques are used to examine the effect of traits with respect to their interactions. One of these methods is GGE biplot. Although the GGE biplot was originally created to parse the data from multiple environments. However, this strategy, also, can be equally used for all types of 2-way data that assume an entry × tester structure. Considering the high water requirement of rice and lack of water resources the present study aims to investigate the relationship between traits and identify high yield tolerant genotypes in two conditions (flooding and drought) with 23 morphological traits in Gonbad-e-Kavous area designed. The materials used in this study, 263 genotypes of rice (Oryza sativa L.) were provided by IRRI (International Rice Research Institute) as a joint international project between the institute and Gonbad-e-Kavus University. The lines were evaluated in two separate experiments in the form of Latis design under normal conditions and drought stress in three replications. Each of 263 lines was cultivated in 2 meter and 25 centimeter meter square. 40 days after transplantation irrigation was cut and after 40 days, the subsequent irrigations were applied every 15 days. After complete sampling, the marginal effect was taken and 10 plants per line were harvested and transferred to the laboratory for measuring the traits. 23 morphological traits were measured and recorded according to the standard guidelines for evaluation of rice traits. The data obtained from this study was evaluated using SAS Ver.9.1 and GGE biplot (Yan, 2001) software. Due to the lack of significance between block changes in the Latis design, the data were analyzed using randomized complete blocks. The results of combined analysis of variance revealed that the effect of the environment, genotype and genotype × environment interaction were significant (p<0.01) for most measured traits. The biplot explained 41 and 46% of the total variation of standardized data for irrigated and rainfed (drought) conditions, respectively. Comparison of genotypes for the leaf rolling score showed that no strain was observed in non-stress conditions, but in drought stress conditions, leaf rolling score increased, depending on genotype susceptibility to drought stress. The highest rank (rank 7) belonged to genotypes 42, 44, 154, 194 and 199, and the lowest rank were genotypes 18, 63, 66, 77, 88, 98, 100, 102, 105, 107, 115, 167, 237, 310 and 312 respectively. The polygon view of Biplot from the interaction of genotypes in traits genotypes 118, 257, 88, 241, 289 and 154 introduced as an index genotypes for different traits in flood conditions. In drought stress conditions, numbers 63, 167, 201, 194, 199, 42 and 263 were identified as index genotypes. In both conditions, positive relationships were observed between traits. Comparison of genotypes based on plant yield with plant height and day to flowering showed that genotypes with higher grain yield had less flowering days and plant height. Therefore, it could be concluded that days to flowering and plant height had a negative correlation with grain yield. The results of this study showed that drought stress increased leaf rolling, increased number of days to flowering and reduced the majority of traits, especially plant yield. According to the results of graphite biplot analysis irrigated conditions, genotype HHZ 10-DT5-LI1-LI1 had the highest yield and stability and IR13F402 genotype had the lowest grain yield. In drought stress conditions, HHZ 3-SAL4-Y1-Y1 genotype had the highest performance and the second highest stability, HHZ 18-Y3-Y1-Y1 genotype was the most stable. Both genotypes were tolerant genotype and IR 11L412 genotype kknown as susceptible genotype.

 Growth analysis has been widely used to study crop and cultivar response to environmental conditions. Crop production is governed by the interception of radiant energy and the efficiency of converting this energy to dry matter. Numerous studies have shown a reduction in the leaf area index, crop growth rate, net assimilation rate and grain yield under drought stress conditions. Water deficit has a significant reduction on net assimilation rate, crop growth rate, total dry matter and grail yield. This has been attributed to promote leaf senescence and leaf abscission. Also water deficit stress has a significant effect on the reduction of net assimilation rate. This has been attributed to the phenomena of stomata closer and respiration increase. The aim of this study was to evaluate the effects of drought stress on growth indices and grain yield of wheat cultivar. This study was carried out in Tiran province, Isfahan, Iran during 2013-2014, as a split plot design in randomized complete block design with four replications. The main plots considered drought stress (irrigation after 70, 90 and 110 mm cumulative evaporation from class A evaporation pan), and sub-plots considered three wheat cultivars (Sepahan, Ghods and Pishtazs). Irrigation regimes applied from elongation phase. Plant samples were taken from 0.1 m-2 of all subplots with 20 days intervals and plants green leaf area were measured and oven dried at 75°C for 48 h plants weight mean were determined. The grain yield was measured at harvesting date. Statistical analysis of data was performed using the MINITAB statistical software and figures were drawn using by Microsoft excel software. The leaf area index in the irrigation after 110 mm, and in the irrigation after 70 and 90 mm cumulative pan evaporation treatments, in about in about 160 and 195 days after planting increased respectively, and was kept up for about 35 and 45 days at its higher surface in 110 mm evaporation and 70 and 90 mm evaporation respectively, then began to decrease. Delay in irrigation to 110 mm, plant had the lowest mean leaf area index and maximum leaf area index. Irrigation regime had a significant effect on total dry matter and delay in irrigation to 110 mm cumulative evaporation, significantly reduced total dry matter. The trend of changes in the total dry matter was more similar to the leaf area index. The leaf area index will be decreased by increasing irrigation intervals and that the total dry matter can also be decreased by reduction in photosynthetic capacity. The trend of changes in the net assimilation rate showed that in the irrigation after 110 mm, the net assimilation rate itself increased in about 165 days after planting and was kept up for about 30 days at its higher surface and in irrigation after 70 mm and irrigation after 90 mm the net assimilation rate itself increased in about 165 days after planting and was kept up for about 50 days at its higher surface, then began to decrease because of the extra leaves shading and of less absorption of the light by the leaves placed at the lower parts of the canopy. The trend of changes in the crop growth rate in the irrigation after 110 mm had been significantly lower than the irrigation after 70 mm and the irrigation after 90 mm. The trend of changes in the crop growth rate was more similar to the leaf area index but less resemble to the net assimilation rate. Irrigation regime had a significant effect on grain yield. Irrigation after 70 and 90 mm cumulative evaporation did not differ significantly for grain yield. Delay in irrigation to 110 mm cumulative pan evaporation, significantly reduced grain yield. Genotypes had a significant difference in gain yield. The Pishtaz cultivar had the highest grain yield. Result showed that irrigation after 70 and 90 mm cumulative evaporation did not differ significantly for leaf area index, net assimilation rate, crop growth rate and grain yield. Irrigation after 110 mm cumulative evaporation significantly reduced leaf area index, net assimilation rate, crop growth rate and grain yield. It was concluded that by irrigation wheat cultivar after 90mm cumulative pan evaporation, water could be saved by 22% with no significant loss in growth indices and grain yield under these conditions.

Under water shortage, applying and adjusting of some elements concentration such assilicon (Si) can be used as a strategy to reducethe negative effects of stress on wheat(Triticum aestivum L.) growth and enhanced plant compatibility.Some of the studies showed that exogenousSi application increased crop resistance to environmental stresses such as drought (Ahmed et al., 2011; Maghsoudi and Emam, 2016). Likewise, macro and micro nano fertilizers application caused an increase in crop yield. Therefore, this study aimed to investigate the effect of foliar application of nano and bulk Si dioxide particles on yield and yield components of wheat and also to determine the contribution of different sources in grain yield formation. In order to investigate the effect of foliar application of silicon on yield and redistribution of wheat dry matter under drought stress conditions, a field experiment was conducted as split plots based on a randomized complete block design with four replications at Ramin Agriculture and Natural Resources University of Khuzestan. Drought stress periods included three levels of normal irrigation (full irrigation throughout growing season or control), drought stress from stem elongation to flowering and drought stress from flowering to maturity were considered as main plot and foliar application of silicon dioxide levels in seven levels including non-foliar application (control), application of nano and bulk particles of silicon dioxide in concentrations of 50, 100 and 150 mg L-1 were considered as subplots. Data analysis was performed using SAS software and GLM procedure, and means comparison was carried out using a protected LSD method at a 5% probability level. Drought stress from stem elongation to flowering reduced the number of fertile tillers, number of spikelets per spike and number of seeds per spike, thereby reducing seed yield by about 35% compared to full irrigation conditions. Drought stress in the grain filling period resulted in a 10% reduction in wheat 1000-grains weight. Silica foliar application slightly reduced the effect of drought stress on grain yield.Wheat grain yield was significantly higher than other spraying treatments with the application of concentration of 100 mg L-1 of nano silicon particles (61.71 g m-2) and 150 mg L-1 of bulk silicon particles (572.73 g m-2). The efficiency of redistribution of dry matter in drought stress conditions from stem elongation to flowering (18.46%) was significantly higher than non stress conditions (14.07%) or stress induced from flowering stage to end of growth period (13.75%) Regardless of Silica foliar application treatments, redistribution of dry matter under full irrigation conditions was about 11% less than that of drought stress. The highest percentage of redistribution of dry matter from wheat grain yield was related to foliar application with concentration of 100 mg L-1 of bulk silicon particles under drought stress from flowering to the end of the growth period.
The contribution of current photosynthesis in grain filling in non-stress conditions (458.68 g m-2) was significantly higher than drought stress conditions from flowering stage to end of growth period (399.68 g m-2) and for this was also significantly more than that of the stress conditions from the stem elongation to flowering (261.98 g m-2). The contribution of current photosynthesis in grain filling in spray treatment with a concentration of 100 mg L-1 of nano silicon dioxide was the maximum (451.25 g m-2) and in control treatment was at least (311.11 g m-2).
It seems that nano silicon particles application caused an increase in plant tolerance to drought stress due toincreasing water balance, photosynthetic efficiency, and seedling growth (Hattori et al., 2005). In general, the present study showed that the presence of silicon and especially nano particles in drought stress has a significant role in improving yield and grain yield components of wheat as well as redistribution of dry matter. It seems that in order to reduce the negative effect of moisture stress during wheat growth season and increase grain yield and dry matter redistribution, application of 100 mg L-1of nanosilica particles is recommended.

In order to investigate the effect of drought stress on leaf area index, photosynthesis rate, chlorophyll, stomatal conductance and proline content in bean cultivars, a factorial experiment was conducted in a randomized complete block design with four replications. Treatments included drought stress in three levels, complete irrigation (control), irrigation cut at two weeks after emergence and irrigation cut at flowering stage and two bean cultivars were Talash and Khomain. Leaf area index, chlorophyll a and b, photosynthesis rate and stomatal conduction were decreased due to drought stress but amount of proline was increased. By applying drought stress at flowering time and two weeks after emergence compared to full irrigation (control), leaf area index was 30.57 and 55.96 percent, content of chlorophyll a was 23.1 and 56.05 percent and the content of chlorophyll b was 13.67 and 30.76 percent, photosynthesis rate was 13.22 and 26.73 percent, stomatal conductance was 31.65 and 50.25 percent decreased, respectively and amount of proline increased by 51.11 and 88.14 percent respectively.The highest and the lowest leaf area index were in complete irrigation (control) and Talash cultivar with 5.29 and in irrigation cuttings in two weeks after emergence and Khomain cultivar with 2.08, respectively.The highest amounts of chlorophyll a and b belonged to complete irrigation (control) and Talash cultivar with 0.213 and 0.118 mg/g fresh leaf weight and the lowest amount of chlorophyll a and b was related to irrigation cut at two weeks after emergence and Khomain cultivar with 0.132 and 0.76 mg/g leaf fresh weight, respectively. Generally, the negative effect of drought stress on chlorophyll a was more than chlorophyll b. Drought stress significantly reduced the rate of photosynthesis in bean cultivars.This decrease was observed in irrigation cut at flowering stage and irrigation cut at two weeks after emergence in comparison full irrigation (control) in the cultivar of the Talash was 12.99 and 24.50 percent respectively and in Khomain cultivar was 13.56 and 29.19 percent respectively.Stomatal conductance in complete irrigation (control), irrigation cut at flowering stage and irrigation cut at two weeks after emergence in the Talash cultivar was 0.432, 0.321 and 0.223 mol/m2.s and Khomain cultivar was 0.365, 0.233 and 0.154 mol/m2.s, respectively.The results of mean comparison showed that drought stress in each of the stages of growth reduced stomatal conductance in both cultivars. However, the percentage reduction in Khomain was more than the Talash.The content of proline in complete irrigation (control), irrigation cut at flowering stage and irrigation cut at two weeks after emergence in the Talash cultivar was 1.47, 2.30 and 2.83 mg/g fresh weight and in Khomein cultivar was 1.24, 1.78 and 2.25 mg/g fresh weight, respectively. drought stress in both cultivars increased significantly in proline content. According to the results, bean varieties had no significant difference in leaf area index and chlorophyll b content. In drought stress condition, Talash cultivar compared with Khomain cultivar showed less decrease in stomatal conductance and leaf area index and more had chlorophyll content and photosynthesis rate and it is recommended to cultivate in areas with drought stress.

Due to limited water resources in warm and dry areas, achieving maximum yield involves a lot of water consumption, Therefore, one of the important goals in the production of crops In such areas, the minimum water is determined to produce maximum yield In such areas determine at least water is that to produce Maximum performance (Paknejad et al., 2009). Based on climatic data Simulated in Iran For the next 30 years, drought is expected (Khazanedari et al., 2009). Plants are disposable to stress in nature, Drought stress is one of the most important limiting factors such as growth and production of plants have led to a reduction of more than 50% Average production of more crops across the world (Lata et al., 2011). Determine the appropriate planting date for plants is very important and it is an important factor which Affected plant physiological and morphological characteristics (Khodabande, 2009). So the purpose of this research is the effects of planting date and drought stress on grain and forage yield and some of the agronomic traits on foxtail millet. This research was carried out in crop year 2013 and 2017 in Zahak Agricultural Research Station. The experiment was conducted as split plot in randomized complete block design with three replications. The experiment factors included, S1-50% moisture evacuation (as control), S2- irrigation after 65% moisture evacuation and S3-Irrigation after 80% moisture evacuation and planting dates in four levels D1-23 February, D2- 5 March, D3- 20 March and 4 April Was considered as a sub-plot. Each experimental plot was planted in 6 lines 50 cm distance between lines and 5 meters long. Density was used of 10 kg / ha. The test plotting humidity was measured by the TDR regularly. The number of tiller per plant, leaf number per plant, plant height, number of days to reach, panicle length, 1000 grain weight, grain yield and fresh and dry forage yield were recorded. The statistical software MSTATC was used for statistical calculation. The Duncan test was used at 5 percent probability level to distinguish the averages. The results of analysis of variance showed that Planting date, drought stress and interaction of planting date in drought stress had significant differences on number of tillers per plant, leaf number per plant, plant height, number of days to reach, panicle length, 1000 grain weight, chlorophyll and proline concentration grain yield, fresh and dry forages. The highest plant height (83.16 cm), tiller number (2.11), leaf length (22.72 cm), Panicle length (14.08 cm), 1000 kernel weight (3.27 gr), grain yield (1521 kg/ha), fresh and dry fodder yield whit average (25225 and 6608 kg / ha) respectively, concentration of chlorophyll (5.83 mg/g) on sowing date March 15th and the highest concentration of proline (70.85 mg / g) was obtained on April 15. In 50% moisture discharge were the highest plant height (87 cm), number of tillers (2.37), leaf length (24.45 cm), Pancicol length (14.93 cm), 1000 kernel weight (3.25 g), grain yield (1677. In interaction March 15 and 50% moisture evacuation were the highest height (100/16 cm), number of tillers (3.16), leaf length (29.83 cm), panicle length (18.08 cm), 1000-grain weight (3.46 gr), fresh and dry forage yield (35550 and 9111 kg/ ha). According to the results, it can be stated that drought stress causing reduced production of grain and forage. But given the water shortage crisis in the country and the region, a slight reduction in yield on a scale with less water consumption should be put on the agenda of farmers. Planting of millet on sowing date on March 15 and 50% moisture discharge was caused the plant to be placed in the appropriate temperature conditions which can produce maximum yield. Finally, planting of the plant on March 15 and 50% moisture evacuation are recommended for maximum grain and forage millet yield.

The production of crops especially cereals in Iran is a challenge due to the presence of arid and semi-arid climates. Zeolite application is one of approach to ameliorate the effect of water deficit on crops. High water absorption zeolite is able to absorb water in the soil to a maximum level and maintain it for a long time within its network. Regarding the problem of water deficit and the necessity of managing agricultural production in conditions of water deficit and the possibility of using millet as a premature and compatible plant with most regions of Iran, this experiment was conducted out to evaluation of the effect of deficit irrigation and zeolite application on Proso millet. In order to study the influence of deficit irrigation and zeolite application on Proso millet, an experiment was conducted as strip block in randomized complete block design base, at Shahrekord University, in 2015. Irrigation regime gad four levels (supply of 100, 75, 50 and 25 percent of field capacity) and zeolite had three levels (0, 0.5 and 1 kg m-2). Plant water requirement was calculated by FAO Penman-Monteith equation. Irrigation regimes were applied at V4 stage (4th three foliate leaf has unfolded) and continued until the end of the growing season. Plant water requirement was calculated by FAO Penman-Monteith equation. Results showed that leaf area index (LAI) had sigmoidal pattern. The highest LAI (3.3) was recorded in 100%FC and one kg m-2 zeolite interaction. Also lowest LAI (1.1) was observed in 25% FC and 0 kg m-2 zeolite interaction. Results also showed that water deficit reduced dry matter accumulation but in each irrigation treatment increase of zeolite amount resulted in ameliorated the effect of deficit irrigation. Maximum and minimum of dry matter were recorded in 100% FC*1 kg m-2 zeolite and 25 % FC* 0 kg m-2 zeolite interactions respectively. The crop growth rate in all of the treatments increased until mid-growing season and then reduced. The deficit irrigation reduced membrane stability but zeolite application increased it. Membrane stability in 75, 50 and 25 percent of FC reduced by 23%, 66% and 57% respectively. Water deficit also reduced relative water content. RWC were reduced by 13%, 26% and 41% in 75, 50 and 25 percent of FC respectively. Stomatal conductance reduced by deficit irrigation but in each level of irrigation treatment the highest stomatal conductance was observed in 1 kg m-2 zeolite application condition. The grain yield of Proso millet was higher under 1 kg.m-2 zeolite application condition in all deficit irrigation treatments. The highest grain yield reduction compared to control treatment (100% FC and no zeolite application) was observed in 25% FC and no zeolite application condition. Finally results showed that however zeolite application under 100% FC condition had little effect but under deficit irrigation condition, zeolite reduced the harmful effects of deficit irrigation on studied traits and in this context uses of 1 kg.m-2 zeolite is recommended.

Oilseed rape is the most important breeding species in Brazil, and drought stress can affect its quality and yield. Water deficit is one of the most important factors limiting the growth and production of plants, and there is currently no practical way to increase the precipitations during the dry periods. Therefore, it seems that the best way to deal with drought is to use appropriate agronomic operations and to use cultivars with more tolerance to drought. In order to evaluate the qualitative characteristics of selected varieties of rapeseed, a factorial split plot experiment was conducted in a randomized complete block design with three replications for two years (1963-1953) in Karaj. In this research, the growing season was carried out in two dates (autumn and winter) and irrigation at three levels (normal irrigation or control, irrigation from later flowering stage and irrigation from the eating stage to the next) as a factorial as main plots and spring varieties Rapeseed consisted of Jerry, Julius, Zafar, Ridge S 003, Hayola 4815 and Zabul 10 as sub plots. Statistical analysis of SAS Ver 9 was used for analysis of variance. The Duncan multi-domain test was used to compare the averages. The results showed that palmitic acid in the second year had an increase of 8% compared to the first year. Among the seedlings, the highest average seedling planting season was allocated to 18.5%. Irrigation interruptions from post-harvest phases and discontinuation of irrigation from flowering stage resulted in 12 and 25% reduction of palmitic acid respectively. The results showed that the highest content of linolenic acid in the control treatments and RGS003 cultivars, irrigation cut off stage and Julius cultivar, and discontinuation of irrigation from flowering stage to Zafar cultivar, 6 and 76.7 percent respectively. The results showed that the highest mean linoleic acid was observed in control treatments, irrigation cut off from the jollowing stage and Julius cultivar, and irrigation cut from flowering stage to Heilah cultivar 4815, respectively, with mean 21.77, 19.66 and 17.68% respectively. . The results showed that in the control and irrigation intervals, the cultivar Julius with the levels of 66.29 and 64.16 percent showed the highest amount of oleic acid in the irrigation and after irrigation period, from the flowering stage to the Hyola 4815 cultivar with an average of 26.62%, the highest average. Effect of year, planting season, irrigation, year×irrigation, year×Planting season×irrigation, planting season×irrigation, cultivar, planting season×cultivar, irrigation×cultivar and planting season×irrigation×cultivar at 1% level and season effect×season Planting was also significant at 5% level on grain yield. Effect of year, planting season, irrigation, year×irrigation, year×Planting season×irrigation, planting season×irrigation, cultivar, planting season×cultivar, irrigation×cultivar and planting season×irrigation×cultivar at 1% level and season effect×season Planting at level of 5% and year effect and irrigation at 1% level were significant on seed yield. The results of the evaluation of traits during the two years of the experiment showed that Jolvie and Hayola 4815 had the highest grain yield under irrigation conditions, and also the Hyola 4815 cultivar had a good adaptation to drought stress conditions. Therefore, the cultivars could be obtained due to grain yield and higher oil yield under both drought stress conditions and without stress; for planting in regions of the same experimental region (Karaj), the probability of occurrence of moisture stress in the late stages of growth, Recommended. Also, the planting date of autumn increased the quantitative values of rapeseed quality indices.

Calendula (Calendula officinalis L.) which contains different types of phytochemicals including carbohydrates, fats, phenolic compounds, steroids, tripnoyids, tocopherols, carotenoids and quins that have beneficial effects on human health. Drought stress, caused by soil and atmospheric water deficiency, is one of the most significant environmental factors affecting plant growth and crop productivity in the majority of agricultural fields of the world. Nitrogen is one of the elements which its deficiency is noticeable in arid. semi-arid regions; because the amounts of organic matter which are the main source of nitrogen storage are very low in this region and in case of its existence, it decomposes immediately (Mengel, 2001). Nitrogen’s less or more availability to the plant causes some disorders in crop vital processes that may appear in the forms of abnormal growth and development, decrease in transpiration and even stunt in reproductive growth (Breemhaar, 1995).
The purpose of this investigation was study effects of irrigation regims and nitrogen on yield and yield components, essential oil medicinal plant Calendula. In order to study effects of irrigation regimes and nitrogen on yield and yield components, essence oil medicinal plant Calendula an experiment was conducted at Urmia Agricultural Station of Saat Lo at 2016 crop season. Field experiment was carried out by a split plot design based on completely randomized block design with three replications.
Treatments were four levels of irrigation levels (irrigation after 5, 10, 15 and 20 day) in main plots, and consumption of 0, 80, 160 and 240 kg/h nitrogen in sub plots. In this study, traits of number of heads per square meter, number of seeds per head, thousand kernel weight, biological yield, grain yield, oil percentage, oil yield and dry flower yield and flower essence were measured. Statistical analysis was performed based on the statistical model of the design used by SAS 9.2 software. Comparison of meanings of each trait was performed using SNK test at 5% probability level. Analysis of variance showed that irrigation levels had significant effect on all understudied traits also there were significant differences between nitrogen fertilizer levels on all traits except of thousand kernel weight. Also the effect of irrigation levels × nitrogen levels on number of seeds per head, grain yield, oil percentage, oil yield and essential oil percentage were significant.
Result showed, with increasing irrigation interval from 5 to 20 days, the number of heads per square meter, number of seeds per head, thousand kernel weight, grain yield, oil percentage, oil yield and dry flower yield decreased, and irrigation levels after 5 and 10 days were the highest values of the mentioned traits. In this research, irrigation level after 10 days had the highest number of seeds per head (22.91 grain), thousand kernel weight (11.19 g), dry flower yield (598.44 kg/h). Result revealed nitrogen fertilizer had positive effect on calendula growth characteristics
Although the highest amount of number of seeds per head (22.25), thousand kernel weight (10.52 g) and dry flower yield (575.12 kg/h) belonged to level of 180 kg/h nitrogen fertilizer. Among irrigation levels × nitrogen levels interaction treatments the highest value of number of seeds per head, (24.43), grain yield (1133.3 kg/h), oil yield (356.3 kg/h) and essence percentage (25%) was belonged to 10 days with 80 kg/h nitrogen fertilizer. According to the results of this study, it can be stated that nitrogen fertilizer application is effective when sufficient water is available to the plant, and increasing nitrogen levels when there wasn’t enough could have harmful effect on grain yield, oil percentage, oil yield and dry flower yield, oil percentage and flower essence in Calendula. Considering the limitations of water in the area as well as preventing environmental problems, irrigation intervals after 10 days and consumption of 80 kg nitrogen fertilizer in the region to achieve maximum qualitative and quantitative qualities in Calendula is recommended.

Coriander is an annual herb of the umbel family and is belonged from North Africa to south-western of Asia. Coriander is one of the important medicinal plant that used in the pharmaceutical industry and it mainly cultivated and widely distributed for the fruits. The dried fruits are widely employed as a condiment, especially for flavoring of sauces, meat products and bakery and confectionery items. Also, coriander fruits are as a source of essential oils and fatty oil. Water deficit stress is one of the most important factors limiting the growth and survival of plants in arid and semi-arid regions of the world. Water is a major component of the fresh produce and significantly effects on weight and quality of plants. Also, water deficit may cause significant changes in the yield and composition of essential oils in aromatic and medicine plants. So that, was reported that water deficit increased essential oil percentage in coriander but decreased essential oil yield. Iran with an average annual rainfall of 240 mm is included among arid and semi-arid regions of the world. Of the million hectares of cultivated region, only five millions are under irrigation because of intense water limitations. However, Iran is one of the world’s commercial coriander producers. Coriander has been cultivated for many years in different parts of Iran. Therefore, development of drought-tolerant cultivars with high essential oil yield is important in coriander. This research was conducted in order to evaluate the effect of drought stress on morphological, physiological and phytochemical characteristics of endemic coriander genotypes. F2 generations derived from half-diallel crosses of six endemic coriander genotypes including Isfahan, Hamedan, Bushehr, Mazandaran, Markazi and Alborz, together with their parents were evaluated in randomized complete block design with three replications in each experiment during growing season of 2016 in the research field of Tarbiat Modares University. Plants were treated with different levels of water treatment: well watered (WW), moderate water stress (MWS) and severe water stress (SWS). Data were collected on plant height, leaf number, branch number per plant, umbel number per plant, fertile umbel number per plant, fruit number per plant, thousand fruit weight, fruit yield, biological yield, essential oil content, SPAD chlorophyll content, relative water content and electrolyte leakage. The results of ANOVA and comparison of means indicated that the effect of drought stress was significant for all the studied characteristics. The results showed that by increasing drought stress the most studied traits decreased but the essential oil content significantly increased. The highest essential oil content was observed in moderate stress. Studies have, however, indicated that water stress have a number of positive effects on the biosynthesis of secondary metabolites such as essential oil content. Because in case of stress, more metabolites are produce in the plants and substances prevent from oxidization in the cells, but essential oil yield reduce under drought stress. This may because of the relationship between the amount of the essential oil and fruit yield that by progress in drought stress, increase in essential oil but decrease in fruit yield therefore essential oil yield is reduced. Also in the study was used of stress tolerance index (STI) as the best indicators for evaluating of tolerant to drought stress of coriander genotypes. The Mazandaran genotype and crosses derived from this genptype (Alborz Mazandaran, Markazi Mazandaran, Isfahan Mazandaran, Mazandaran Hamadan and Mazandaran Bushehr) were identified as drought tolerant genotypes. Generally, the results indicated that drought stress decreased all studied traits. Whereas essential oil content was increased under drought stress conditions. Also, The Mazandaran genotype and crosses derived from this genptype (Alborz Mazandaran, Markazi Mazandaran, Isfahan Mazandaran, Mazandaran Hamadan and Mazandaran Bushehr) as drought tolerant genotypes can be considered as donor parent which contains drought tolerance genes and could be used to improve coriander high essential oil yield in drought condition.
Acknowledgements: The authors thank from the Gene bank of the Seed and Plant Improvement Institute of Karaj, Iran for making available plant materials.

In order to evaluate the effects of different irrigation regimes and foliar spraying of chitosan on some qualitative and physiological characteristics of German chamomile (Matricaria chamomilla L.), this study was conducted based on randomized complete blocks design with factorial arrangement of treatment and three replications in Gonbad Kavous university research field, in 2014 growing season. Treatments included irrigation in two levels, irrigation after 60 and 100 mm evaporation from class A pan, and chitosan in five levels including non spraying of chitosan (spraying with distilled water as control (K1)), chitosan spraying as 125 mg/L after 60 days of planting (K2), 125 mg/L after 75 days of planting (K3), 250 mg/L after 60 days of planting (K4) and 250 mg/L after 75 days of planting (K5). Results indicated that utilization of chitosan under stress and under normal irrigation regime increased present of chamazulene, essential oil percentage and essential oil yield. According to our results, chitosan spraying in 125 mg/L 60 days after planting date under deficit water stress conditions improved peroxidase activity, chamazulene percentage and essential oil yield. In general, in order to increase the valuable medicinal products in German chamomile, spray of chitosan, as a natural material is important.

The Moldavian balm is herbaceous that originated in middle Asia and about 66 compound are identified in this plant. Application of growth regulator lead to increases of plant resistance against biotic and abiotic stress and is involved as a strategy to prevents adverse effects of environmental stress. According to semi-sensitivity of dragonhead plant to drought condition, its medicinal importance as well as, its industrial widespread use, this study was conducted to evaluate dragonhead essential oil yield and its compound under drought condition and also, to evaluate physiological aspect of drought resistance. In order to investigate the effects of salicylic acid and cycocel on the gas exchange on dragonhead in drought stress conditions a research was conducted in the spring of 2014 and 2015 at research farm of agricultural college, Zanjan University. The experiment was split plot with two factors of moisture levels and growth regulators in a randomized complete block design with four replications. The main factor is the moisture levels at two levels without stress (Complete irrigation until the end of the growth period), drought stress (Complete irrigation before start flowering and discontinuation of irrigation until the end of the growth period). Sub-substrate of different levels of salicylic acid (800 and 1600 μm), cycocel (600 and 1200 μm), and control (dissolve with distilled water), which applied for a single application time for 10 days before start flowering. The results showed that the interactions of the sampling stage in the year in hormone in moisture for intercellular CO2 concentration, transpiration, stomatal conductance and photosynthesis water use efficiency at probability level of 1%, it was quite significant. Intercellular CO2 concentration at the start flowering, full flowering and the end of flowering has a growing trend. And the rate of transpiration, stomatal conductance, photosynthesis and photosynthesis water use efficiency are decreasing. Cycocel treatment (600 μm) at the end of flowering stage increased intercellular CO2 concentration compared to other treatments. Salicylic acid (800 μm) treatment increased transpiration, stomatal conduction and photosynthesis compared to other treatments. The results of this study showed that with the application of drought stress, the gas exchange of the plant will change meaningfully. By closing the stomata, stomatal resistance increases and intercellular CO2 concentration increases and leads to a decrease in photosynthesis rate Reducing transpiration in drought stress is due to stomatal closure and stomatal conductance reduction. So that with intensity of draught stress, transpiration was decreased. The results showed that salicylic acid treatment can increase chlorophyll content by reducing the Chlorophyllase enzyme and increase the rate of photosynthesis and through increased stomatal conductance increases transpiration and it mitigates the negative effects of drought stress.The rate of photosynthesis had a decreasing trend from the start flowering, full flowering and flowering end. Salicylic acid treatment is likely to increase photosynthesis in the plant by increasing the leaf area, increasing chlorophyll content, preventing biosynthesis of ethylene and reducing the chlorophyllase enzyme. Cycocel treatments through increase stomatal resistance and stomatal conductance reduction, thereby reducing transpiration and decreasing the rate of photosynthesis, resulting in increased photosynthetic water use efficiency.

Drought stress is one of the most important stresses that affects plant growth and yield (Redd et al., 2004). Drought stress results in the formation of reactive oxygen species by the partial regeneration of oxygen in the processes of photosynthesis and respiration by increasing the transfer of electrons to oxygen molecules (Asada, 1999), Plants have different mechanisms to reduce the harmful effects of activated oxygen species. In recent years, due to the changing weather conditions, drought stress has become more severe. Thus, the study of mechanisms that enable plants to adapt to drought stress can ultimately help to produce resistant plants in arid and semi-arid regions (Hassani et al., 2003). In addition to improvement of nutrition, the fungus symbiosis is able to reduce the negative effects of environmental stresses on host plant in various ways, such as increasing the levels of antioxidant enzymes and maintaining chlorophylls (Zarea, 2012). The negative effects of environmental stresses on host plants are reduced. In this study, the effect of mycorrhizal fungus on photosynthetic pigments and the characteristics of antioxidant enzymes of chicory under drought stress were investigated. In order to study the effect of mycorrhizal fungus on photosynthetic pigments and the antioxidant specification of chicory under drought stress, the factorial experiment was conducted in a completely randomized design with three replications in a Greenhouse of Agricultural Research Center of Zabol University in 2016. Treatments were four levels of drought stress (control), 70, 50 and 30% of crop capacity and inoculation with mycorrhizal fungi at two levels of inoculation and uninoculation. Before planting, 50 g of mycorrhizal fungus was added to the soil of each pot. From the beginning of the fifth week, drought stress was applied to weights In the end, vegetative parameters including fresh and dry weight of root and shoot were measured. Arnon method (Arnon, 1976) was used to measure chlorophyll and carotenoid. The enzyme gyAgol peroxidase (Cam-mada and Nelson Shspra, 1999), the polyphenol oxidase enzyme (Janovitz-Klapp et al., 1990) and the measurement of the catalase enzyme by Beers and Sizer (1952) Made.   The highest fresh and dry weights of shoot Plant in 90% stress and inoculated with Glomus Fascollaria fungus treatments were obtained and the least amount was obtained in drought stress 30% agronomic capacity and non inoculation with Glomus Fascollaria fungus (Table 2). The highest fresh and dry weights of roots were obtained in stress 90% and inoculation of Glomus Fascollaria fungi and its lowest in drought stress 30% of field capacity and non-inoculation with Glomus Fascollaria fungus (Table 2).The results showed that in the interaction of Glomus Fascollaria fungus treatments and drought stress, the highest levels of chlorophyll a, chlorophyll b, carotenoids and total chlorophyll were obtained in 90% stress (control) and inoculated with Glomus Fascollaria fungi and the lowest levels of chlorophyll a, chlorophyll b, carotenoids and Total chlorophyll was obtained in drought stress 30% of field capacity and uninoculation with Glomus Fascollaria fungus (Table 2). The highest effect of interaction between fungal treatments and drought stress in catalase, polyphenol oxidase and Guayaquil peroxidase enzymes was observed in drought stress 30% of field capacity and inoculation with fungi and its lowest level in 90% stress (control) and uninoculation with fungi Came. In corn plants, inoculation of Glomus Fascollaria fungi increased chlorophyll content, and attributed an increase in chlorophyll content to increased nitrogen uptake by the mycorrhizal system (Tang et al., 2009). It was reported that chlorophyll content in Citrus tangerine seedlings mixed with fungi under full irrigation conditions had a significant difference with chlorophyll content in non-fungal seedlings, and in drought stress conditions, chlorophyll content in plantlets containing fungi was higher than that of plants There was no fungus (Wu and Xia, 2006). In studies on zoo, the highest activity of catalase was found in leaves of plants inoculated with Glomus Fascollaria fungi under drought stress than uninoculated plants (Suleimani and Pirzad, 2016).Based on the results, with increasing drought stress, the amount of photosynthetic pigments, fresh and dry weight of the shoot and fresh weight and root crop decreased. Inoculation with Glomus fascollaria during drought stress reduced drought stress and increased photosynthetic pigmentation, fresh and dry weight gain of the shoot and increased fresh and dry weight of roots. The inoculation of chicory with Glomus fascollaria fungi under drought stress conditions by stimulating the activity of antioxidant enzymes such as catalase, polyphenol oxidase and guaiacol peroxidase prevents the oxidative effects of ROS. Thus, the use of mycorrhiza fungi improves the physiological function and plant tolerance Chicory was drought stressed

In order to investigate the influence of drought stress and also arbuscular mycorrhiza (AM) fungi inoculation on growth, yield and yield components of chickpea (Cicer arietinum L.), a pot experiment was conducted using a factorial based on completely randomized blocks design with three replications. The factors were contained three levels of irrigation [satisfactory irrigation (100% irrigation), moderate irrigation (70% irrigation) and deficit irrigation (40% irrigation)] and mycorrhiza inoculation (non-inoculated and inoculated with Glomus etunicatum, Glomus versiforme, and Glomus intraradices). Results showed that drought stress had significant effects on evaluated characteristics. As with decrease of the available water of plant, height of the flowering branches, number of flowering in a branch, dry weight of root, total dry weight, weight of pod, number of pods per plant, grain weight and yield decreased. Also, AM fungi inoculation had significant effects on evaluated characteristics. Plants inoculated with AM fungi had higher growth, growth and yield than non-inoculated plants under drought stress and non-stress conditions. Thus, it could be concluded that at drought conditions AM fungi are able to enhance the growth and yield of chickpea under drought stress condition through enhancing nutrient and water uptake.

Black cumin (Nigella sativa L.) as an annual medicinal plant which belongs to the Rununculaceae family is suitable for semi-arid regions. The researchers reported many medicinal properties for this plant such as bloating reduce, pain reduce, anorexia reduce and anti-cancer. Water is one of the most important environmental factors in plant growth that its reduction limiting crop yield. Drought stress can be effective on vegetative growth, flowering, pollination and fertilization, fruit formation and yield and reduces each of these factors. In recent years, the use of plant growth promoting rhizobacteria through different mechanisms against Salt and Drought Stresses can increase plant tolerance to environmental stresses. The aim of this study was to investigate the effect of plant growth promoting rhizobacteria on yield and yield components of Black cumin under water deficit stress To evaluate the effect of plant growth promoting rhizobacteria on agro-morphological traits of Black cumin under water deficit stress, an experiment was done during 2014-2015 growing season at the research field of the faculty of agricultural, Shahrekord University as split-plot factorial in a Randomized Complete Block Design with three replications. The main factor including water deficit in three levels (100, 75 and 50 present of water requirement) and the sub factor was PGPRs at seven levels (control, Bacillus sp. Strain A, Bacillus amyloliquefaciens, Bacillus sp. Strain B, Azotobacter chroococcum, Pseudomonas putida and Azopirillum lipoferum). The amount of irrigation water was calculated using plant water requirement. Before the final harvest, to measure traits, five plants were randomly taken from each plot and yield and yield components was recorded. SAS and Excel softwares implemented for statistical analysis and the means were compared using LSD test. The results showed that by increasing water deficit stress the amount of the number of branch, Plant height, seed number per capsule, the number of capsule per plant, 1000 seed weight, biological yield, grain yield and harvest index was decreased. So 50% of water requirement treatment cause to decrease 18.4, 28.4 and 12.3 percent in biological yield, grain yield and harvest index compared to 100% % of water requirement. the use of bacteria significantly improved all these traits compared to non-inoculated. Among the bacterial treatments the maximum and minimum seed number per capsule, 1000 seed weight, biological yield and grain yield obtained in Bacillus amyloliquefaciens treatment and non-inoculated, respectively. Also, the results indicated that the interaction effect between water deficit stress and bacteria inoculation, except the number of branch and Plant height, were significant on all of traits. B. amilolykofosins application under 100% water requirement treatment had the highest seed number per capsule, 1000 seed weight, biological yield and grain yield (increased by 38.7%, 17.13%, 24.8% and 35.2%, respectively) as compared to non-inoculated. So, plant growth promoting rhizobacteria by different mechanisms such as phosphate solubilization, dinitrogen fixation, ACC deaminase and antifungal activity, IAA and siderophore biosynthesis are responsible for the plant growth promotion and increased yield under different environmental stresses. The results of this study showed that increasing water deficit stress causing significant reduction of all traits. However, the use of bacteria reduced the effects of stress and increased these traits compared to non-inoculated at all water deficit stress levels. Also among bacterial treatments, Bacillus amyloliquefaciens, Bacillus strain A and strain B had the most effect on stress improvement.

The Salvia is a large genus with about 58 species from Lamiaceae family in Iran. It has been well-known in pharmacopeia that has been used with special attention in the ancient era. The Essential oils, terpenoids, derivatives of phenolics acids and flavonoids are the main secondary metabolite constituents in Salvia species. The Pharmaceutical properties of the Salvia plants are mainly due to antioxidant activities of their secondary metabolites. It has various applications in Pharmaceutical, foods, hygienic-cosmetics and perfume industries as well as utilized in beverages as flavor.
The species of Salvia officinalis is a perennial medicinal herb from Lamiaceae family. The origin of salvia species has been reported in the northern Mediterranean and North Africa zones. The most important constituents of the essential oil are included: Tujan (30-50%), Cineol (10-15%), Camphor (6-10%) and Borneol (6 to 14%). Recently, it has been reported that the essential oil of the S. officinalis, especially some of its compounds, including Cineol, Tujan, Camphor, have an antimicrobial, antioxidant and anti-cancer properties. Drought is the most harmful factor of crops and medicinal plants production in the arid and semi-arid eras which has a negative impact on all aspects of plant growth and production. Also, in the plants exposed to severe soil dry the photosynthetic apparatus activities were damaged or impaired, leading to a decrease of photosynthetic capacity and lipid oxidation is increased in these conditions. Therefore, the use of mechanisms that lead to losses of this tension can be useful. One way to deal with the devastating effects of drought is the use of up to date and modern technologies such as Nano science.Nano-titanium dioxide has beneficial effects on the growth, physiological processes and activities of the plant metabolism.This study was to evaluate the effect of titanium dioxide spray on some physiological characteristics and activities of antioxidant enzymes sage was conducted under drought stress. This study was conducted in 2017 at the Faculty of Agriculture, University of Zabol a factorial in a completely randomized design with three replications. The experimental treatments was included drought stress (irrigated in Field capacity, depletion of soil water content up to 50% and 75% of FC condition) and foliar Nano Titanium dioxide spray (Control, 50 and 100 mg.l-1). Physiological traits (Chlorophyll a, b and T. chlorophyll, Total phenol, Proline) and activities of antioxidant enzymes (Peroxidase (POD), Ascorbate peroxidase (APX), Guaiacol peroxidase (GPX), Superoxide dismutase (SOD) and Catalase (CAT) were estimated in this experiment. Based on the results, increasing of stress severity reduced some traits, So that the soil’s water content lose to the 75% of the field capacity led to decrease in dry matter, Chlorophyll a, b and T.Chlorophyll of the plant’s leaf. However, total phenolic content, Proline concentration, the Peroxidase (POD), Ascorbate peroxidase (APX), Guaiacol peroxidase (GPX), Superoxide dismutase (SOD) and Catalase (CAT) activities were increased. Also, the results of simple effects of foliar application levels showed that by increasing the concentration of foliar application, the traits were increased, so that The highest Chlorophyll a and relative water content of leaf was obtained in plants treated with 100 mg.l-1 TiO2 and on the other hand, the results of reciprocal effects showed The highest phenolic content, Proline concentration, and the activities of POD, APX, GPX, SOD and CAT were obtained in plants subtended in depletion of soil water content up to 75% of FC and sprayed with 100 and mg.l-1 TiO2. According to the results it could be stated that due to the small size of TiO2 particles and easier penetration into the leaf it can affects growth and photosynthetic properties of sage and by reduced the lipid peroxidation, through increasing the antioxidant defense system activities and the content of osmotic regulators (Proline) of the plant and consequently, leads to cells’ structures stability against water stress.

Drought is a meteorological event occurring due to the absence of rainfall in a period of time. With the occurrence of drought stress, the water available to the soil is reduced, but the loss of water through evapotranspiration increases continuously. Drought can be effective in reducing the rate of germination and germination percentage, and inadequate The necessity of moisture for germination in soil layers and consequently drought stress in seedling stage is one of the important factors in the lack of desirable seedling establishment in arid regions. Considering the importance and role of medicinal plants in different industries, as well as lack of resources Water and abundance of arid regions in the country, it is necessary by applying techniques on the seeds of these plants, proper management of the production and cultivation of these valuable species, and Increase their performance. The purpose of this study was to investigate and use nanosilver particles in increasing or improving the indices of germination of two important species of Thymus. Seeds of Thymus genotypes Thymus daenensis Celak and Thymus vulgaris L. from Isfahan Seed Packers Company were obtained. This study was carried out in a factorial experiment in 2009 in a completely randomized design with three replications in University of Kashan. Drying treatments were considered at four levels (0, 0.3, -0.6 and -0.9 MPa) and treatments of silver nanoparticles with four concentrations (0, 10, 20 and 30 mg / l).Data were analyzed by SAS statistical software (ver.9). First, the normalization of all the data from different germination traits, including germination percentage, germination speed, root length and stalk length and seedling strain, were evaluated using Kolmogorov-Smirnov test. . Then analysis of variance of data and comparison of means were done using Duncan's test at 5% level. The results showed that the interaction of species, drought stress and nanoparticles on germination rate and shoot length were significant at 5% probability level. The highest germination percentage was observed in both species in control treatment and with increasing dryness, germination percentage, germination rate and seed vigor decreased in both species. Thymus daenensis Celak had a higher rate of germination than the species of Thymus vulgaris L. The species of Thymus vulgaris L in comparison with the species of Thymus daenensis Celak has a relatively low level of roots in terms of the length of its cultivation. On the other hand, silver nanoparticles in non-dry conditions in Thymus vulgaris L.caused an increase in the length of the root, but in the species of Thymus daenensis Celak it was not observed. In general, silver nanoparticles in all traits, with the exception of stem length, had only a mild-to-moderate-particle dose of 30 MPa. Under drought stress conditions, silver nanoparticles in all traits, with the exception of stem length, had only a slight increase of -0.3 MPa, which results in the ability of silver nanoparticles to reduce the negative effects of drought stress below -0.3 MPa, especially Proof of nanoparticles with a concentration of 30 mg / l. Due to the favorable germination and rapid seedling growth, better seeding and reduced competition with other plants, the use of nanoparticles in these two important species of thyme can be a good way to produce these two species in drought conditions. In the life span of the plant, rarely favorable conditions are provided in terms of different environmental characteristics, and as a result, the actual germination in the field is less than the predicted value in germination tests. Therefore, seed vigor index can be more effective in determining the seed germination rate in the field. However, increasing the index of seed vigor with the presence of silver nanoparticles at low levels of dryness has been proved in these two species, but to check other characteristics of the above plants, we need additional testing in field conditions.

Wheat is the most important crop in the country. However, a significant portion of this product is grown and produced in saline lands. In Khuzestan province, when we move from north the south, the land salinity is increased due to low slope, heavy soil texture and high water deficit. So, the major areas in Ahvaz, Shadegan, Abadan, Khorramshahr, Mahshahr, Dashte Azadegan, Hoveizeh and even in parts of Shoshtar, Ramhormoz and Behbahan are confronted with this limitation. Estimates showed that at least 400,000 hectares of south of province require underground drainage. Many studies have shown that the application of growth regulators such as salicylic acid can increase plant tolerance during abiotic stresses, especially salinity stress. Therefore, this experiment aimed to investigate and compare the effects of different methods of salicylic acid hormone application on quantitative, qualitative and biochemical parameters of wheat in drained and non-drainage lands. This experiment was carried out in order to evaluate the effects of different methods of applying salicylic acid on quantitative, qualitative and biochemical parameters of wheat in drained and not drained lands, based on combined analysis with randomized complete block design with three replications. The first factor was two levels of drained lands (first experiment) and not drained lands (second experiment) and the second factor was different methods of salicylic acid application, which was 1- no spraying (control), 2- soaking of seeds 3- spraying at the beginning of tillering, 4- soaking of seeds + spraying at the beginning of tillering, 5- spraying at the end of tillering, 6- soaking of seeds + spraying at the end of tillering. In this experiment, salicylic acid was used at a concentration of 1 mM. Soaking of seeds in solution was done for 6 hours and washed with distilled water and then dried. Also, spray application of salicylic acid in different growth stages, was performed by motor sprayer. Determination of grain yield from one square meter was done from each plot after removal of margins. To determine the number of spikes per square meter, the number of spikes in harvesting area was counted and averaged in each plot. To determine the number of grains per spike, 10 spikes were selected randomly from all harvested spikes and their grains were counted and their mean was considered as grains number per spike. To determine the number of spikelets per spike, 10 spikes were cut in each plot randomly and the number of spikes was counted and their mean was considered as spikelets number per spike. 1000-grain weight was calculated based on the total weight of two 500 grains samples at the harvest stage. Chlorophyll index was determined by using chlorophyll meter at grain filling stage. Different concentrations of proline, in mg/g fresh weight, were measured by Bates method and wheat protein was measured by digestion method by Kejeldal device. The results showed that the effect of land type on spikes number per square meter, grains number per spike, spikelets number per spike, 1000-grain weight, grain yield, chlorophyll index, proline and grain protein percentage were significant at 1% probability level. The effect of salicylic acid levels on all traits was significant at 1% probability level. The interaction of land type and salicylic acid in terms of protein percentage was significant at 5% probability level and in term of spikes number per square meter, spikelets number per spike, 1000-grain weight, chlorophyll index and proline content were significant at 1% probability level. The maximum protein percentage (14.36%) was related to the no spraying of salicylic acid (control) in non-drainage conditions  and the minimum in drained conditions (10.6%) was related to soaking of seeds + spraying at the beginning of tillering. The maximum and minimum grain yield in drained and non-drained lands by 3308 and 2017 kg.ha-1 was observed, respectively and the maximum and the minimum grain yield in different methods of salicylic acid application was in seed soaking + spraying at the beginning of tillering and without salicylic acid application by 3636 and 1759 kg.ha-1, respectively. In general, the results showed that the effect of salicylic acid on drained lands increased the yield parameters and on not drained lands by reducing the effects of salinity stress and improving the damage caused by it could be effective in increasing of yield. However, in this condition, the lowest yield qualitative was observed.

Basil (Ocimum basilicum L.) is a medicinal plant, annual herb of the Lamiaceae family. It is extremely important because of the large amounts of essential oils in the vegetative organs and the diversity of the secondary compounds in the essential oils. Salinity is one of the important factors limiting the growth of plants and causes osmotic stress and ionic toxicity. Drought stress reduces the rate and percentage of emergence and finally lead to delay seedling establishment. Therefore, tolerance to stress in the early stages of plant development is very important and seedlots that have the ability to germinate under stress conditions, have a successful establishment, have a good density and have a high crop yield. In this regard, the aim of this study was to identify tolerate basil accessions to salinity and drought stresses at the emergence stage. This experiment was conducted as a factorial based on a completely randomized design in 2017. Twenty basil accessions were planted under control, salinity and drought conditions in the greenhouse and seedling emergence were recorded daily. In this study rate and percentage of seedling emergence, mean emergence time (MET), and emergence energy (GE), genetic and phenotypic variations, coefficient of genetic and phenotypic variations, and broad sense heritability for the evaluated traits were calculated. The results showed that all of the accessions in the control conditions had a high emergence percentage of salinity and drought conditions. Under stress conditions, some of the accessions had high emergence percentage; the results of analysis of variance showed that the difference among the accessions for emergence percentage and rate, mean emergence time and emergence energy in the control conditions. However, most of these differences were not significant under drought and salinity stress conditions. This indicates the high susceptibility of the Basil, which, in the conditions of salinity and drought, has not been much disagreement among the accessions. In control conditions, the highest percentage of emergence was observed in Isfahan 2 accessions. Regarding the traits of emergence, the least time to emergence, the greatest energy of emergence of Shiraz accession was better than other accessions. In the conditions of control, the percentage of the accessions and the rate of emergence were higher in drought stress than in other accessions. In other words, the difference among accessions in terms of drought stress was lower. In the conditions of salinity stress, a similar trend was observed with drought stress, and the difference between the accessions in these conditions was less than the control conditions. In drought stress, the average time to emergence of Birjand (purple) in 7 days, and in Soiis and Gorgan in 11 days, it can be concluded that in drought stress of Birjand (purple) accession, less time needed for emergence and has better performance in dry soils, although its difference It was not meaningful with some other accession. With the stress, emergence energy decreased. The differences in emergence energy among accessions were significant in the control and drought conditions, but it was not significant in salinity conditions. In control conditions, the highest emergence energy was related to the Shiraz accession and the lowest amount of emergence energy was the Soiis accession. Also, under drought stress, the lowest emergence energy was associated with the Napoleonic accession and most related to the Malayer green accession. The results showed that Isfahan2 and Shiraz under control condition, Isfahan 2, 3 and Birjand under drought stress and Pishva (Green) and Zahedan under salinity conditions, had better seedling emergence and establishment than the other accessions. In this research, genotypic and phenotypic variances, genotypic and phenotypic variation coefficients and broad sense heritability were estimated for the traits. Finally, there was a good variation in tolerance of different accessions to salinity and drought stresses, which can be used to breed new basil cultivars tolerated to salinity and drought. It should be noted that further studies on essential oil content and yield of these accessions should be studied in future studies in order to make a better selection.

The cultivation of crops in most parts of the world, in addition to competing with different weed species, is affected by a variety of environmental stresses, including salinity stress. The changes in the growth rate and production of crops under the influence of salinity stress and competition with weeds have been extensively studied. However, what is currently less investigated is the competition of several species of weeds with crops and the competition of crops with weeds under salt stress. Therefore, the aim of this experiment was to evaluate the competition between tumble pigweed, common purslaneand and common millet under soil salinity stress. This study was conducted as a factorial experiment based on a randomized complete block design with three replications. Experimental treatments consisted of two rates of soil salinity (3 and 10 dS.m-1), and different ratios of interference of the three species (tumble pigweed, common purslaneand, millet) including 0-0 -100, 0-25-75, 12.5-12.5-75, and 25-0-75, 50-0-50, 25-25-50, 0-50-50, 75-0-25, 37.5-37.5-25, 0-75-25, 0-100-0, 50-50-0 and 0-100-0. The desired density in each pot was constant and equal to 8 plants. After preparing the soil and passing through a 2 mm sieve, fertilizer and saline solutions were added to the soil and the soil was transferred to 5 kg pots. Then all three plants were planted and attained to intended density at the four leaf stage. At the end of the experiment, each plant species was separately harvested from each pot and were weighed after complete drying. Finally, for analyzing the results of the experiment, relative yield indicators, total relative yield (Land Equal Ratio), relative crowding and agressivity indices were used. The results showed that increasing soil salinity decreased the dry matter produced by common millet, tumble pigweed and common purslane by 19.5, 4.7 and 27.8 percent, respectively. Comapring the relative yield of millet with those of tumble pigweed and common purslane and also both weeds together by different ratios of planting showed that these values were more than predicted ones under either 3 or 10 dS.m-1 salinity level, which indicates the weak competitive ability of these two weeds against millet. The results of the relative crowding index of species showed that in the ratio of 75% of millet and 12.5% tumble pigweed and 12.5% of common purslane, the greatest rate of relative crowding index was achieved for millet under salinity level of 3 dS.m-1. Also, the greatest relative crowding index of tumble pigweed and common purslane under either salinity level was observed with the cultivated ratio of 75% for both weeds and 25% for millet, suggesting an increased competitive ability of these two weed species through increasing their density even under a higher level of soil salinity. By evaluating the agressivity index between tumble pigweed and purselane, it was found that this value was negative for purselane under the salinity level of 3 dS.m-1. Therefore, it seems that tumble pigweed is more competitive than the common purslane where both weed species grow together. In general, the results of this study showed that in conditions of salinity of 3 and 10 dS.m-1, common millet had more competitive ability than the two studied weeds, but with increasing salinity level, competitive ability of millet was reduced, while it increased weeds competitiveness. Therefore, in the situation of high weed densities, we may expect a greater damage to crops under salinity stress.

To assess the effects of sodium exclusion on leaf chlorophyll content and biomass production, three bread wheat cultivars with contrasting of sodium concentration and different salt tolerance were grown at 2 salinity levels (0 and 150 mM NaCl) under hydroponics and greenhouse conditions in 2017 year. Salinity decreased yield, shoot dry weight, shoot K+/Na+ ratio and chlorophyll content and increased shoot Na+ compared to control. In this experiment, shoot K+ was not affected by salinity. Shoot Na+ concentration was more in salt tolerant cultivar (Kavir) than Mahdavi and Tajan under salinity stress and there was no relationship between Na+ exclusion and salt tolerance in cultivars. Leaf Na+ concentration were much lower in Mahdavi than two other cultivars. Chlorophyll content showed the most decreases in Tajan (75%) than Kavir and Mahdavi under salinity stress. Yield and shoot dry weight was reduced to the same extent (40% and 37% respectively) by 150 mM NaCl in each cultivar, despite greater chlorophyll content by Kavir cultivar. Results showed that Kavir had the highest ability to tolerate high leaf tissue concentrations of Na+. Despite different sodium concentration, the reduction in shoot biomass under salt stress was the same for all cultivars. It seems that the major effect of salinity on shoot biomass and yield was due to the osmotic effect of salt, not due to Na+-specific effect within the plant.

Legumes are one of the most important crops to be effective in amending and fertilizing soils. Among legumes, bean, lentil, chickpea, broad bean and pea are most susceptible to salinity (Cobely, 1996). Abiotic stresses affect different aspects of plant growth, such as reduction and delay in germination, decrease in development rate, decrease in plant organs growth, decrease in plant life duration and finally decrease in dry matter production. Among abiotic stresses, salinity stress is considered to be the most influential type of stress in the production of legumes in the world and can greatly reduce production on many arable lands. One of the primary effects of salinity is the reduction of water content of plant tissues. Reducing water availability is due to an increase in the osmotic potential of salt in the root zone (Ghanbari et al., 2016). Seed priming is one of the methods for improving seed quality in which the seeds are exposed to water under controlled conditions, so that different metabolic and physiological activity occur at different levels of moisture inside the seed for germination before the radicle emergence and then seeds are dried to reach initial moisture (Farooq et al., 2006). To evaluate the effect of salinity and seed aging on germination characteristics of bean seeds, Sadri variety, a factorial experiment was conducted in a completely randomized design with four replications in Agriculture Laboratory, Faculty of Agricultural Sciences, University of Guilan in 2014. The treatments consisted of two groups of seeds (natural and aged) and five salinity treatments (0, 2, 4, 6 and 8 dS m-1). Before testing, seeds were disinfected with 10% sodium hypochlorite solution for 15 minutes (Ghanbari et al., 2016) and washed three times with distilled water. For aging, dried beans were exposed to a temperature of 41 °C and high relative humidity (100%) for 72 hours in an oven placed in a desiccator. Then, 25 seeds were put in each sterile petri dish with filter paper. To each petri dish, five milliliters of NaCl solution with potentials of 2, 4, 6, 8 dS/m were added and distilled water was added as control treatment. After applying the treatments, the petri dishes were covered with parafilm and were placed in the germinator at 25 °C in the dark for 8 days (Ghanbari et al., 2016). The results showed that there were significant differences between treatments for all the variables. In addition, the interaction of seed aging with salinity stress had highly significant difference at the probability level of 5% of plumule fresh weight and there were significant differences at the probability level of 1% for radicle and plumule dry weight, allometric index, seedling tissue water percentage and seed vigor, while regarding of means and percentage of germination and plumule length were not observed significant differences. Research on the effect of seed aging and salinity stress on bean germination characteristics showed that increasing the aging period and increasing levels of salinity stress negatively affected the bean germination characteristics and by the effect on plant water relations, toxicity due to ion accumulation, plasma reduction, molecular structure change of nucleic acids and decreased activity of enzymes eventually led to decreases in germination indices (Ghanbari and Karamnia, 2016). Increasing levels of salinity stress and aging the seeds reduced the activity of germination, mean time and germination percentage, and seedling growth. the results of seed deterioration can be destroyed the buildings the cell membrane with attack free radicals produced due to lipid peroxidation over the period deterioration noted. Therefore, seed aging and increase levels of salinity stress on the germination and its related factors in bean seed had a negative impact and through its impact on plant water relations, toxicity of ion concentration, reducing the plasma membrane integrity, change the molecular structure of nucleic acids and reduction of activity of enzymes eventually reduced the germination indices.

Salinity stress is a serious threat of plant growth and production over the world. Soil salinity is the major problem, which affects the yield of wheat crop. It is commonly observed in arid and semi-arid regions of the world (Atlassi et al., 2009). Crop growth reduction due to salinity is generally related to the osmotic potential of the root-zone soil solution. The relative salt tolerance of wheat crop is 7.0 dS∙m−1 and its yield decrease is 25% at 9.0 dS∙m−1 (Kramer and Amtmann, 2012). The reduction in growth and yield varies between cultivars (Sultana et al., 2000). The varietal differences in salinity tolerance that exist among crop plants can be utilized through screening programs by exploiting appropriate traits for salt tolerance. The recently developed cultivars form a much diversed genetic base and may therefore possess a wider range of salts stress tolerance. In order to study the effects of salinity stress on grain yield, yield components, morphological traits and concentrations of Na+ and K+ of eight wheat cultivars and elite lines, two separate experiments in randomized complete block design with three replications were conducted at two places of Amirabad (saline condition) and Mohammadieh (none saline condition) in 2009-2010. Four elite lines including MS-88-8, MS-88-16, MS-88-17 and MS-87-8 and four salt tolerant cultivars including Ofogh, Arg, Bam and Kavir investigated. The first experiment conducted at the Research Farm of Birjand University located in Amirabad region which texturally the soil was sandy clay loam, with pH=8.2 and EC=13.69 ds.m-1. The second experiment conducted at the South Khorasan Agricultural and Natural Resources Research and Education Center located in Mohammadieh region which texturally the soil was loam, with pH=8 and EC=4.79 ds.m-1. Investigated traits were including flag leaf area, spike length, peduncle length, plant height, number of grain per spike, thousand grain weight, biological yield, grain yield, harvest index, Na+ and K+ concentrations and stress indices. Data analyses were performed using two-way analysis of variance with SAS 9.1. Means of treatments were compared according to Duncan test at the 5% level. Results showed that salt stress led to significant reduction on all morphological traits and had the most effect on plant height. Salt stress had no effect on number of grain per spike, 1000 grain weight, and Na+ and K+ concentration in shoot. Biomass and grain yield in salt stress condition decreased 38.2 and 44.5 percent, respectively. MS-87-8 and Ofogh with grain yield of 4.02 and 3.72 ton/ha were better than Bam and Kavir checks and same as Arg. The best indices for selection of tolerant cultivars were HARM, STI and GMP, which Arg and MS-87-8 identified as the most tolerant based on them. The results of this experiment showed that salinity significantly reduced grain yield, biomass and morphological traits of wheat cultivars and promising lines. Meanwhile, Arg cultivar and then MS-87-8 line had the highest grain yield and identified suitable for saline condition. Due to the presence of salinity resistance genes, they can be used for breeding of cultivars with high potential yield and also to cultivate in saline conditions.

Salt stress is one of the important reasons of different problems in agricultural productions. Also, because of competition between sodium (Na+) with essential cations that necessary for cellular function, salinity cause to limit in absorption of mineral contents and growth reduction. One way of exciting salt from soil profile is using organic amendments such as biochar that recently has been extremely considered as a result of the weather modifications and soil management. Due to the characteristics of organic matter biochar, in this research the effect of biochar on growth traits, sodium (Na+) and potassium (K+) concentration of summer savory leaf under salt stress resulting from irrigation with NaCl were investigated. In order to investigate the effect of biochar on growth characteristics, sodium (Na+) and potassium (K+) concentration of summer savory ( Satureja hortensis L.) leaf under NaCl stress, a pot experiment was performed as factorial based on completely randomized design with four replications in greenhouse condition at Horticultural Research Station, Ferdowsi University of Mashhad, Iran, in 2017. The factors of the test included three levels of biochar (0, 1 and 2 % w/w of soil of each pot) and irrigation with salty water in three levels of salinity (0, 40, 80 mM NaCl). In order to produce biochar, the woods of mulberry (Morus alba) three put in electrical furnace with 530 C temperature for 14 h. Then the biochar crushed in very small pieces, sieved and added to the soil. The growth parameters included height, number of branch let, stem diameter, number of nodule, stem fresh and dry weight, leaf fresh and dry weight were measured by common methods. The amount of Na+ and K+ from the leaf samples were also measured. Data analysis were done with Minitab 17 software. The results of analysis of variances showed that the interaction effects of salinity and biochar treatments on number of branch let, stem diameter, number of nodule, stem fresh and dry weight, leaf fresh weight, Na+ , K+ and K/Na ratio in P<1% and on height and leaf dry weight in P< 5% were significant. The mean comparison of data indicated that the highest plant height (32.17 cm), number of branches (18.92), number of nodule (10.25), stem diameter (11.88 mm), stem fresh (1.75 g/plant) and dry (0.6 g/plant) weight, leaf fresh (3.44 g/plant) and dry (0.82 g/plant) weight were observed at the treatment of 2% w/w biochar without salinity were observed. Furthermore, the highest Na+ (1.69% leaf dry weight ) and K+ (3.39% leaf dry weight) were observed at 80 mM NaCl without using biochar and the treatment without salinity and biochar (control( , respectively. With increasing salt concentration, the K/Na ratio decreased and at the highest salt concentration (80 mM) reached to the lowest amount (1.71%). Salinity stress through limitation in mineral element, disrupting ionic balance, deficit in available water of plant and toxicity of mineral element cause to reduce cell growth. Competition between Na+ and K+ is one of the important physiological mechanisms of salinity stress resistance. K+ deficit is one of the events that occur due to the competition between Na+ with K+ for absorption in the roots. Therefore, it seems that in this experiment application of biochar resulted in increasing the tolerance to salinity by decreasing absorption of Na+ with plant and increasing K/Na ration. The results of this study showed that salinity cause to reduce the growth of summer savory and application of biochar in this condition cause to balance the harmful effects of salinity. In the treatment 80 mM NaCl without using biochar, the highest reduction in studied traits was observed. Moreover, adding biochar by absorbing Na+ of soil cause to reduce Na+ of plant. Due to the results of this research, correct application of biochar could be a suitable biological way for increasing the tolerance of plants to salinity

To plant breeding in relation to high temperature tolerance, and for determining the differences between resistant genotypes, several indices in the basis of crop yield in normal and stressed conditions were be introduced. With regard to importance of genetic breeding for higher yield and stability in exposure to terminal heat stress in warm region of Iran, and little studies about tolerance of safflower cultivars, this study was carried out by measuring tolerance and sensitivity indices. Safflower is one the most important oil crops in regions that have dry and warm winters like to Khuzestan province of Iran. This crop are relatively tolerant to environmental stresses. But it is reported there are significant differences between different cultivars. The study was conducted to evaluate the difference between safflower cultivars in Ahvaz region. To comparison of safflower cultivars by indices of tolerance and sensitivity to terminal heat stress, present study was carried out in Ramin Agriculture and Natural Resources University of Khuzestan, at 2016-2017. A split plot design in the basis of Randomized Complete Blocks Design (RCBD) with four replications was used for the study. Experimental factors was included four sowing date (11 November, 2 December, 5 January and 3 February) were placed in main plots, and safflower cultivars (sina, goldasht, soffeh, Esfahan local (kooseh) and faraman) were placed in subplots. In this study, several indices with regard to researcher’s reports were calculated and used for determining the difference between safflower cultivars. Statistical analysis was done using by SAS v. 9.1 software. The results of statistical analysis showed that investigated cultivars had significant differences in terms of Stress Sensitivity index (SSI), Tolerance Index (TI), Productivity Mean (PM), Harmonic Mean (HM), Yield Stability Index (YSI), (GPM), Stress Tolerance Index (STI), Stress Damage Index (SDI), and Modified Stress Tolerance for appropriate conditions (MSTI1), Modified Stress Tolerance for no appropriate conditions (MSTI2), and Yield Reduction Index (YRI). However, differences between cultivars in terms of Ranking Index (RI) and Yield Index (YI) were not significant. Therefore, STI was the best index for evaluating tolerance and sensitivity of safflower cultivars to terminal heat. On the basis of STI, most tolerate and sensitive cultivars were goldasht and Esfahan local (kooseh), respectively. The results showed that safflower cultivars had significant differences in terms of tolerance indices calculated in the study. In the basis of Stress Tolerance Index (STI), goldasht cultivar had highest tolerance (lowest sensitivity) and local Esfahan cultivar (kooseh) had lowest tolerance (highest sensitivity) to terminal heat stress.
Generally, in regions exposed to environmental stress, best cultivars are the cultivars that in normal conditions have high yield and in stressed conditions have no significant reduction f yield (group-A in Fernandez classification). In this study, stress tolerance index (STI) had good ability for determining of these cultivars.

Calcareous soils, show serious limitation in accessing of nutritional elements. Majority of Iran’s soils are placed in arid or semi-arid regions and they are also in Alkali and lime conditions. As this calcareous soil mostly placed in hot climate conditions, naturally they are poor in organic matter. Humic materials are main and persistence components in organic matter and they could increase the growth of plants as well as the nutritional elements sorption. Humic acid is a natural organic Polymer Which as a result of the decomposition of soil organic matter, pit and lignin will be created and they will improve yield and quality of plant products. Humid matters will increase the resistance of plants in inappropriate environmental conditions. In spite of extended research on extraction and use of humic acids in plant nutrition, there is a limit attention in comparison effects of humic acid extracted from different sources on plant yield in calcareous soils. Hence the aim of this research is the effects of extracted humid acids from different sources on sunflower yield in soils with permanent lime stress. This research performed in greenhouse of university on sunflowers. The method was completely randomized factorial design with 3 replications in 2014-2015. Treatments were included: Soil application and foliar application with 5 sources of humic acids (Biochar 400 degree, biochar 200 degree, Sludge, manure and control). Humic acid treatments both in Soil application and foliar application were performed in 4 stages. Moreover of plant traits, (including Number of leaves per plants, stem length, fresh and dry weight, ash weight, Chlorophylls a, b, total and carotenoids and concentrations of phosphorus and potassium elements), there are also physical and chemical properties of extracted humic acids (including humic acid percentage, carboxylic groups, total acidity, OH-phenolic groups, and E4/E6, E3/E5 ratios) that they were measured. Studying results of the physical and chemical properties of extracted humic acids, shows that the highest amount of extracted humic acid was observed in sludge treatment which had the highest proportion among other resources. Also according to the results, the highest amount of phenol groups, carboxylic groups and total acidity were observed in humic acid extracted from manure. The highest ratio of E3/E5 (the largest molecular size of humic acid) is related to humic acid extracted from biochar 400 degree. The smallest molecular size (E3/E5) was observed in the treatments of humic acid extracted from biochar 200 degree. The results showed that the humic acid extracted from any sources, had a positive effect on all traits of the plant and increased all traits significantly. In Foliar application treatments, The highest increase in leaf number, chlorophyll a, b, total and carotenoids and potassium was observed; where Soil application of humic acid sources showed the highest increase in stem length, dry weight, ash weight and phosphorus of plants. Humic acid extracted from sludge had a significant effect on stem length (63%), chlorophyll a (47%) and phosphorus (1.25 times). Also in compare with control, the humic acid extracted from biochar 200 degree, had a positive effect on the number of leaves (59%), fresh weight (60.23%) and dry weight (1.21 times), chlorophyll b (1.13 times), total (35%) and carotenoid (62%) and potassium (36%). Interaction effects of biochar 200 degree, had a positive effect on traits like the number of leaves (61%), dry weight (1.4 times), chlorophyll a (62%), total (64%), potassium (45%); also biochar 400 degree, had a positive effect on traits like chlorophyll b (1.5 times) and carotenoid (50%); soil application used biochar 200 degree had a positive effect on traits like fresh weight (75%); sludge had a positive effect on stem length (58%) and phosphorus (1.75 times) and manure had a positive effect on traits like ash weight (3.22 times), and this things all together had a positive effect on plant growth and yield. In general, according to the results, the foliar application of Humic acid extracted from biochar 200 degree had the best performance on increasing the yield and its components, which may be attributed to the small molecular size of the extracted humic acid from biochar 200 degree.