نشریه بیولوژی کاربردی
سال دوم شماره 3 (پیاپی 7، پاییز 1391)

Iron oxide nanoparticles have been used as contrast agents in the MRI and in thermal therapy for cancercells. However, their, their adverse effects on human health have not been fully investigated. In thisexperience the effect of iron oxide nanoparticles on morphological and pathological changes of livertissue, kidney and spleen tissue in rats was investigated. Mice in Three experimental groups werereceived iron oxide nanoparticle (20, 50 and 150 μg/kg) which was dissolved in 1 ml of distilled water bygavage tube daily and for fifteen days. The control group was received 1 ml of distilled water daily.Results showed morphological and pathological changes in the tissues. Meanwhile results showed thatthis change was depended to the dose. In liver, tissue necrosis, inflammation and increased number oflymphocytes was observed. Meanwhile port area was extensive. Necrosis of kidneys and destruction ofbowmen9s capsule and glomerular atrophy was observed. Meanwhile atrophy of spleen tissue was severeand loss of red pulps and white pulps increased. According to the results it seems that Iron oxidenanoparticles have toxic effects on the liver, kidney and spleen tissues that this effects increases withincreasing dose.

In this research specific growth and chlorophyll of Spirulina platensis has been measured in differentorganic media (fruit died skin, cattle manure and poultry manure) and convey media as control in 15pptsalinity, 25°C temperature, pH=7, 3000 lux light, photoperiod 12h and aeration for 24h by triplicate inlaboratory. Cell concentration has been determined by spectrophotometer in 620nm wave length forspecific growth and 664,647 and 630nm for chlorophyll amount diurnal. According to results, the highestspecific growth rates was 0.0799 division day-1 at poultry manure while cattle manure and convey wereLowest 0.0023 division day-1 without any significance difference(p

In this research, antioxidant activity of the peel of fruits from Solanum melongena L., cultivated in Iran,was evaluated for the first time. The results, exhibited significant antioxidant activity in the studiedsample. To evaluate quantitative and qualitative effects of microwave irradiations on the extractedsample, yields and antioxidant activities of the extracts, prepared by soxhlet and microwave assistedmethod were compared. Antioxidant activity was evaluated via DPPH method with BHT as standard.The results showed that both extracts have high potential to scavenge free-radical compared with BHTstandard antioxidant; these active antioxidants are extracted under microwave irradiations with highyield; these irradiations have no destructive effect on antioxidant compounds of the plant, during a shorttime, and also yields of extractions of the mentioned methods are not highly different. these active antioxidants are extracted under microwave irradiations with highyield; these irradiations have no destructive effect on antioxidant compounds of the plant, during a shorttime, and also yields of extractions of the mentioned methods are not highly different.

This study was carried out to investigate the effect of clinoptilolite (the best known and most abundantnatural zeolite) of Qom province on performance, carcass characteristics, ileum microbial population,ileum acidity, litter microbial population and litter moisture of broilers. This experiment was carried outusing a completely randomized design with 5 treatments, 4 replicates and 20 experimental units eachcontain 20 male chicks of Ross-308.At the first week of experiment, chickens were reared under a same condition and afterwards they weredivided into experimental units randomly. All chickens were reared on litter and received ad libitum dietsand water from 8 to 42 days of age. Experimental treatments were as follow: 1) Diet free of zeolite andbentonite (control group), 2) Diet with 1 percent of commercial zeolite (CZ), and 3-5) Diets with 0.5, 1and 1.5 percent of zeolite of Qom province (QZ) respectively.During the experimental period, feed consumption and body weight of chickens were measuredweekly, and on day 32nd, 1 sample was gathered from each pen to determine the litter moisture andlitter microbial population. At the end of the experiment, two birds from each pen were slaughtered torecord the carcass percentage and the relative weight of thigh, breast, heart, liver and small intestine.Also all contents of ileum, sent to the laboratory to determine pH and microbial population of ileum.All samples were cultured in BA and EMB.Results indicated that feed consumption of broilers did not significantly differ among allexperimental groups...

The purpose of this study was to investigation and compares the developmental potential ofisolated blastomeres from two cell embryo by four different isolating methods.Material&

In order to remove Zona Pellucida and isolating blastomeres, physicochemicalmethods including exposure to tyrode acid, Hyaluronidase, EDTA and mechanical force were used. Theblastomeres were cultured. Number of degenerated and abnormal embryos counted and diameter andtotal cell number of blastocysts were measured.

Degenerated rates of blastomeres were 42.85, 57.5, 62.48 and 61.60 in mechanical, tyrode acid,EDTA and Hyaluronidase groups, respectively. It was lower in mechanical group than the others groups(P< 0.05).

In mechanical group, there were lesser negative influences on developmental potential ofisolated blastomeres from two cell mouse embryos to blastocyst stage.

In mechanical group, there were lesser negative influences on developmental potential ofisolated blastomeres from two cell mouse embryos to blastocyst stage.

Milk is considered a nutritious food because it contains several important nutrients including proteinsand vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcusaureus. In this study, 123 milk samples of 123 cows from four farms of different geographic locations inQom were collected from March until November 2011. The samples were cultured on Blood agar andbacteria were identified by standard methods. On the basis of cultural and biochemical properties, 26Staphylococcus aureus isolates obtained from 123 milk samples. Among a 26 isolates, 9 isolates (7.31%)were positive coagulase and 12 isolates (9.75%) were positive DNAase.Milk is considered a nutritious food because it contains several important nutrients including proteinsand vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcusaureus. In this study, 123 milk samples of 123 cows from four farms of different geographic locations inQom were collected from March until November 2011. The samples were cultured on Blood agar andbacteria were identified by standard methods. On the basis of cultural and biochemical properties, 26Staphylococcus aureus isolates obtained from 123 milk samples. Among a 26 isolates, 9 isolates (7.31%)were positive coagulase and 12 isolates (9.75%) were positive DNAase.