نشریه بیولوژی کاربردی
سال سوم شماره 1 (پیاپی 9، بهار 1392)

The prevalence of diabetes in word is rapidly rising.Stress oxidative is risk factors in diabetes. . If untreated, it is complications ,such as atherosclerosis , ophthalmic complication ,retinopathy and cardiovascular complications, nephropathy, disability and death.Satureja rechingeri essence has antioxidant effects.The aim of this study was effect Satureja rechingeri essence on treating diabetes in diabetic male wistar rats. The study was carried out on 30 male rats. Male rats were divided into three groups randomly,group one as control, group two diabetic untreated, group three diabetic treated with Saturejarechingeri essence by injection 100 mg/kg ,i.p .Diabetes was induced in the second and third groups by injection 60 mg/kg ,i.p of streptozotocin .After 3 weeks ,the serum levels of glucose،triglyceride (TG), cholesterol (C), LDL ,VLDL ,HDL of all groups were analyzed. The serum levels of glucose, triglyceride (TG), cholesterol(C) low density lipoprotein (LDL),very low density lipoprotein (VLDL) decreased in treated groups significantly.The levels of high density lipoprotein (HDL) increased in treated groups significantly. This study showed that Satureja rechingeri essence effect on treating diabetes in diabetic rats.

Genus Vibrio is a gram negative, curved, motile and non spore former bacteria. Natural habitat of these bacteria is environments such as sea and rivers .However, some of Vibrios are pathogen ,but most of them are non pathogen for human being. However recently chemotaxis behavior of these bacteria has been considered as invasive factor.This behavior helps the bacteria to move toward of suitable conditions to increase their survive in the environment .The present study conducted to evaluate the chemotaxis behavior of Vibrio spp. isolated from Persian gulf to perform the study 50 samples were collected and 2 Vibrio spp. were isolated and phenotypic and genoty identified. Chemotaxis behavior of the isolates was evaluated using diffusion agar method and (BM) device. the chemotaxis behavior of the isolate was evaluated against Phenylalanine, Cysteine , lysine ,Glycine, Galactose, Maltose, Rhamnose and Trehalose .The result optained indicated that 2 species of Vibrio alginolyticus and Vibrio mimicus were isolated. Vibrio alginolyticus exhibited positive chemotaxis behavior against lysine ,Glycine, Maltose, Trehalose, Galactose and negative chemotaxis behavior to Phenylalanine, Cysteine and Rhamnose. Vibrio mimicus showed positive chemotaxis against Phenylalanine, Cysteine ,Maltose, Rhamnose and Trehalose and negative chemotaxis behavior to lysine ,Glycine and Galactose.

Infections caused by vancomycin resistant enterococci (VRE) is increasing all over the world Ast.mqavmt glycopeptide antibiotics the bacteria associated with genes van A, B, C, D, E is the effect of the antibiotics in the wall of the bacteria. Most cases of VRE are Enterococcus Fyshyvm inherently resistant to tobramycin gentamicin so as aminoglycoside of choice for combination therapy with bactericidal agents used Recently it was reported that many VRE very high resistance to all aminoglycosides and even gentamicin shows. With the development of resistant strains of enterococci, so this research is to find new antimicrobial agents against vancomycin resistant enterococci is done.After sampling the soil and surrounding villages Qom, isolation and identification of Streptomyces strains on the environment was ISP4 After separation and purification, isolates were vancomycin resistant enterococci in vitro against MHA, BHI, Hicky trenser medium was granted a total of 125 isolates, 5 isolates growth inhibitory activity against VRE showed. Strain that most halo of inhibition against enterococcal isolates showed biochemical and morphological traits and 16s rRNA sequences were identified based on identification results showed that superior strains similar to the 100% of the Bashd.nalyz Streptomyses tendae ATTC 19812 GC-Mass...

Lichens are a set of algae and fungi, form long times lichens are used in traditional medicine, therefore, identifying the lichens and their ability to produce antimicrobial product is important.

In the present study effect of four lichen types, which has been collected from around Tabriz and identified by Botanical Center of Iran, Tehran were evaluated. Then effect of watery, alcholoic and acetonic extract of each lichen were evaluated on 3 pathogenic bacteria belong to: Escherichia coli ( PTCC 1395), Shigella dysenetriae (PTCC 1188) and Salmonella typhi ( PTCC 1609) using well diffusion agar method and their MIC ( 0.5- 0.0156 g/ml) were determined. In addition, the presence of phytochemical compound such as: flavonoid, terpenoid and tannin were evaluated

The results obtained from this study indicated that out of all extracts, watery extract had no effect on bacteria but ethanolic and specially acetonic had more effect on bacteria such as: E. coli and S. typhi. On the other hand, results showed that lowest MIC belong to acetonic extract of Staurothele on E.colli . Additionally, presence of flavonoid and trepenoid confirmed in all of lichens and presence of tannin was confirmed only by Staurothele..

According to the results obtained from this study could be concluded that Staurothele had best antimicrobial effect and could be use in further studies.

The aim of this study was to evaluate the pattern of antimicrobial metabolites that are capable of inhibiting the growth of Lactobacillus indigenous intestinal bacteria are pathogenic. For this study, Lactobacillus strains were isolated from 50 samples of vegetables from the production of antimicrobial metabolites were evaluated. The anti-microbial isolates producing metabolites, phenotypic and molecular identification (16srRNA). The production phase was analyzed for each isolate. Among these strains, 2 Lactobacillus species able to produce antimicrobial metabolites were much more powerful than others. Continuing this research chemical structure of antimicrobial metabolites by HPLC and SDS-PAGE methods were evaluated.The results showed that two samples of Lactobacillus plantarum and Lactobacillus rhamnosus 20205DSM 1136JCM, are able to produce antimicrobial metabolites. These isolates the bacteria Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and fungi Aspergillus Niger and Candida albicans showed growth inhibitory effects.The results of HPLC and SDS-PAGE indicated a similar structure Lactacins and organic acid compounds produced by strains that evidence is to introduce these strains as probiotics.

Factor analysis was conducted over two dietary patterns were identified. Western dietary pattern with high consumption of sweets, jams and preserves, mayonnaise, soft drinks, salty snacks, solid fats, high fat dairy products, fried potatoes, Ama’ and viscera, meat and processed foods, tea and coffee , a healthy diet with a higher intake of refined grains and cooking oils, legumes, nuts, fruits, poultry and fish, whole grains, eggs, vegetables and low-fat dairy Bvd.ps adjusting for the impact of possible turbulence earn higher scores the Western pattern diet significantly (P <0.05) were associated with increased risk of prostate cancer (odds ratio 2.33; 95% CI, 9/4 to 1/1) in front earn a high score of a healthy diet significant relationship (P <0.05) with a reduced risk of prostate cancer (OR = odds of 25/0, 95%, 53/0 to 11/0). lower risk of prostate cancer.People with a diet rich in refined grains close to the Western dietary pattern, red and processed meat and high-fat and fried food more risk of prostate cancer versus those who risk their diet is closer to a healthy diet lower risk of prostate cancer.