نشریه بیولوژی کاربردی
سال هفتم شماره 2 (پیاپی 26، تابستان1396)

Diabetes is the most common and important diseases in the world, and diabetic patients are at high risk of infection. Due to the high prevalence of diabetes and risks of urinary tract infections, ccauses of urinary tract infection and the proper way to treat it acutely felt

In this study, 353 urine samples of diabetic patients cultured and by using biochemical tests were examined. After DNA extraction, PCR test for definitive diagnosis of bacteria and the antibiogram test was carried out.

Urinary tract infection in diabetic patients 28.3% was reported. Asymptomatic bacteriuria 22.1% and symptomatic bacteriuria 6.22% was reported. The most common bacteria that cause urinary tract infections in patients were respectively Escherichia coli, Klebciella pneumonia, Proteus, Staphylococcus saprophyticus, Acinetobacter, Citrobacter, Enterobacter, Pseudomonas aeruginosa and Providencia.

Given that bacterial population of urinary tract infections in diabetic patients, similar to non-diabetic patients, so antibiotic treatment in diabetic patients is alike with and non-diabetic patients.

With the increasing use of environmental pollutants removal adsorption process, the selection of a suitable material in terms of technical and economic catchy title, was one of the concerns of researchers in this field. The aim of this study was to determine the amount of phenol and aniline use of aqueous solutions. In this study, Applied according to intended purposes in laboratory scale batch system, as an adsorbent modified dates of 4/0, 6/0, 8/0 and a gr,The effect of changes were studied in the concentration of phenol, vanillin, ph, absorbent concentration and contact time. All tests analysis were used excel performed according to standard methods of water and sewage systems and software for data. The results showed that ash core of high efficiency removal and aniline, phenol and phenol and aniline adsorption efficiency by increasing adsorbent dose, are directly linked and the absorption efficiency of a heat absorber and 6 ph = concentration of 150 mg l phenol time 4ph = 30 minutes with aniline concentration of 50 mg per liter in the contact time 45 minutes.

DDX25 Gene is located on chromosome 11q24 ,consists of 14 exons and effective for testicular function and spermatogenesis.DDX25 gene allele frequency in each population may be different.This difference may be due to environmental pressures and geographic areas are different.The impact of gene plays in the process of gametogenesis and Since Iran less attention to this issue and it has been scientific fields, this study was investigated the prevalence of genotype DDX25 in seven people, Persians, Turks, Baluchis, Gilaki, Arabs, Afghans.

In this study, DNA samples was extracted from 180 menby Salting out.Then, using Ase-I restriction enzyme to RFLP and PCR product was cut and the resulting components have been studied using electrophoresis.

In calculating the frequency of genotypes, 22/77% of the population have the genotype GG, 11/6% of genotype TT and 66/16% of patients had genotype GT. G allele frequency of 0.855 and 0.145 for allele T were equal.

According to the calculations performed by Chi-square test and Pvalue 0.48 In the study population, the ethnic Persians, Kurds, Turks, Baluchis, Gilaki, there was no significant difference between genotypes and blaring Arab population was not in Hardy-Weinberg equilibrium.

Uropathogenic Escherichia coli (UPEC) and Avian pathogenic Escherichia coli (APEC) is produce a wide range of human and animal virulence factors. These factors create colonization, invasion and the subsequent decline in the host immune response. These factors are include pap, sfa, afa, hly and cnf genes. The aim of this study was to molecular identification of adhesion, necrosis and hemolysin genes in UPEC an APEC isolated from poultry and humans samples.

A total of 36 isolates from clinical samples from patients with urinary tract infections in hospitals in Kerman and 36 poultry isolates were obtained from the veterinary faculty of Kerman province. Isolates were identified by standard biochemical tests. In order to identify, in vitro amlification Multiplex-PCR were performed for pap, sfa, afa, hly and cnf genes.

Genes analysis showed that in the poultry samples, 10 (27.7%) of the isolates contained acute genes and 22 (61.1%) of the isolates were in the human specimens in terms of presence of the gene. Cfa gene and cnf were not detected poultry and humans in any of the samples. The highest frequency of pap gene was reported in 33.33% in poultry samples and 13.8% in human samples.

The PCR method is a way in early detection of virulence factors in Escherichia coli isolates from animal and human but Epidemiological studies and rapid struggle with the disease can be used to determine the distribution and source of contamination.

Biosurfactant is a material valuable in industries such as oil, pharmaceutical, cosmetic, health food, medicine and agriculture industries. The aim of this study was to identify native biosurfactant-producing bacteria in oil contaminated soils. Sampling of petroleum contaminated soils around the city of Borujerd was done. After dilution and the cultivation, a variety of bacteria isolated. By using multiple tests of specifying biosurfactant-producing bacteria such as hemolysis test in blood agar, emulsification index measurement, destruction of drops, expansion of crude oil, the amount of petroleum hydrocarbons have been parsed biosurfactant-producing bacteria were isolated from other bacteria. The strongest biosurfactant-producing bacterial species were selected according to the screening tests and then was inoculated in Bushnell-Haas Broth medium with gasoline in a shaking incubator. After biosurfactant production and purification of the colony, to identify the genus and species of bacteria, a series of biochemical tests along with PCR of district 16 srRNA and sequencing were performed. With multiple experiments it was found the bacteria produce a significant amount of biosurfactant. After DNA sequencing, in the software review was found that more than 97% genetic similarities is between the bacteria and Bacillus subtilis. Due to the abundance of the bacteria in soil and its ability to produce biosurfactant and extent of oil pollution in Iran, the bacteria can be easily used to eliminate environmental pollution.

Spermatogonial stem cells (SSCs) are a self-renewing population of adult stem cells. These cells could restors reproduction throughout the life of the male by sperm production. In non-primate mammals, spermatogonial stem cells named A-single (As), and the A-paired (Apr) and A-aligned (Aal) are the progenitor spermatogonial population. In human, very little findings is available about SSCs. there are two different types of A spermatogonia, the Adark and Apale spermatogonia. The Adark spermatogonia were referred to as the reserve stem cells, whereas the Apale were considered the renewing stem cells. Regulation of the SSC population includes establishment and maintenance of a niche microenvironment in the seminiferous tubules of the testis. Signaling from somatic cells within the niche determines the fate of SSCs by either supporting self-renewal or initiating differentiation to spermatozoa. Although numerous expression markers have been helpful in isolating and enriching spermatogonial stem cells, no specific marker for this cell type has yet been identified. Culture of testicular cells on various feeder cells has made it possible to culture and expansion of spermatogonial stem cells for a long period of time and hormones and growth factors are investigated for their role in the process of proliferation of spermatogonial stem cells.