فهرست مطالب

Genetic Resources - Volume:3 Issue: 2, 2017
  • Volume:3 Issue: 2, 2017
  • تاریخ انتشار: 1396/06/10
  • تعداد عناوین: 7
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  • Ezatollah Esfandiari, Majid Abdoli * Pages 68-79

    Successful production and development of stable and adaptable genotypes only depend on the positive results achieved from the interaction between genotype and environment that consequently has a significant impact on breeding strategies. In this regard, we conducted an experiment to study genotypic differences of 16 lines durum wheat under both zinc sufficient and deficient stress during 2014-2015 growing seasons in University of Maragheh, Iran. Our results showed that Zn stress significantly (P < 0.001) affected all studied traits among the lines. The interaction between zinc stress conditions (C) and lines (L) was significant for peduncle length and plant height. Our findings indicated that zinc-deficient stress significantly reduced spike length (6.8%), spike dry weights (19.1%), plant height (12.0%), peduncle length (15.2%) and peduncle dry weights (26.7%). Zinc deficient stress also decreased the number of grains per spike, number of fertile spikelet per spike, thousand grain weight, biological yield, grain yield, and harvest index by 29.2, 15.5, 5.1, 24.1, 32.5, and 10.5%, respectively. The results showed that line numbers of 2 (G2, 4025) and 5 (G5, 46202) produced the lowest and highest spike length (SL) and spike weight (SW), number of grains per spike (NGS), and number of fertile spikelet (NFT), respectively; while line numbers of 10 (G10, 45704) and 14 (G14, 45415) produced the highest and line numbers of 1 (G1, 4017), 11 (G11, 45667), and 12 (G12, 45632) produced the lowest grain yield (GY), and harvest index (HI), respectively. Under non-zinc deficient stress and zinc deficient stress, GY was positively associated (P < 0.001) with STI, GMP, MP, and HARM as well as negatively correlated (P < 0.001) with SSI under zinc-deficient stress. Accordingly, indices of STI, GMP, MP, and HARM were the best indices for identification of high yielding lines in both conditions (zinc deficient tolerant lines). In total, results showed that G14 (45415) and G10 (45704) lines relatively identified as zinc tolerant and G1 (4017), G2 (4025), and G11 (45667) lines identified as susceptible lines.

    Keywords: Genetic diversity, Durum wheat, Agro-morphological traits, Zinc deficient resistance indices
  • Firooz Jannat Alipoor, Malek Hossein Asadi *, Masoud Torkzadeh Mahani Pages 80-86

    There are a sub-population of cells in tumor tissues known as cancer stem cells (CSCs) which have similar features with stem cells, including self-renewal and differentiation capacity. Recently, it was established that not only stem cells factors such as Oct4, but also ES-associated lncRNAs are contributing to various regulatory aspects of CSCs. Myocardial infarction associated transcript (MIAT) is a lncRNA which expressed in embryonic stem cells (ESCs) and its expression was regulated by ES-associated transcription factors, Oct4 and Nanog. Here, our results revealed that suppression of MIAT reduces cell proliferation and causes G1 arrest in HeLa cancer cells through downregulation of cell cycle progression factors including, CyclinD1 and PCNA. Furthermore, our data showed that downregulation of MIAT represses migration of HeLa cells which implicated that MIAT may be contributed to the migration of HeLa cells. We further detected MIAT silencing promotes apoptosis of Hela cancer cells and influences the expression of known apoptotic factors, Bcl2 and Bax. Based on our findings, LncRNA MIAT might be involved in the cervical cancer malignant progression and it could be the candidate as a potential therapeutic target to combat cancer.

    Keywords: LncRNA MIAT, Cervical cancer, G1 arrest, Apoptosis
  • Athar Javanmard, Maryam M. Matin, Hamid Rouhani, Mansour Mashreghi, Ahmad Reza Bahrami * Pages 87-97

    Cellulose is the most plentiful renewable biopolymer in nature which could be utilized by cellulolytic enzymes. Cellulases are among the most important groups of industrial enzymes which are widely consumed in biofuel production, pulp and paper, textile, and detergent industries. These enzymes can support a cleaner environment through reducing chemical processes in mentioned industries and agro-industrial waste management. Thermophilic filamentous fungi produce thermostable types of the enzymes with the property of hydrolysis the cellulose in higher temperatures with higher rates of reaction, decreased amounts of enzyme quantities and reduced risk of contamination by the mesophilic microorganisms. The cellulolytic capacity of two Thermoascus aurantiacus isolates (from Mashhad, Iran) was examined in a simple liquid state fermentation in different carbon and nitrogen sources, in comparison to the Thermoascus aurantiacus DSM 1831 as a reference fungus. Among different the tested sources, wheat bran and peptone led to the highest level of endoglucanase production by the isolated thermophilic fungi. The isolates showed higher cellulase activities, including endoglucanase, avicelase, and FPase, of the crude enzymes from the isolates in comparison to the reference fungus. Gene expression profiling revealed that changes in the cellulase mRNA levels are not correlated with the changes in protein activities during a 12-day period. This observation might be due to a complex process of enzymatic regulation of cellulases in response to the environmental signals.

    Keywords: Cellulolytic potential, Endoglucanase, Exoglucanase, β-glucosidase, Thermoascus aurantiacus, Thermophilic fungus
  • Mohammad Shokrzadeh, Abbas Mohammadpour, Nahid Masoudi, Mohammad Mostafa Nasirian Shakib, Seyedeh Marziyeh Mohammadi *, Yahya Saleh Tabari, Maryam Alizadeh Pages 98-102

    Gastric cancer is the fourth most common cause of malignancy death in the world, which also has a high prevalence in Iran. Caspase 3 gene is considered one of the major genes related to gastric cancer. The present study aimed to investigate the relationship between rs12108497 polymorphism of Caspase 3 gene. Due to the fundamental role of caspases, we have considered an SNP of Caspase 3 (common to two-way joint execution caspases), which has been proven to be related to various cancers. In this case-control study, 95 patients and 90 healthy subjects were enrolled. The patient status was approved, by endoscopy and pathology, to be gastroduodenal adenocarcinoma. Overall, 5 ml of the peripheral blood was collected from all participants, after obtaining an informed consent. Genomic DNA was extracted using the salting-out method and genotypic polymorphism was investigated using the PCR-RFLP method. The frequency of rs12108497 polymorphism was significantly different between patients with gastric adenocarcinoma and healthy subjects (p = 0.0259). Also, the difference in the distribution of T/T genotype was significant (P = 0.0111) and according to the OR score ((95.1% CI = 1.3539-10.5412) and OR = 3.7778), it is suggested that the T/T genotype increases the risk of disease. Our results confirm the association between the rs12108497 polymorphism of Caspase3 gene and the increased risk of gastric adenocarcinoma in individuals referring to Toba Sari Clinic.

    Keywords: Adenocarcinoma, rs12108497 polymorphism, Caspase3 gene, PCR-RFLP
  • Samaneh Pezeshk, Seyed Mahdi Ojagh *, Masoud Rezaei, Bahareh Shabanpour Pages 103-112

    In this study, hydrolyzed protein as a natural preservative for increasing the shelf life of minced meat of silver carp (Hypophthalmichthys molitrix) was used in a refrigerator for 12 days. Hydrolyzed protein was added to the minced meat of fish, at 0.5, 1 and 1.5% concentration. In this regard, microbial, chemical and sensory tests were carried out during the period of storage at intervals of 3 days. The results indicated that samples treated with a hydrolyzed protein of 1% and 1.5% were the best-tested groups to postpone the chemical and microbial degradation indicators and as well as the results of their sensory analysis were more acceptable. In general, the present study showed that hydrolyzed protein by a protamex enzyme from visceral of yellowfin tuna can be used as a natural preservative to increase the shelf life of the minced meat of fish.

    Keywords: FPH, Antioxidant, Antibacterial, Shelf life, Silver carp
  • Fatemeh Hasanzadeh, Navaz Kharazian *, Mohammad Reza Parishani Pages 113-129

    Saldaran protected region with about 14,000 hectares is located at longitude 50º34ʹE and latitude 32º4ʹN at 115 km of southwest of Shahr-e Kord City. This area is one of the important genetic resources in the Zagros Mountains. In the present study, floristic elements, life forms, chorological analysis, protected status, pasture and poisonous plants were investigated. All specimens were collected during all vegetation seasons and in several stages from March 2015 to July 2017. A total of 267 species, 190 genera, and 56 families were identified. The results show that 11 families, 41 genera, 51 species were monocotyledon, 44 families, 148 genera, and 215 species were dicotyledon and 1 species belonged to Pteridophyta. Based on life form data, the species were hemicryptophyte (41%), therophyte (35%), geophyte (16%), phanerophyte (5%), chamaephyte (2%) and parasite (1%). In term of geographical distribution, Irano-Turanian chorotype was a major component (57%, 152 species) in this region. A total of 153 mono-regional, 61 bi-regional and 53 pluri-regional species were identified. In addition, 32 endemic, 106 pasture and 23 poisonous species were determined. In terms of protective status, the species were near threatened (47%) and endangered (3%). The data obtained from the present study is reported for the first time for Iran.

    Keywords: Chorotype, Endemic, Irano-Turanian, Pasture, Endangered
  • Samaneh Kamalipour, Ali Barzegar *, Mohammad Shokrzadeh, Novin Nikbakhsh Pages 130-136

    Cytochrome P450 2E1 (CYP2E1) enzyme metabolically activates a large number of low molecular mass xenobiotics probably involved in gastric cancer incidence through activation of procarcinogens. North of Iran is amongst high incidence rate areas of gastric carcinoma where environmental carcinogenic compounds, including agricultural pesticides, are massively used. In this report, we quantitatively compared CYP2E1 gene expression between tumoral and nontumoral-marginal tissues of gastric cancer patients as well as normal healthy gastric tissues by real-time PCR. Results showed that CYP2E1 gene cDNA copy numbers were relatively increased in tumoral group vs. nontumoral-marginal and normal healthy groups. Comparison of means DDCT by Dunkan’s test statistically verified significant differences of cyp2E1 cDNA copy numbers between tumoral and healthy tissues (P=0.0018). It seems that the increased CYP2E1 gene expression may be associated with increased risk for gastric cancer. So, we recommended that CYP2E1 gene expression may be an appropriate molecular marker to determine individual sensitivity to gastric cancer and also for designing cancer prevention programs.

    Keywords: CYP2E1, Gastric cancer, Mazandaran province, Pesticide, Real-Time PCR