مجله سلول و بافت
سال دهم شماره 1 (بهار 1398)

 The aim of this study was to compare the gene expression changes and their effects on protein networks, transcription factors prediction, cellular pathways and small RNAs in the transgenic mouse models of Alzheimer’s disease (Tau and Amyloid beta).

 The results of the brain tissue microarray data from two models of Alzheimer’s disease were analyzed in GEO database. Protein networks prediction performed using String database and analyzed by Cytoscape software. The changed genes were used for prediction of transcription factors (TFs) and cellular pathways via Enrichr web service. Moreover, the ToppGene portal was used for prediction of the role of small RNAs involved in these models.

Analysis of protein networks have showed that the CTSS gene (encode Cathepsin S), a lysosomal cysteine protease, was the key gene and the main inter-network linker in the both models. Also, the data from evaluation of TFs resulting to introduce of IRF8 genes (encode Interferon Regulatory Factor 8) and NFE2L2 (encode Nuclear Factor, Erythroid 2 Like 2) that are involved in the immune system and oxidative stress respectively. On the other hand, we have shown that let-7 small RNA, which is involved in the immune system, could act as a major regulator in these gene pathways.

 The immune system has a critical role in the both models of Alzheimer’s disease and seems that the control of the immune-related genes (IRF8 and let-7) activity besides decrease of oxidative stress (CTSS and NFE2L2) in both models is a plausible therapeutic target.

 In this study, relationship between breast cancer and 251A/T polymorphism of IL-8 gene, as the genetic marker, was investigated in the Iranian females population by Tetra arms-PCR.

 In this case-control study, 50 women with breast cancer and 50 healthy women as control group were selected. After extraction of DNA, determination of IL-8 gene 251 A / T polymorphism genotypes was performed by Tetra arms-PCR.

 In the control group, frequency of AA, AT, and TT genotypes were 0%, 88% and 12%, respectively and them for case group were 12%, 54% and 34%, respectively. Result showed no significant difference between AA, AT and TT genotypes in the control and case groups (P ≤ 0.05).

 In current research, there was no significant difference between A and T alleles in the control and case groups. Therefore, it indicated that there was not association between IL-8 gene 251 A/T polymorphism and breast cancer in the studied Iranian females population.

The purpose of this study is to investigate the pro-apoptotic and anti-metastatic potentials of Lippia citriodora</em> alcoholic leaf extract on A2780 ovarian cancer cells and to evaluate the expression of E-cadherin as one of the most important marker in metastasis.

A2780 ovarian cancer cell line was prepared from Pasteur cell bank and cultured in RPMI1640 medium containing 10% FBS and 1% antibiotic. After cell seeding and treatment with different concentrations of extract (10-400 µg/ml), the cells viability and pro-apoptotic potential were examined by MTT assay and acridine orange/ propodium iodide, respectively. Caspase-3 assay and anti-invasive effect were analyzed by migration assay and assessment of E-cadherin expression, respectively. Then, one way ANOVA test was employed for analysis of quantitative data.

Data indicated that L. citriodora</em> alcoholic extract attenuated ovarian cancer cell viability. Acridine orange/ propodium iodide and caspase-3 assays showed that this extract in IC50 concentration (100 µg/ml) induced apoptosis mainly through caspase dependent pathway in the ovarian cancer cells. Migration assay and RT-PCR exhibited that this extract has anti-invasive capability and by up- regulation of E-cadherin prevents the loss of attachment between cancer cells.

The results revealed that L. citriodora</em> alcoholic extract has apoptosis inducing capacity and ability of restoration E-cadherin expression in A2780 cancer cells, which it able to suppress invasive potential of ovarian cancer cells.

This study was aimed to investigate the effects of Rosa canina (RC) fruit hydroalcoholic extract on lipid profile and liver anzymes level in diabetic mice.

 Ninty-six male mice were divided into 4 groups. The normal and diabetic groups received normal saline (p.o., 0.5 ml), however RC and RC-treated diabetic groups received RC hydroalcoholic extract (p.o., 500 mg/kg). Diabetes was induced by a single dose of streptozotocin (STZ) (i.p., 200 mg/kg) and 10, 20, and 30 dayes after diabetes induction the lipd profile (cholesterol, triglyceride, LDL, HDL and VLDL) and liver anzymes (ALP, ALT and AST) were evaluated.

Glucose level in the diabetic groups significantly(p<0.01) increased compared with the control group. Administration of the extract in the diabetic group resulted in significant reduction(p<0.01) in LDL, VLDL, triglyceride and cholesterol levels in comparison with diabetic animals. Administration of the extract in the diabetic group significantly increased (p<0.05) HDL level and decreased (p<0.01) ALT and AST levels.

RC fruit extract has the regulatory role in controlling lipid profile and liver anzymes level in mice models of diabetes.  

In the current study, effect of various concentrations of tamoxifen on CTNNBIP1 expression gene in the cancer cells derived from MKN-45 cell line of gastric cancer was considered as a therapeutic potential.

 The CSCs derived from the MKN-45 cell line were treated with different concentrations of tamoxifen for 48 hours and then cell survival was evaluated by the Trypan Blue test. After obtaining IC50, we examined the effect of 100 µM tamoxifen on the expression of CTNNBIP1 in CSCs as a therapeutic potential.

 Analysis of Real-time RT-PCR data showed that 100 μM tamoxifen in a 48-houred treatment can increase the expression of CTNNBIP1 gene.

 According to the results, it was concluded that tamoxifen has an inhibitory effect on the Wnt signal pathway and beta-catenin protein by increasing the expression of the CTNNBIP1 gene.

The aim of this study was to investigate the effect of Bisphenoln A on osteogenic differentiation in bone marrow mesenchymal stem cells obtained from adult rat.

Rat bone marrow mesenchymal stem cells were extracted using the flashing-out method. At the end of the third passage, cells were divided into groups of control and Bisphenol A treated with different doses of 1, 5, 10, 50, 100, 250, 500, 1000, 2000 and 4000 nM for period of 21 days in the osteogenic media containing 10% of fetal bovine serum. Then rate of Bone matrix mineralization, extracellular calcium deposition, expression of osteopontin and osteocalcin proteins was investigated during the procedure of osteogenesis. Data was analyzed using one-way ANOVA and the means difference was considered significant at p<0.05.

A significant reduction in mean bone matrix mineralization, calcium deposition, expression and synthesis of osteopontin and osteocalcin was seen in the group of cells treated with Bisphenol A when compared to control group (p<0.05) in a dose and time dependent manner.

These results showed that Bisphenol A ,as an environmental pollutant, cased a significant reduction in osteogenic differentiation in rat bone marrow mesenchymal stem cells; therefore, Bisphenol A can be considered as a reducing agent in cell differentiation.