فهرست مطالب

Reproduction & Infertility - Volume:21 Issue: 1, 2020
  • Volume:21 Issue: 1, 2020
  • تاریخ انتشار: 1398/11/02
  • تعداد عناوین: 9
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  • Mohammad Reza Sadeghi * Pages 1-2
  • Esmat Mangoli, Mohammad Ali Khalili* Pages 3-10

    Since the introduction of intracytoplasmic sperm injection (ICSI), the importance of sperm morphology assessment has been given attention in the assisted reproduction field. It is important to select a good-quality motile spermatozoon for giving a better embryo quality in assisted reproduction technique (ART). In ICSI, sperm morphology evaluation is limited due to its low magnification. However, by using intracytoplasmic morphologically selected sperm injection (IMSI), the selection is done at high magnification of ×6600 using motile sperm organelle morphology examination (MSOME). Therefore, it becomes possible to select a good quality spermatozoon with an intact nucleus that may enhance the pregnancy outcomes. Although all patients can benefit from IMSI, it is important to standardize which techniques (IMSI or ICSI) could be used or which group of patients benefit from IMSI to maximize the efficiency of this advanced technology.

    Keywords: ICSI, IMSI, Male infertility, Sperm morphology
  • Fatemeh Taheri, Marjan Omidi, Mohammad Ali Khalili, Azam Agha, Rahimi*, Mojdeh Sabour, Azita Faramarzi, Esmat Mangoli Pages 11-16
    Background

    The aim of this study was to assess the impact of total serum E2 on the day of human chronic gonadotropin (hCG) administration and the serum E2 per oocyte ratio on the outcomes of assisted reproductive technology (ART) cycles.

    Methods

    A total of 205 women were categorized into 3 groups according to the serum E2 levels: 1: ≤1500 pg/ml; 2: 1500-3000 pg/ml; 3: >3000 pg/ml. Another categorization included 3 groups according to E2/oocyte ratio: A: ≤150 pg/ml per oocyte; B: 150-200 pg/ml per oocyte; and C: >200 pg/ml per oocyte. The outcome compared between groups included laboratory and clinical characteristics. One-way analysis of variance (ANOVA), chi-square and Kruskal-Wallis, and multiple logistic regression model were performed, and appropriate differences were considered significant at p<0.05.

    Results

    There was a significant difference between the groups based on the E2 levels with respect to laboratory parameters. In group C, the rates of chemical pregnancy (54.1%), clinical pregnancy (50%) and live birth (45.8%) were significantly higher, when compared to other groups. Moreover, according to E2/oocyte ratio, the rate of live birth was higher in group C compared with group A (18.3%, p=0.04), and group C (29.7%, p<0.0001). Logistic regression showed the number of good quality embryos was a positive predictor for live birth (odds ratio=2.03, 95% CI=1-4.1), but the level of E2 on day of HCG was a negative predictor (odds ratio=0.99, 95% CI=0.99-1).

    Conclusion

    Supraphysiological levels of E2 had no adverse effects on the quality of the embryos in IVF cycles, but may have adverse effect on live birth in fresh transfer. Also, it is confirmed that both the pregnancy and live birth rates were elevated with E2/oocyte ratio ≥200 pg/ml.

    Keywords: E2, oocyte ratio, Estradiol level, IVF, Live birth rate
  • Diana Cárdenas Nieto, Maribel Forero Castro, Harold Moreno Ortiz, Elkin Lucena Quevedo, Juliana Cuzzi, Clara Esteban Pérez* Pages 17-33
    Background

    Assisted reproduction techniques (ARTs) and the preimplantation genetic test for aneuploidies (PGT-A) help couples with fertility problems to achieve a healthy live birth around the world. The aim of this study was to determine the rate of whole chromosomal copy number variations in embryos from couples undergoing ART and PGT-A, associations of chromosomal variations with embryo morphological parameters, and their relationship to maternal age.

    Methods

    This study included a retrospective analysis of the number of whole chromosomal copies identified by aCGH in embryos from couples undergoing ART.

    Results

    Seventy-six embryos from 29 couples using their own gametes were analyzed, of which 25 (32.9%) were chromosomally normal, and 51 (67.1%) were abnormal. Eleven embryos were evaluated from the group of couples with donated gametes, of which 5 (45.4%) embryos were chromosomally normal, and 6 (54.5%) embryos were abnormal. The main aneuploidies observed were trisomy X (7.8%), trisomy 21 (5.9%), trisomy 9 (3.9%), monosomy 11 (3.9%), monosomy 13 (3.9%) and monosomy X (3.9%), and the principal chromosomes affected were 19, X and 13. A significant association was found between the quality of the embryo and the genetic condition: embryos with euploidy and aneuploidy (p=0.046).

    Conclusion

    The rate of aneuploidies from couples with their own gametes was 67.1% (51/76) and from couples with donated eggs and/or sperm was 54.5% (6/11). The quality of the embryo determinated by the morphological parameters was not associated with the embryo genetic status, and also there was no association between maternal age and aneuploidy rate.

    Keywords: aCGH, Assisted reproduction techniques (ARTs), Copy number variations, Preimplantation genetic test for aneuploidies (PGT-A
  • Rachel Talia Bond, Alexandra Nachef, Catherine Adam, Marielle Couturier, Isaac Jacques Kadoch, Louise Lapensée, Gilles Bleau, Ariane Godbout* Pages 34-41
    Background

    The metabolic global approach is a multidisciplinary intervention for obese women before undergoing assisted reproductive techniques, with the goal of improving fertility and decreasing adverse pregnancy outcomes. The objective of this study was to evaluate the impact of the metabolic global approach on pregnancy rate.

    Methods

    This retrospective cohort study included 127 women and was conducted at the Centre hospitalier de l’Université de Montréal fertility center. Eligibility included BMI at initial consultation of ≥30 kg/m2. Fertility treatments were considered when a weight loss of minimum 5% and normal metabolic indices were achieved. The p<0.05 was considered statistically significant.

    Results

    Median baseline and last clinical assessment BMIs were 38.2 kg/m² and 35.8 kg/m² respectively (p<0.001), representing a median weight loss of 5.1%. At baseline, at least one metabolic parameter was abnormal in 66% of women. Total pregnancy rate was 53%. The majority of women (63%) who achieved pregnancy did so with weight loss and metabolic stabilization alone (11%) or combined with metformin (36%) and/or oral ovulation drugs (16%). Normal vitamin D (p<0.001) and triglyceride levels (p<0.05) as well as lower BMI after weight loss (p<0.05) were associated with an increased relative risk of pregnancy.

    Conclusion

    Replete vitamin D status, weight loss of 5% and lower BMI as well as normal triglyceride level are significant and independent predictors of pregnancy in obese women presenting to our fertility center. The metabolic global approach is an effective program to detect metabolic abnormalities and improve obese women’s pregnancy rate.

    Keywords: Assisted reproductive techniques, Infertility, Metabolic syndrome, Obesity, Vitamin D deficiency
  • Raha Khaneshi, Sousan Rasooli, Farnaz Moslemi, Sanam Fakour* Pages 42-48
    Background

    To diminish labor pain, several techniques have been used in developed countries. In the current randomized controlled trial, the use of epidural analgesia via PCEA pump with and without background infusion of analgesic was studied.

    Methods

    In this double-blinded controlled trial, 60 women were enrolled and randomly assigned to study groups for receiving epidural analgesia during labor. All patients received initial bullous dose including 125 mg bupivacaine and 3 mg/ml fentanyl, and the first group patient (CI) received background infusion of 8 ml/hr and the second group (PCEA) received 10 ml bullous dose of 125 mg bupivacaine combined with 100 mcg fentanyl (2 ml) via epidural catheter. The Visual Analogue Scale (VAS) of 0-10 was measured 20 min after drug injection. The chi-square and student T-test were used for comparing variables between groups, and 0.05 was considered as the level of significance.

    Results

    There was no significant difference in terms of demographic variables. Mean duration of the second stage of labor was significantly lower in patients received continuous infusion (CI) (p<0.0001). However, the total administered fentanyl dose was significantly higher in patients who underwent PCEA (p<0.0001). Besides, the CI group had a significantly lower rate of patient-controlled injection compared to PCEA patients (p<0.0001). However, there was no significant difference between patients’ satisfaction and VAS in study groups.

    Conclusion

    Epidural analgesia using PCEA combined with continuous infusion did not provide higher analgesia or patients’ satisfaction compared to PCEA alone; however, it led to a decreased rate of drug injection and total administered dosage.

    Keywords: Bupivacaine, Epidural analgesia, Fentanyl, Labor pain
  • Shaghayegh Alamin *, Tallat Allahyari, Behzad Ghorbani, Ali Sadeghitabar, Mohammad Taghi Karami Pages 49-58
    Background

    The approach of considering the psychosocial consequences of infertility has become significant. Psychosocial outcomes of infertility are defined and shaped in the context of the particular social and cultural conditions. Childbearing, and raising a child are considered to be the core of "identity" in many collectivist cultures, and the status provided to individuals with children is accompanied with social acceptance and approval. In such societies, infertile people see their social identity seriously damaged. The purpose of this study was to comprehend the challenge of identity crisis of infertile people in Iran for helping to formulate support programs for policy makers.

    Methods

    This qualitative study was conducted in 2016-2018, through semi-structured interviews conducted with 40 infertile clients of Avicenna Fertility Center. Data were analyzed by Strauss and Corbin coding paradigm.

    Results

    The core of the phenomenon of psychosocial consequences of infertility was failure in identity building, which included the main categories of prevention from continuity and prevention of forming normative identity.

    Conclusion

    Challenge of identity crisis based on the experience of infertile people and the social construction of infertility in their minds affects not only internal, external, personal and structural factors, but it is also a kind of identity search in individuals. Regarding this fact, providing appropriate social services and training the skills needed to rebuild identity of infertile people and their social health will be very effective.

    Keywords: Blood connection, Counseling, Culture, Femininity, Identity, Infertility, Masculinity, Social context
  • Farzane Alifi, Hamid Reza Asgari * Pages 59-64
    Background

    Currently, scientists are looking for a solution to the problem of the couples who have a lack of germ cells by through cell therapy. It is found that human amniotic membrane mesenchymal stem cells (hAMSCs) could be a good candidate for solving this problem. In the present study, an attempt was made to show that hUMSCs can express the PGC markers in the presence of retinoic acid (RA).

    Methods

    Placenta was obtained from healthy mothers and amniotic stem cells were isolated by enzymatic method from amniotic membrane. The cells were treated by retinoic acid for 14 days. Mesenchymal properties of hAMSCs were assessed by flow-cytometry and expression of PGC markers was established by Q-PCR.

    Results

    Mesenchymal stem cell properties were confirmed by antibodies against mesenchymal stem cell markers (CD73, CD90, and CD105). After that, the expression of the C-kit, Oct4, SSEA4, VASA genes were determined as primordial germ cell markers using quantitative PCR. It was found that the use of retinoic acid led to the highest expression of C-kit, SSEA4, VASA genes and lower expression of Oct4.

    Conclusion

    Our study indicates that retinoic acid can be used as a suitable factor for induction of hAMSCs into primordial germ cells (PGCs) and hAMSCs have enough potential to do that.

    Keywords: Amnion, Cell differentiation, Germ cells, Mesenchymal stem cells, Retinoic acid
  • Subhadra Poornima*, Swarnalatha Daram, Rama Krishna, Qurratulain Hasan Pages 65-67
    Background

    Similar rare Robertsonian and balanced reciprocal translocation in both child and mother with a history of multiple miscarriages in the first trimester was the motive to write this case report. Cytogenetic analysis helps in genetic counselling of infertility, BOH and dysmorphology which in turn helps in pre implantation genetic testing. Although many case reports have already been published about Robertsonian and balanced translocations, this is the first case report in India which showed both types of translocation in the same patient, rob (13;14) and t (4;7). Interestingly, in the same patient, same translocations were also identified in the mother and father having no chromosomal abnormalities.

    Case Presentation

    Proband with dysmorphology was refered first for karyotyping and later parental karyotyping was performed.

    Conclusion

    Cytogenetic analysis plays an important role in the diagnosis and management of disease along with prenatal screening.

    Keywords: Counselling, Culturing, Reciprocal translocation, Robertsonian translocation