فهرست مطالب

Molecular and Clinical Microbiology - Volume:9 Issue:1, 2019
  • Volume:9 Issue:1, 2019
  • تاریخ انتشار: 1398/03/11
  • تعداد عناوین: 6
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  • Hernan Vargas *, Jenny Sanchez, Alejandra Obando, Viviana Barbosa, Sandra Gomez, Patricia Arce, Esperanza Teuzaba, Sonia Salamanca, Carlos Golijow Pages 1074-1081
    The incidence of anal squamous cell carcinoma has increased in the last few decades. Anal cancer has been associated with persistent infection with high-risk human papillomavirus (HR-HPV) types. However, only a few data reflect the status of anal HPV infection in women with cervical neoplastic and paraneoplastic lesions. The objective of the present study was to investigate the distribution of HPV genotypes and abnormal anal cytology in sexually active women with cervical disease from Bogota, Colombia.
    Methods
    We therefore performed anal cytology for type-specific HPV identification in 134 anal samples of sexually active women from Bogota, Colombia, presenting cervical disease; using a commercial molecular technique (Linear Array®; Roche, Molecular Systems, USA).
    Results
    Results of anal cytology were normal in 93.3% of samples, while the remaining 5.2% was classified as atypical squamous cells of undetermined significance (ASC-US), 0.75% high-grade anal intraepithelial neoplasia (LGAIN-II/III,) and 0.75% low-grade AIN ( (HGAIN-I). The analysis for viral infection in the 134 anal samples showed HPV in 61.5% of the studied population. In general, higher HPV infection values were seen in more compromised anal lesions. The most prevalent viral genotypes were HPV-16 (27.7%), -6 (25.30%), -58 (15.70%), -18 (9.60%) and -53 (9.60%). Multiple infections were more common than single ones, and HR-HPV genotypes were present in 69.80% of positive samples.
    Conclusion
    Updating data concerning the distribution of genotypes that infect the anal mucosa may contribute to the implementation of strategies focused on reducing the rate of anal cancer morbidity and mortality.
    Keywords: Anal Cancer, Human Papillomavirus, Prevalence HPV, HPV Epidemiology, Female
  • Mostafa Norizadeh Tazehkand *, Orkideh Hajipour Pages 1082-1089
    This study was undertaken to select and analyze epitopes of Corynebacterium diphtheriae toxin using online and offline computerized analysis methods, after that affinity of MHC epitopes to HLA-DRB1*0101 was analyzed by Hex-protein protein software. The B cell epitopes were predicted via Immune Epitope Database (IEDB) and MHC class II epitopes were predicted by Vaxign softwarer. A physicochemical analysis of candidate vaccine revealed that the designed vaccine has a molecular weight of 59.062 kD. The estimated half-life of candidate vaccine was found to be greater than 30 hours (mammalian reticulocytes, in vitro), greater than 20 hours (yeast, in vivo), and greater than 10 hours (Escherichia coli, in vivo). The instability index (II) is computed to be 31.31 (<40), the aliphatic index was found to be 65.80, and the vaccine was considered stable. The grand average of hydropathicity was -0.580, therefore, the vaccine is a hydrophilic protein and probably it interacts with molecules of water. The result of allertop, toxinpred showed that the vaccine is non-allergenic and non-tixic. According to the obtained data from protparam and pepcalc, our designed vaccine is soluble and no transmembrane helix was projected, hence no expression difficulties are anticipated in the development of the vaccine. The membrane helices value of vaccine was 33.64%. The result of protein protein docking analysis showed that maximum affinity of candidate vaccine to HLA-DRB1*0101 with the score of -636.85. The result of this study showed that the candidate vaccine can be stimulate HLA-DRB1*0101.
    Keywords: In silico, Vaccine, Corynebacterium diphtheriae, B Cell, T Cell
  • Ania Ahani Azari *, Neda Kouroshzadeh, Hamidreza Pordeli Pages 1090-1096
    A large amount of feather is produced each year in the poultry industries which is buried in landfill or burned in the power plant generator. Feathers are purified keratinous proteins that can be degraded by bacteria and fungi. The aim of this study was to isolate and identify keratinase producing bacteria from the feather waste dumping sites around Gorgan district, Golestan province, Iran. Soil samples were collected from six poultry farms and slaughterhouses. The keratinolytic bacteria were isolated and identified based on feather and keratin degradation in feather meal broth and keratin agar. Isolates that showed more keratinolytic activity were identified using morphological, biochemical, and molecular tests. Then, the effect of pH and temperature was examined on the growth of the isolates. Based on the results, keratinase-producing isolates belonged to the Bacillus genus, among the isolates, the most keratinolytic activity was related to Bacillus megaterium strain SR7 and it showed the best growth at pH 6.8 and 37°C in feather meal broth and keratin agar. The results of the present study were consistent with the results of many similar studies and found that Bacillus strains are important producers of keratinase enzyme and therefore promising organisms for the management of chicken feather wastes through efficient biodegradation.
    Keywords: isolation, feather, Keratin-degrading bacteria, Molecular identification, landfill
  • Zahra Naeemi, Hadi Koohsari *, Hamidreza Pordeli Pages 1097-1107
    Antibacterial activity against gastrointestinal bacteria, tolerance to acidic conditions and bile salts are the main indicators of probiotics. The aim of this study was isolation of lactic acid bacteria with probiotic potential of bovine colostrum. Colostrum samples were cultured on selective culture. Identification of lactic acid bacteria carried out by biochemical tests of fermentation of sugars, growth at 10 and 45°C and 6.5% salt. Antagonistic effect of culture supernatant of isolates against native isolates S. aureus, E. coli, P. aeruginosa, B. cereus and standard strains E. coli, P. aeruginosa, S. dysentriae, S. aureus, B. cereus and E. faecalis carried out by well method and tolerance of isolates to acidic conditions and bile salts were evaluated. L. plantarum was the most species of isolated lactic acid bacteria (36.32%). Culture supernatant of L. plantarum more than other isolates was showed antibacterial activity. None of the isolates were able to grow at pH 2, but L. plantarum, L. casei, L. acidophilus, S. thermophilus and E. faecalis were able to tolerate pH of 3. Also, L. plantarum, L. casei, E. faecalis and E. faecium were able to grow in presence of 0.3 and 1% bile salt. Colostrum as a traditional dairy food can be considered as a very suitable reservoir for isolation of lactic acid bacteria with probiotic potential. Due to significant antibacterial activity of L. plantarum and the ability to grow in acidic condition and various concentrations of bile salts, probiotic potential of this isolate was evaluated as desirable.
    Keywords: Probiotic, Lactic acid bacteria, Antibacterial activity, colostrum, Tolerance to acidic conditions, bile salts
  • Noor Ridha Kadhim, Khosrow Chehri * Pages 1108-1113
    Stored nuts in the markets are naturally infected by different fungal species such as Aspergillus. The present study was carried out to evaluate the occurrence of toxicogenic A. flavus strains on nuts in Iraq. A total of 112 nuts samples including hazelnuts, pistachio, peanut, and walnut with typical symptoms of dark and green discolored lesions on kernels were collected from various markets in Baghdad. Strains of Aspergillus spp. isolated from nuts seeds and their morphological characterization was based on MEA, PDA, and CYA. The identification of A. flavus isolates were confirmed molecularly using primers T1/T2. A total of 25 fungal isolates belonged to Aspergillus species that were identified as A. niger (10), A. flavus (10), and A. japonicus (5). In molecular analysis, sequences of partial β-tubulin gene were blasted in GenBank to confirm morphological identification of A. flavus isolates. Aflatoxins (AFs) contamination of ten infected samples with A. flavus was evaluated using ELISA method. Natural occurrence of AFs could be detected in all tested samples, ranging from 6.50–74.48 μg/kg. Our results completed the previously data about genetic potential of AFs production of A. flavus strains in Iraq and revealed in infected nuts also can be one of the great concern in Iraq.
    Keywords: Food Safety, Taxonomy, mycotoxins, β-tubulin gene, Elisa
  • Saeid Mahdavi Omran * Pages 1114-1121
    This clinical trial study was performed on 144 patients suspect to have otitis externa between 2017 and 2018 in the ENT clinic of Ayatollah Rouhani hospital in Babol. For the treatment on the same day, the patients were categorized into two groups randomly. In the first group, after suction clearance, ciprofloxacin powder and betamethasone ointment were used, while in the second group after suction clearance, ciprofloxacin drop and betamethasone drop were used in the ear canal. All patients reevaluated after one week. After preparation of the results of culture of the samples, 83 individuals including 44 in the first group (ciprofloxacin powder and betamethasone ointment) and 39 subjects in the second group (ciprofloxacin drop and betamethasone drop) had bacterial otitis externa. The mean intensity of pain in the first and second groups was 2.61±2.13 and 2.8.00, respectively which showed a significant difference (Pvalue<0.001). The itching was 38 (86.4%) and 6 (15.4%) cases in the first and second groups, respectively, which showed a significant difference (Pvalue<0.001). The discharge in the first and second groups was 10 (22.7%) and 1 (2.6%) respectively which again had a significant difference (Pvalue=0.007). The extent of diminished hearing in the first and second groups was 17 (38.6%) and 0 (0%), respectively, indicating a significant difference (Pvalue<0.001). Finally, inflammation in the first and second groups was 20 (45.5%) and 0 (0%), showing a significant difference (Pvalue<0.001). This study indicated use of ciprofloxacin powder and betamethasone ointment is less effective than applying ciprofloxacin drop and betamethasone drop.
    Keywords: antibiotic, bacterial infection, direct examination, mycology, culture