فصلنامه تازه های بیوتکنولوژی سلولی مولکولی
پیاپی 37 (زمستان 1398)

The HMGA2 protein is one of the transcription factors that regulates the evolution and differentiation of the cells. Studies have shown that changes in the expression of the HMGA2 gene can play an important role in preventing tumor progression and its metastasis. In this study, we investigated the association between rs10573247 polymorphism in the 3-UTR region of HMGA2 gene and the risk of gastric cancer.

Genomic DNA from blood sample of 100 patients with gastric cancer and 100 healthy individuals were extracted A part of the HMGA2 gene, including rs10573247 polymorphism, was amplified by PCR technique. After, the PCR product was treated with EaR1 enzyme and the genotype of each individual was determined. Finally, the risk of gastric cancer was assessed with the statistical tests of χ2 and logistic regression.

The statistical analysis of allelic and genotypic association of rs10573247 polymorphism with the risk of gastric cancer showed that there was not significant relationship between different alleles of rs10573247 polymorphism (P = 0.127) and none of its genotypes with gastric cancer risk.

It seems none of the rs10573247 polymorphism genotypes in the HMGA2 gene were associated with the risk of gastric cancer.

Endometriosis is one of the major causes of decreased fertility in mares which is caused by various infectious agents, the most common of which is Escherichia coli. The aim of this study was to determine the virulence factors pattern in Escherichia coli isolated from endometriosis cases in mares.

In this study, 136 mares with a history of infertility and pregnancy problems were studied in Chaharmahal and Bakhtiari province. After sampling by uterine siphoning and microbial culture of the samples, molecular confirmation of the isolated Escherichia coli strains and the presence of the most common virulence factors in these strains were used by PCR method.

From 136 studied samples, 22 (16.17%) were infected with Escherichia coli. It was recognized that fimH (90.9%), afa/draBC and cnf1 (72.7%) had the highest while papGIII (27.2%), and traT (18.8%) had the lowest distributions of virulence genes in these strains.

The results of this study showed that one of the dominant bacteria causing endometriosis in Escherichia coli material is the presence of a variety of virulence factors in Escherichia coli strains indicating direct involvement of these agents in bacterial pathogenicity.

Recently, studies have been reported in vitro and in vivo, that these pyridopyrimidine compounds have a beneficial effect on chronic diseases such as cancer. this study was designed to evaluate the effects of synthetic pyridopyrimidine compounds on the growth and proliferation of normal and cancer cell lines.

Cytotoxic effect of synthetic pyridopyrimidine at different concentrations and period on the MCF-7, MAD-MB-231, MOLT-4, PC-3, and Normal Fibroblast (HFF) cell lines were cultured in 96-well plates, thin cells were treated with synthetic compounds at different concentrations 1000, 500, 50, 5 nM at 24,48 and 72 hours, to evaluate the effect using MTT assay. the resulting data were analyzed using SPSS16 software with one-way ANOVA followed by the Tukey test and P <0.05 was considered as a significant difference.

The results of this study showed that pyridopyrimidine compounds had no significant toxicity effect on the normal cell lines, Nevertheless showed strong and dose-dependent inhibition of cancer cell growth at P-value <0.05. also, results showed that pyridopyrimidines with bromide groups at a concentration of 500 to 1000 nM had a more effective effect on inhibition of cancer cell growth inhibition at 72 hours.

Results of this study indicated the pyrimidines inhibition effect on cancer cell lines, however, the use of these compounds as an attractive option in chronic diseases treatment in the world as alternative medicines or combination with conventional medicines are suggested

The increase in kidney disease is partly due to an increase in the prevalence of diabetes. Type 2 diabetes often occurs in adults.. The aim of this study was to investigate the relationship between HbA1c   and microalbuminuria in type II diabetic patients in Isfahan province by biochemical methods.

In this study, 50 patients with type II diabetes and 50 healthy subjects were blood samples. Also, microalbumin was collected from both groups of 24-hour urine samples. Then, HbA1c   was measured by HPLC method and the microalbumin content of the specimen was measured by Immunoturbidimetric method. Data analysis was done using SPSS software. Comparison of quantitative data was performed using T-test, qualitative data with A, qualitative data with Chi-square test and U Mann Whiteny test.

The results indicate that with increasing levels of HbA1c  , the rate of microalbumin release also increases in type II diabetic patients. Biochemical indices such as FBS, BUN, Cr, U.Acid, potassium, serum albumin were significantly higher in the control group than in the control group.

According to the results obtained from this study, it can be concluded that type 2 diabetes mellitus causes an imbalance in the excretion of microalbumin from the kidney. Also, the study of HbA1c   is a useful parameter in the evaluation of type 2 diabetes.

Increasing population and urbanization, industrialization and inappropriate use of land have created numerous environmental challenges that contaminate water resources as one of its major implications. The discharges of wastewater and industrial wastewater at inappropriate places and the penetration of these effluent into the soil causes pollution of underground aquifers and subsequent pollution of well water. Underground water and rural water supply wells are more exposed to microbial and chemical contamination due to their remoteness from the urban service area. Therefore, in this research project, we decided to study and evaluate the amount of microbial and physicochemical pollutants of drinking water in Urmia wells.

This study was conducted within 6 months. Five sections, including the soomay baradost, the central part of Urmia, anzal, nazluo, and the Silvan section, were selected with the guidance of a respected expert from the West Azarbaijan Rural Water and Wastewater Company. The COD، BOD5, Total colifom (TC), Fecal coliform (FC) and Ecoli by The MPN method was measured and measured. Also, the probable chemical elements were measured based on Iran's standard organization protocols

The results of physicochemical experiments showed that the samples taken on the basis of 1053 Iran standard organization have a desirable level. Also, regarding the parameters of fecal coliform, total caloric and E. coli, which are water resources pollution index, the results of all samples were reported from wells in selected villages without any contamination.

The results of the experiments showed that the well water in the selected villages of Urmia has a good level of microbial and chemical parameters, which indicates the effectiveness of the methods and policies applied in the management and monitoring of water resources.

Diabetes is a metabolic disease that is caused by a chronic increase in blood sugar due to a defect in insulin secretion or function. This study demonstrates the relationship between serum zinc, copper, magnesium and chromium trace elements in the patient population.

In this study, subjects were randomly selected in three groups, including 50 females, 50 males and 100 controls. Data were collected through interviews and questionnaires. Serum trace elements in the blood of fasting patients were evaluated using atomic absorption spectrometry, direct calorimetry and photometry.

Evidence from this study indicates that the mean serum zinc and copper concentrations in diabetic compared to non-diabetic patients were significantly decreased (P <0.001) and (P = 0.025). On the other hand, serum concentrations of chromium and magnesium in diabetic and non-diabetic subjects were not significantly different. Linear regression analysis of gender was not statistically significant and the decrease in serum zinc and copper concentrations was independent of individual gender.

Evaluation of serum concentration of trace elements shows that the concentration of two elements of zinc and copper in diabetes is significant and can be an appropriate marker for the probability of diabetes. Decreased zinc levels and unchanged levels of chromium and magnesium in diabetic serum should be sought for other factors such as individual conditions, urinary incontinence and Interventional factors that require further study.

Narcissus family plants are important in addition to their ornamental value in terms of aromatic and medicinal compounds. The present study was conducted to investigate the quantitative and qualitative properties of phenolic compounds and antioxidant activity of four genotypes of Narcissus with the local name: Shahla, Meskin, Shasteper and Panjeh gorbeie in two phenological stages (beginning and end of harvest season).

For this purpose, after collecting plant materials in the natural habitats of Behbahan, drying and cold extraction were done. In this experimental study, the phytochemical study of the plant was first performed, then the total phenolic and flavonoid compounds were measured by spectrophotometric method. Finally, the antioxidant activity of the extract at different concentrations was determined using DPPH assay.

The results showed that the extract yield in the sample of Maskin was more than the other samples at the beginning of harvest season. Also, compounds such as tannin and alkaloids were observed in some samples, that, varied in different these genotypes. The high percentage of DPPH inhibition is also confirmed the high antioxidant properties of these genotypes, although the three genotypes had the same degree of DPPH inhibition level, but the highest antioxidant activity is related to Meshkin with inhibitory percent 72.93 and the least anti-oxidant property related to Shahla with an inhibition rate of 91.895. According to the results, Maskin and Shahla genotypes showed the highest and least antioxidant properties, respectively.

The results of this study indicate that there is a significant difference in the composition and percentage of inhibition of DPPH free radicals in the extract of various nursery plants, which has not been reported for this plant in scientific sources.

Many single nucleotide polymorphisms have been identified in the androgen receptor coding gene that play an important role in the development, prediction, and diagnosis of cancer. In this regard, this study was designed to evaluate the allelic frequency of rs137852579 single-nucleotide polymorphism in the androgen receptor coding gene in patients suffering from prostate cancer.

In this study, peripheral blood was taken in 10cc out of 50 prostate cancer patients and 50 healthy individuals. Genomic DNA of the samples was extracted and isolated. ARMS-PCR and direct sequencing were used to determine the presence of the desired polymorphism in population.

The results of this study showed that the allele frequency of rs137852579 for the C allele in the healthy group was 0.06 and in the patient group was 0.28. In addition, the heterozygosity rate showed that the Iranian population was in Hardy Weinberg equilibrium for this polymorphism (p> 0.05).

Based on the results of this study, it can be concluded that rs137852579 polymorphism in the androgen receptor gene can contribute to prostate cancer in the Iranian population.