مجله علمی پزشکی جندی شاپور
سال هجدهم شماره 6 (پیاپی 123، بهمن و اسفند 1398)

Euphorbia microsciadia Boiss is a plant used in traditional medicine for cancer treatment in many countries. The purpose of this study was to determine the effect of E. microsciadia extract on the elimination of tumor cells and the expression of cytokines in the breast cancer cell line MDA-MB-231.

The cells were cultured in DMEM medium supplemented with 10% fetal bovine serum, penicillin and streptomycin. The effect of the extract on the viability of MDA-MB-231 cells was evaluated by MTT assay. To evaluate the expression level of the mRNA after MDA-MB-231 cell line treatment with the plant extract, the RNA was extracted to cDNA synthesis. The mRNA expression level was measured in the samples by TaqMan real-time PCR technique.

After 24 and 48 h, the IC50 values for the E. microsciadia extract were 278.30 and 238.20μg/ml, respectively. The extract exhibited anti-proliferative effects on the MDA-MB-231 cell line in a dose- and time-dependent manner. The expression levels of INF-γ IL-8 and IL-1β were significantly increased 24 and 48 hours after the extract treatment. The expression levels of TGF-β1, IL-6, IL-10 and IL-17A were increased after 24 h of treatment and decreased after 48 h of treatment.

The E. microsciadia extract may be used as a tool to manipulate the expression of cytokine genes and ultimately control cell growth and proliferation, which is essential for molecular and cellular studies in the cancer therapy.

A review of effective treatments for adult stuttering Abstract Speech fluency is one of the characteristics of natural speech. Any disturbance in the Continuity, speed of production, planning, speech rhythm or a combination of these factors will cause a phenomenon known as nonfluency or stuttering. Aim of this study was determining the effective treatments for adult stuttering. In general, stuttering for adults focuses on either (1) teaching new ways of managing and dealing with stuttering, or (2) teaching new speech techniques that facilitate fluent speech. These two approaches (stuttering management and speech restructuring) are commonly used together to help the stutterer deal with core behaviors of stuttering as well as the affectional, anxiety, emotional, and cognitive aspects of the disorder. Pubmed, Google Scholar, and Scinece Direct were used to perform this study. Finally, we selected eighteen related articles based on purposes of this study. 

Low back pain is one of the most common and costly medical problems and the main cause of absenteeism. The aim of this study was to investigate the effect of conventional core stability and special suspension exercises on multifidus muscle endurance, pain and quality of life in non-specific chronic low back pain patients.

This is a quasi-experimental study. The statistical population of the study in patients with chronic low back pain referring to neurological and orthopedic clinics as well as the health center of Sama in Neyshabur city, the samples were selected purposefully. The severity of low back pain, multifidus muscle endurance and quality of life was measured using the test and Quebec questionnaire, Electromyography machine during McGill trunk endurance tests and SF-36 questionnaire. These measurements were repeated after 12 weeks of both exercise in both groups.

The results showed that both types of stability training reduced the amount of pain and increase the trunk muscle endurance and have improved the quality of life in terms of physical and mental aspects and physical function in patients with non-specific chronic low back pain. This decline was significant in special suspension exercises.

Core stability exercises can be used more effectively than core stability exercises on fixed surfaces for these patients, given their more favorable effects on patients.

Osteoarthritis is a common disease and painful arthritis with unknown etiology. In this study, the effects of mesenchymal stem cells and aerobic training on apoptosis biomarkers (BAX and BCL2) in the heart tissue of osteoarthritic rats was investigated.

In this experimental study, 42 male Wistar rats (age range 6-8 weeks and body weight of 250-300 g) were divided into two groups: osteoarthritic group (n=35) and healthy control group (n=7). osteoarthritis was induced in rats by surgery. then osteoarthritic rats were divided into five subgroups of seven including control, saline, stem cell , aerobic training, and aerobic training+stem cell groups. aerobic training program was conducted for 8 weeks and 5 days a week. apoptosis biomarkers, including BAX and BCL2 proteins in the heart tissue of rats, were measured using ELISA kits. One-way ANOVA used to analyze the data.

BAX concentration was significantly more in saline and unhealthy control groups compared to other groups and BCL2 concentration was significantly less in saline and unhealthy control groups compared to other groups (p<0.001). aerobic training and stem cell, whether used separately or together, caused a significant increase in BCL2 concentration and a significant decrease in BAX concentration (p<0.001).

Osteoarthritis caused an increase in heart cell apoptosis. Although stem sells have anti-apoptosis effects, they will be more effective in treatment if they are used with aerobic training.

Background and objects:

 Autism spectrum disorder is one of the disorders of growth, and so far, different methods have been developed to reduce its symptoms. In this research, the method of intensive interaction has been studied. The purpose of this study was to investigate the effectiveness of intensive interaction intervention on social interactions and communication of children with autism spectrum disorder.

This study is due to the use of a non-randomized, semi-experimental with control group and pretest, post-test. The population of this study included children with autism spectrum disorder that was selected by available sampling among the centers of autism in Ahvaz, Iran, and 11 children were randomly assigned to both experimental and control groups. The Gilliam Autism Scale (Gars) was used to measure the variables of the research. To analyze the results, descriptive statistics including mean and standard deviations of scores were used and the covariance analysis was used to control the interventional variables in the pretest.

According to the findings, the method of interactive interaction is effective on the social interaction dimension, but it is not effective on the communication. communication mainly involves the use of language and is more specific than interaction in general. Therefore, the effectiveness of this method was confirmed on social interaction but was not confirmed by social communication. 

Computer-aided design diagnostic systems are widely used in the differential diagnosis of breast cancer. Therefore, improving the accuracy of a CAD system has become an important field of research. In this paper, we investigated CAD systems based on deep neural networks of convolution type to detect breast cancer in thermographic images.

For analyzing the proposed model, the DMR database has been used. The number of the participants examined were 196, including 41 cases of cancer and 155 healthy subjects. Each person had 10 images of thermography. The total number of the analyzed images included 1960 images of thermography. The classification of thermal images including cancerous and healthy images is based on three types of deep convolution neural networks including google net, resnet18 and vgg16.

The accuracy and specificity of the results using a neural network models of deep pre-training on google-net, resnet18 and vgg16 is 85.03%-89.7%, 83.8% -91.9% and 85.03% -91,01% respectively. The proposed model is capable of providing a significant response to the different breast tissue morphologies.

The model of deep artificial neural network can be used as an efficient and intelligent way to detect cancer in original thermal images without extracting features. However, more studies are needed to design other models of artificial neural networks based on deep learning to detect malignant or benign cancer in thermal imagery.

Genital Herpes is one of the most common sexually transmitted disease in the world especially in women and has high prevalence in developed and developing countries. It has third Couse of sexually transmitted diseases after Gonoccocal and Chlamydia infections in the world. HSV-2 is the main couse of genital herpes . The aim of this study was assessment of the prevalence of HSV-2 in pap-smear samples were investigated from Ahvaz's married women whit PCR method.

In this cross-sectional study, 276 pap-smear samples from women aged 16-72 years old that refferd to women clinics in 2017 and 2018 years prepared and after DNA extraction, virus identification in samples was performed using specific primers and PCR test.

In this study, 24 samples (%8.7) from married women were contaminated by HSV-2. The highest frequency of infection was observed in the age group of 46-55 years old and in women with normal cytology and mild inflammation.

According to the gathered results it could be concluded that infection prevalence by Herpes virus is more in age group of women with high sexual activity. It could be said that with reference to relatively high infection preference by this virus in active sexually groups, frequency investigation of this virus at 5 years period and programming to increase awareness of general population about disease, its transfer and prevention methods, are necessary and essential to infection decrease in total population at massive level.

Introduction:

Galectin3 has been recognized as a new biomarker for cardiovascular disorders, However the effects of aerobic training on theses markers have not been identified. Therefore, the purpose of the present study was to investigate the effect of aerobic training on serum levels of Galectin3 and inflammatory markers in elderly women with metabolic syndrome. Subjects and

24 elderly women with metabolic syndrome (age: 65.37±4.08, weight: 88.98±6.56, BMI: 34.14±1.47) were randomly divided into two groups of aerobic training (n=12) and control group (n=12). The aerobic training protocol was administered for 12 weeks, three sessions per week with intensity of 65-50% of maximum heart rate. Serum samples were collected in pre-test and post-test for ELISA using Galectin3, TNF-α and CRP. Results. Aerobic training resulted in a significant decrease in serum Galectin3 compared to control group (P˂0.05). Also, aerobic training was associated with a significant decrease in inflammatory markers (CRP and TNF-α) (P˂0.05). 

Conclusion:

The findings of the present study showed that aerobic training is associated with a decrease in serum Galectin3 in elderly women with metabolic syndrome, which may be due to the improvement of chronic inflammation.

stuttering is a disorder in fluency of speech interrupted due to repetition, block, prolongation and hesitation. Frequency of this disorder is around 1% in population. Stuttering affected on a variety of aspect of speech, language, emotion and psychological of the person has stuttering. The aim of the present research was comparison of emotional intelligence in adolescents stutterers and non-stutterers in Isfahan. Subjects and

In this research we used of Bar-ON emotional intelligence questionnaire to investigation of effects of stuttering on emotional intelligence. To do this research, we estimate reliability of the questionnaire on 30 adolescents without stuttering and then used of it on 21 adolescents stutterers group and 30 non-stutterers group.

The results of the present research showed that the general scores of two groups had not significant difference. The persons with stuttering had more scores in flexibility but there was not any significant differences in this aspect.

There are not any significant differences between two groups and these might to stuttering had not any effects on emotional intelligence in adolescent. In addition, high score in flexibility in stutterers group might showed that fluency disorder can help them to adjust their thinks and behavior with environmental and conditions changes more than non-stutterer groups in this age.

The present study was designed to evaluate the frequency of natural endopeptidase (NEP) or CD10 in prostate carcinoma cells in order to evaluate its association with tumor prognostic findings.

In this cross-sectional descriptive study, tissue samples of patients referred to Imam Khomeini, Shahid Baqaei and Golestan hospitals of Ahwaz with the diagnosis of prostate adenocarcinoma and benign prostatic hyperplasia were used. Clinical and demographic data of patients were extracted from hospital records.. Anti CD10 monoclonal antibody was used for immunohistochemical staining.

In this study, 51 prostate samples (41 malignant and 10 benign) were studied. The mean age of the patients was 68.4 years, ranging from 40 to 92 years. Stage of cancer was organ confined in the majority of cases (75%). The pattern of expression, expression intensity and staining intensity in malignant specimens were significantly different than benign tissues. CD10 expression decreased in more than 50% of malignant cases. Also, the staining intensity of this marker was significantly lower in malignant samples. In addition, evaluation of correlation between staining intensity with other studied variables showed that there was a significant relationship between staining intensity and gleason score (p <0.0001).

Overall, the findings of the present study indicate that expression of the CD10 marker in malignant prostate tumors is decreased or abolished. However, this marker is expressed in advanced stages of the tumor and is related to the invasive pattern of the tumor.