Journal of Basic Research in Medical Sciences
Volume:4 Issue: 3, Summer 2017

Helicobacter pylori (H. pylori) infection has remained as a global health problem. Animal studies demonstrated the role of H. pylori oipA gene in the development of gastric cancer. The aim of this study was the cloning and expression of Helicobacter pylori oipA gene in a bicistronic vector harboring mice IL-18 gene.

The target gene encoding oipA was amplified from a codonoptimized clone by PCR, and then double-digested by restriction enzymes. The pIRESIgk/mIL18/Fc plasmid was simultaneously digested by BstXI/NotI enzymes to elicit the eGFP segment. PCR product of oipA was inserted into pIRES-Igk/mIL18/Fc plasmid using T4 ligase. Transformation into DH5α strain was done. Cloning was confirmed by PCR, enzymatic digestion and sequencing. Expression of the oipA and IL-18 mRNA was assessed by means of TaqMan Real-time PCR.

Electrophoresis of PCR product, enzymatic digestion and sequencing showed that the H. pylori oipA gene was successfully cloned into pIRES-Igk/mIL18/Fc to generate mIL18-pIRES2-oipA plasmid. The results of Real-time PCR confirmed the successful expression of both oipA and IL-18 in mouse macrophage cell line.

Considering the role of oipA in pathogenesis of H. pylori and potent activity of IL-18 as a molecular adjuvant, the results of the present study showed that the expression of codon-optimized oipA gene in bicistronic vector including mouse IL-18 is successful. So, it could be considered as an appropriate genetic vaccine candidate for H. pylori in future investigations.

One of the most important problems in student's education is academic failure. Identifying factors that affecting academic achievement and pay attention to these factors is a stride toward success. Students' academic achievement and related factors have priority in education research. This study aimed to determine the relationship between learning study strategies and academic achievement of nursing students in 2013.

This descriptive- analytic study was performed among 85 nursing students of Ilam University of Medical Sciences. By using the learning and study strategies inventory (LASSI), the main three components of study and learning strategies, including items of skill, will and self-regulation were examined. The Pearson correlation was used to measure students' academic achievement correlation with the mentioned above components.

The sample consisted of 50.6% female and 49.4% male. The mean grade of nursing students was scored 15.60. Pearson correlation coefficient showed that the three main components of learning strategies and study skills component, respectively skill (P=0.001, r=0.349), self-regulation (P=0.009, r=0.280) and the component of will (P=0.045, r=0.218) with student achievement (mean grade of last term) had a positive and significant relationship.

Considering positive and significant correlation between learning and study strategies with academic achievement in nursing students, so, we suggest that students and teachers use these strategies to improve academic achievement and reduce the academic loss of students.

Myocardial infarction (MI) is associated with increased oxidative stress and reduced antioxidants. Some studies have shown that exercise training increases the serum level antioxidants. Therefore, this study investigated the effect of rehabilitation training on antioxidant status in patients with myocardial infarction.

In this study, 20 patients with myocardial infarction were selected and randomly assigned to training group (n=10) or control group (n=10). Training program included 3 sessions of concurrent training per week for eight consecutive weeks. To measure the values of malondialdehyde (MDA), protein carbonylation (PC) and total antioxidant capacity (TAC), 10 ml of blood were taken pre- and post-training in each patient. The t-test was used to analyze the data. P<0.05 was considered significant in all tests.

The data showed that 8 weeks of rehabilitation training significantly reduces MDA (2.37±0.59 µM and 3.74±1.34 µM in training and control groups, respectively; P=0.000) and PC (9.15±1.77 nmol/mg protein and 11.48±1.60 nmol/mg protein in training and control groups, respectively; P=0.006) levels and significantly increases TAC (10.09±1.70 U/ml and 8.34±1.56 U/ml in training and control groups, respectively; P=0.031).

Since the findings of the present study show a reduction in oxidative stress markers (MDA, PC) and an increase in total antioxidants capacity, it seems that eight weeks of concurrent training may improve the antioxidant capacity in patients after myocardial infarction.

Spirituality, as with the knowledge of the whole universe, is a force beyond the material aspects of life. Self-esteem is generally a component of evaluation of selfconcept and the suicidal thoughts is a condition that implies on the occurrence of any selfdestructing thoughts.

Methodology of the present study was descriptive and correlational. The study population included all graduate students of Azad University of Ilam from which 235 persons (138 men and 97 women) were selected through stratified random sampling. The measuring tools of data were as follow: Kings spiritual understanding questionnaire, Eysenck self-esteem questionnaire and Beck suicidal ideation scale. The data was analyzed with using the statistical software SPSS the statistical methods, Pearson correlation coefficient, analysis of variance, multiple regression analysis and structural equations.

Spiritual understanding and self-esteem reversely and significantly predicted the thoughts of suicide with the coefficients -0.194 and -0.077, respectively.

Individual factors such as spiritual perception and self-esteem are important and effective on the mental aspects of students. By increasing the impact of these factors, it could be expected to reduce the number of suicides.

It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown.

To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thrombin-activated platelets. After the elapsed co-culture time, the levels of caspase-3 and CD95 were evaluated as a sign of cell death and apoptosis. In addition, immortalized cells were evaluated using trypan blue, 7-AAD and WST-1 methods.

CD95 and caspase-3 levels were significantly increased in both cell lines compared with the control cells (P<0.05). Beside, considerably lower number of living cells were shown by trypan blue, 7-AAD and WST-1 in the treatment groups compared with the control group (P<0.05).

This study demonstrated the role of apoptosis in tumor inhibition and implied the ability of platelets to induce apoptosis in tumor cell lines.

The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi.

We described a simple procedure for purifying human HLA molecules from the cells lysate. As a representative model, HLA-DR was purified from Daudi cell line. The cell membrane was solubilized by a buffer contained NP-40 detergent. Subsequently, the isolation of the membrane antigen was carried out by affinity chromatography method using mouse anti-human HLA-DR monoclonal antibody. The size and the specificity of the purified antigen were determined by Bradford and ELISA methods, respectively.

The purified HLA antigen was obtained in approximately 20-30 micrograms in each run of chromatography. Additionally, ELISA method demonstrated the HLA-DR specificity of the purified protein.

The results indicated that affinity purification of HLA-DR antigen by means of specific monoclonal antibody is a simple and fast procedure for obtaining the purified antigen.

Toxoplasma gondii (T. gondii) is distributed worldwide and infects most species. The serious incidence and severe or fatal injury caused by T. gondii infection clearly indicates the necessity for the event of a vaccine. The current study goals were to evaluate serological applications of Toxoplasma gondiirhoptry protein 1 (ROP1) antigen.

We created a polymer vaccine by using the eukaryotic plasmid, pROP1. Purification by one-step metal affinity chromatography allowed recovery of milligram amounts of purified recombinant proteins per liter of culture. The quality of this matter for diagnosing of human infections was provided and tested on 77 serum samples which were obtained during routine diagnostic tests. A panel of 20 serum samples from patients with acute toxoplasmosis was compared to a panel of 35 serum samples from individuals with chronic toxoplasmosis.

Results of the study indicated that antibodies detected from patients with acute and chronic infections were 96% and 17%, respectively, by using of pROP1 recombinant antigen.

According to the present study an immunoglobulin G antibody against ROP1 antigen is made throughout the acute stage of toxoplasmosis infection, but not in the chronic phase of toxoplasmosis.

The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples.

Thirty surface water samples were collected from Rasht City rivers and lagoons during 2009-2010. The samples were analysed for Cryptosporidium oocysts using Real Time PCR method. Samples were filtrated through a 1.2µm pore size cellulose nitrate membrane filter and then purified and quantified by Real-time PCR technique.

Cryptosporidium oocysts were found in 19 of 30 the samples. Oocyst concentration was ranging from 0.007 to 27 oocysts per liter of the examined waters.

The present study showed that the investigated water supplies were contaminated by Cryptosporidium oocyst. This study indicated that in this level of oocysts there is a potential risk of waterborne cryptosporidiosis due to direct or indirect consumption of these waters by humans and animals. Real-time PCR is a technique that provides high sensitivity for detection quantitative purposes.